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Exploring new functions of N2O-reducing bacteria by a novel culturing method and stable isotope probing: N2O mitigation and production of valuables

Research Project

Project/Area Number 20H04362
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 64020:Environmental load reduction and remediation-related
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Terada Akihiko  東京農工大学, 工学(系)研究科(研究院), 教授 (30434327)

Co-Investigator(Kenkyū-buntansha) 堀 知行  国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (20509533)
末永 俊和  広島大学, 先進理工系科学研究科(工), 助教 (80828377)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2022: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2021: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords亜酸化窒素 / 亜酸化窒素還元細菌 / 資源回収 / 微小呼吸活性 / メタゲノム解析 / 15Nトレーサー / 脱窒細菌 / メタン酸化 / N2O還元細菌 / 微小呼吸活性装置 / 集積培養 / 安定同位体 / 脱窒 / 安定同位体プローブ法 / 集積培養装置 / 有価物産生 / 有価物 / アップサイクリング / 有機炭素 / 動力学的解析 / 呼吸活性 / 物質産生
Outline of Research at the Start

本研究は、温室効果ガスである亜酸化窒素(N2O)の削減技術の確立に向け、N2Oを高効率に消費する細菌や、N2Oを消費しながら有価物を作ることが可能な細菌の獲得を目指す。高効率にN2Oを消費する細菌を集積するため、N2Oの精密な供給と制御が可能な装置の開発を行う。雑多な細菌群から有用種を集積する工程を通し、高効率にN2Oを消費できる細菌や、N2Oの消費と有価物の合成を達成する細菌の生き様に迫る。

Outline of Final Research Achievements

This study aimed at unraveling the physiological traits of efficient nitrous-oxide (N2O)-reducing bacteria and exploring the potential of bacteria able to synthesize value-added products by metagenomic analysis. Applying the 15N tracer approach and micro-respirometric assay allowed environmental conditions to fully harness clade II nosZ N2O-reducing bacteria for effective N2O mitigation. The bacteria showed high N2O affinity, whereas having a low availability of organic carbon species. Furthermore, microbial community and activity analyses of samples from an energy-saving nitrogen-removing reactor, a landfill treatment site, and rice paddies allowed the selection of efficient N2O-reducing bacteria potentially capable of synthesizing value-added production.

Academic Significance and Societal Importance of the Research Achievements

排水処理施設の窒素除去の過程で排出される強力な温室効果ガスである亜酸化窒素(N2O)は、より一層の排出削減が求められている。この課題に対する有力な手段として、N2OをN2に変換するN2O還元細菌の利用に期待が寄せられている。一方、この細菌の生理生態への理解は不十分である。さらに、近年の排水処理利施設は資源回収型への転換が求められている。本研究では、獲得したN2O還元細菌が優れたN2O消費活性を示す条件を明らかにした。さらに、有価物産生とN2O削減の双方を達成する微生物に迫る成果を得た。これらの成果は、排水処理施設からのN2O排出削減の達成や、有価物産生プロセスの開発に繋げられる可能性を有する。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (27 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (9 results) Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Int'l Joint Research] 韓国科学技術院(韓国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] ダルムシュタット工科大学(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] デンマーク工科大学(デンマーク)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] コロンビア大学(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 韓国科学技術院(韓国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 南京師範大学(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Technical University of Denmark(デンマーク)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Columbia University(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Technical University of Darmstadt(ドイツ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Exploring the functions of efficient canonical denitrifying bacteria as N2O sinks: Implications from 15N tracer and transcriptome analyses2022

    • Author(s)
      Kohei Oba, Toshikazu Suenaga, Megumi Kuroiwa, Shohei Riya, Akihiko Terada
    • Journal Title

      Environmental Science & Technology

      Volume: 56 Issue: 16 Pages: 11694-11706

    • DOI

      10.1021/acs.est.2c02119

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of nosZ-expressing microorganisms consuming trace N2O in microaerobic chemostat consortia dominated by an uncultured Burkholderiales2022

    • Author(s)
      Daehyun D. Kim, Heejoo Han, Taeho Yun, Min Joon Song, Akihiko Terada, Michele Laureni, Sukhwan Yoon
    • Journal Title

      The ISME Journal

      Volume: 16 Issue: 9 Pages: 2087-2098

    • DOI

      10.1038/s41396-022-01260-5

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficient oxygen supply and rapid biofilm formation by a new composite polystyrene elastomer membrane for use in a membrane-aerated biofilm reactor2022

    • Author(s)
      Kobayashi Masumi、Agari Ryosei、Kigo Yunje、Terada Akihiko
    • Journal Title

      Biochemical Engineering Journal

      Volume: 183 Pages: 108442-108442

    • DOI

      10.1016/j.bej.2022.108442

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organic carbon determines nitrous oxide consumption activity of clade I and II nosZ bacteria: Genomic and biokinetic insights2022

    • Author(s)
      Qi Chuang、Zhou Yiwen、Suenaga Toshikazu、Oba Kohei、Lu Jilai、Wang Guoxiang、Zhang Limin、Yoon Sukhwan、Terada Akihiko
    • Journal Title

      Water Research

      Volume: 209 Pages: 117910-117910

    • DOI

      10.1016/j.watres.2021.117910

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Nitrous oxide-sink capability of denitrifying bacteria impacted by nitrite and pH2022

    • Author(s)
      Zhou Yiwen、Zhao Shuting、Suenaga Toshikazu、Kuroiwa Megumi、Riya Shohei、Terada Akihiko
    • Journal Title

      Chemical Engineering Journal

      Volume: 428 Pages: 132402-132402

    • DOI

      10.1016/j.cej.2021.132402

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combination of 15N Tracer and Microbial Analyses Discloses N2O Sink Potential of the Anammox Community2021

    • Author(s)
      Suenaga Toshikazu、Ota Takumi、Oba Kohei、Usui Kentaro、Sako Toshiki、Hori Tomoyuki、Riya Shohei、Hosomi Masaaki、Chandran Kartik、Lackner Susanne、Smets Barth F.、Terada Akihiko
    • Journal Title

      Environmental Science & Technology

      Volume: 55 Issue: 13 Pages: 9231-9242

    • DOI

      10.1021/acs.est.1c00674

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Metagenomic Insights Into Functional and Taxonomic Compositions of an Activated Sludge Microbial Community Treating Leachate of a Completed Landfill: A Pathway-Based Analysis2021

    • Author(s)
      Yasuda Shohei、Suenaga Toshikazu、Orschler Laura、Agrawal Shelesh、Lackner Susanne、Terada Akihiko
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 948-948

    • DOI

      10.3389/fmicb.2021.640848

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Temperature and oxygen level determine N2O respiration activities of heterotrophic N2O‐reducing bacteria: Biokinetic study2020

    • Author(s)
      Zhou Yiwen, Suenaga Toshikazu, Qi Chuang, Riya Shohei, Hosomi Masaaki, Terada Akihiko
    • Journal Title

      Biotechnology and Bioengineering

      Volume: 118 Issue: 3 Pages: 1330-1341

    • DOI

      10.1002/bit.27654

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 新規ガス透過膜を用いた膜通気型バイオフィルムリアクターによる排水処理プロセスの開発2023

    • Author(s)
      三浦宏斗, 木子胤制, 寺田昭彦
    • Organizer
      第57回日本水環境学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 異なる脱窒酵素を有するアンモニア酸化細菌のN2O生成ポテンシャルの評価2023

    • Author(s)
      大石彬人, 武井大輝, 黒岩恵, 寺田昭彦
    • Organizer
      第57回日本水環境学会年会 2023年3月15日
    • Related Report
      2022 Annual Research Report
  • [Presentation] メタン酸化細菌による微生物タンパク質の産生:イネ根圏と土壌からの集積培養と性能評価2023

    • Author(s)
      徐 天祥, 安田昌平, 黒岩恵, 利谷 翔平, 大川泰一郎, 寺田昭彦
    • Organizer
      化学工学会第88年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Spatial distributions of transcriptionally active nitrous oxide-producing/reducing bacteria and microbial communities in a membrane-aerated biofilm reactor2022

    • Author(s)
      Maki Aizono, Kanako Mori, Kohei Oba, Megumi Kuroiwa, Shohei Riya, Tomoyuki Hori, Akihiko Terada
    • Organizer
      IWA BIOFILMS CONFERENCE 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hybrid Membrane-Aerated Biofilm Reactor (H-MABR) for Nitrogen Removal from a Mixture of Municipal and Agricultural Wastewater: Performances and Nitrifiers Communities2022

    • Author(s)
      Yunje Kigo, Tomoyuki Hori, Akihiko Terada
    • Organizer
      IWA BIOFILMS CONFERENCE 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ガス透過膜を用いた集積培養技術による高活性N2O 還元細菌群の集積化と活性評価2022

    • Author(s)
      大場康平,黒岩恵,利谷翔平,寺田昭彦
    • Organizer
      第56回日本水環境学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] メタン酸化細菌 Methylomicrobium によるメタンからのエクトイン産生ポテンシャルの評価2022

    • Author(s)
      小笠原 瑠里,安田 昌平,黒岩 恵,利谷 翔平,寺田 昭彦
    • Organizer
      化学工学会第87年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Growth optimization and biokinetic characterization of a novel bioplastic-producing, methane-oxidizing bacterium, Methylosinus sp. C492022

    • Author(s)
      Magomnang Jameil Jr. Decatoria, Yasuda Shohei, Kuroiwa Megumi, Riya Shohei, Terada Akihiko
    • Organizer
      化学工学会第87年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 15N トレーサー法を用いた脱窒細菌のN2O 生成・消費ポテンシャルの定量化とメカニズム解明2021

    • Author(s)
      大場康平,末永俊和,黒岩恵,寺田昭彦
    • Organizer
      第24回日本水環境学会シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Membrane-aerated biofilm as a way to mitigate N2O emissions during nitrogen removal2020

    • Author(s)
      Akihiko Terada
    • Organizer
      2020 Biofilms Virtual Conference
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2020-04-28   Modified: 2024-01-30  

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