• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a drug assessment method based on the function of human cerebral organoids

Research Project

Project/Area Number 20H04507
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 90110:Biomedical engineering-related
Research InstitutionTohoku Institute of Technology

Principal Investigator

Suzuki Ikuro  東北工業大学, 大学院工学研究科, 教授 (90516311)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2021: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2020: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywordsヒト脳オルガノイド / MEA / 低周波成分解析 / 神経伝達物質 / 同時計測 / 細胞外電位計測 / 電気化学計測 / 創薬 / 脳オルガノイド / ヒトiPS細胞 / 微小電極アレイ / 電気活動 / カーボンナノチューブ / オシレーション / 薬効評価
Outline of Research at the Start

ヒト大脳皮質の3次元構造を一部再現できる「脳オルガノイド」を用いて、神経疾患患者の大脳皮質で見られるオシレーション強度の違いを指標とした薬効評価法、ヒト脳モデルのLTP試験法、および細胞外電位と神経伝達物質放出を同時検出可能な評価系の構築を目指す。

Outline of Final Research Achievements

In microelectrode array (MEA) measurements of human brain organoids, we showed that electrical activity characteristics and compound responses are more pronounced in the low-frequency component below 500 Hz, rather than in the conventional spike component. Frequency characteristics reflecting in vivo and contraindicated drug responses were also detected in diseased brain organoids. Furthermore, the development of carbon nanotube MEAs and an MEA system capable of simultaneous electrochemical measurements enabled real-time detection of glutamate release and extracellular potentials.

Academic Significance and Societal Importance of the Research Achievements

動物実験代替とヒトへの外挿性を有するヒト脳オルガノイドの機能評価法を開発し、疾患脳オルガノイドの電気活動特性および禁忌薬の応答を見出した成果は、今後の創薬開発に大きく貢献する成果である。神経伝達物質の放出と細胞外電位の同時計測法は、神経応答のメカニズムにアプローチできる新規計測法であり、学術的意義は高く、医薬品をはじめとする化合物評価において実用化が期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (38 results)

All 2023 2022 2021 2020

All Journal Article (14 results) (of which Peer Reviewed: 14 results,  Open Access: 10 results) Presentation (21 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results) Book (3 results)

  • [Journal Article] Large-Area Field Potential Imaging Having Single Neuron Resolution Using 236 880 Electrodes CMOS-MEA Technology2023

    • Author(s)
      1.I. Suzuki*, N. Matsuda, X. Han, S. Noji, M. Shibata, N. Nagafuku, Y. Ishibashi
    • Journal Title

      Advanced Science

      Volume: なし Issue: 20 Pages: 1-23

    • DOI

      10.1002/advs.202207732

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Assessment of astrocytic electrophysiological responses to seizurogenic compounds using planner microelectrode array2023

    • Author(s)
      T. Kuroda, N. Matsuda, Y. Ishibashi, I Suzuki*
    • Journal Title

      Frontiers in Neuroscience

      Volume: 16 Pages: 1-17

    • DOI

      10.3389/fnins.2022.1050150

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ヒトiPS神経の電気活動に基づいた化合物の毒性及び作用機序予測2022

    • Author(s)
      鈴木郁郎
    • Journal Title

      谷本学校 毒性質問箱

      Volume: 24 Pages: 20-31

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A functional neuron maturation device provides convenient application on microelectrode array for neural network measurement2022

    • Author(s)
      3.X Han, N Matsuda, Y Ishibashi, A Odawara; S Takahashi; N Tooi, K Kinoshita, I Suzuki*
    • Journal Title

      Biomaterials Research

      Volume: 26(84) Issue: 1 Pages: 1-17

    • DOI

      10.1186/s40824-022-00324-z

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An <i>in vitro</i> microfluidic culture device for peripheral neurotoxicity prediction at low concentrations based on deep learning2022

    • Author(s)
      X Han, N Matsuda, K Matsuda, M Yamanaka, I Suzuki*
    • Journal Title

      Fundamental Toxicological Sciences

      Volume: 9 Issue: 7 Pages: 203-209

    • DOI

      10.2131/fts.9.203

    • ISSN
      2189-115X
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Responses to antibiotics in human iPSC-derived neurons based on the clinical antibiotic-associated encephalopathy classification2022

    • Author(s)
      Y. Ishibashi, S. Kimura, I Suzuki*
    • Journal Title

      The Journal of Toxicological Sciences

      Volume: 47 Issue: 10 Pages: 429-437

    • DOI

      10.2131/jts.47.429

    • ISSN
      0388-1350, 1880-3989
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In Vitro Pain Assay Using Human iPSC-Derived Sensory Neurons and Microelectrode Array.2022

    • Author(s)
      6.A Odawara, M Shibata, Y Ishibashi, N Nagafukum N matsuda, I Suzuki*
    • Journal Title

      Toxicological Sciences

      Volume: 188(1) Issue: 1 Pages: 131-141

    • DOI

      10.1093/toxsci/kfac045

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Raster plots machine learning to predict the seizure liability of drugs and to identify drugs2022

    • Author(s)
      N Matsuda, A Odawara, Y Ishibashi, K Kinoshita, A Okamura, T Shirakawa, I Suzuki
    • Journal Title

      Sci Rep

      Volume: 12(1) Issue: 1 Pages: 2281-2281

    • DOI

      10.1038/s41598-022-05697-8

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Electrophysiological responses to seizurogenic compounds dependent on E/I balance in human iPSC-derived cortical neuronal networks2022

    • Author(s)
      R Yokoi, T Kuroda, N Matsuda, A Odawara, I Suzuki
    • Journal Title

      Journal of Pharmacological Sciences

      Volume: 148(2) Issue: 2 Pages: 267-278

    • DOI

      10.1016/j.jphs.2021.12.006

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of signal components < 500 Hz in brain organoids coupled to microelectrode arrays: a reliable test- bed for preclinical seizure liability assessment of drugs and screening of antiepileptic drugs2021

    • Author(s)
      Yokoi R, Shibata M, Odawara A, Ishibashi Y, Nagafuku N, Matsuda N, Suzuki I
    • Journal Title

      Biochem and Biophys Res.

      Volume: 28 Pages: 101148-101148

    • DOI

      10.1016/j.bbrep.2021.101148

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Histograms of Frequency-Intensity Distribution Deep Learning to Predict the Seizure Liability of Drugs in Electroencephalography.2021

    • Author(s)
      N. Matsuda, Kenichi Kinoshita, Ai Okamura, Takafumi Shirakawa, and I Suzuki
    • Journal Title

      Toxicological Sciences

      Volume: 182(2) Issue: 2 Pages: 229-242

    • DOI

      10.1093/toxsci/kfab061

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Principal Component Analysis to Distinguish Seizure Liability of Drugs in Human iPS Cell-Derived Neurons2021

    • Author(s)
      Y Ishibashi, A Odawara, K Kinoshita, A Okamura, T Shirakawa, I Suzuki
    • Journal Title

      Toxicological Sciences

      Volume: 184(2) Issue: 2 Pages: 265-275

    • DOI

      10.1093/toxsci/kfab116

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Can We Panelize Seizure?2021

    • Author(s)
      1.R Roberts, S Authier,D Mellon, M Morton, I Suzuki, RB Tjalkens, JP Valentin, JB Pierson
    • Journal Title

      Toxicological Sciences

      Volume: 179 Issue: 1 Pages: 3-13

    • DOI

      10.1093/toxsci/kfaa167

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Versatile live-cell activity analysis platform for characterization of neuronal dynamics at single-cell and network level2020

    • Author(s)
      Yuan X, Schroter M, Obien MEJ, Fiscella M1, Gong W, Kikuchi T, Odawara A, Noji S, Suzuki I, Takahashi J, Hierlemann A, Frey U
    • Journal Title

      Nature communication

      Volume: 11 Issue: 1 Pages: 1-14

    • DOI

      10.1038/s41467-020-18620-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] in vitro神経活動に基づいた医薬品評価2022

    • Author(s)
      鈴木郁郎
    • Organizer
      シンポジウム「 次世代ICTと未来医療を支える 神経科学・神経工学・ 脳型コンピューティング 」第83回応用物理学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] ヒトiPS神経のMEA計測による化合物の毒性リスク予測2022

    • Author(s)
      鈴木郁郎
    • Organizer
      情報計算化学生物学会(CBI学会) 2022年大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] in vitro 神経活動に基づいた化学物質の神経毒性評価2022

    • Author(s)
      鈴木郁郎
    • Organizer
      シンポジウム「発達神経毒性の現状と今後の課題」 第35回日本動物実験代替法学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Ca-transientsを神経活動指標とした化合物の毒性リスク予測2022

    • Author(s)
      鈴木郁郎
    • Organizer
      スクリーニング研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Development of the Prediction Method of Peripheral Neuropathy Based on Firing Pattern of Each Single Neuron2022

    • Author(s)
      Xiaobo Han, Naoki Matsuda, Mikako Shibata, Ikuro Suzuki
    • Organizer
      Safety Pharmacology Society 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Toxicity risk assessment method for compounds using human iPS cell-derived neurons2022

    • Author(s)
      Yuto Ishibashi, Nami Nagafuku, Ikuro Suzuki
    • Organizer
      Neuroscience2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a microfluidic culture device for in vitro neural toxicity assessment with AI image analysis2022

    • Author(s)
      Xiaobo Han, Naoki Matsuda, Makoto Yamanaka, Ikuro Suzuki
    • Organizer
      Neuroscience 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] MEAを用いた化合物の神経毒性評価法の開発2022

    • Author(s)
      鈴木郁郎
    • Organizer
      電気化学会第89回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] MEAを使用した脳オルガノイド計測における低周波解析の有効性2022

    • Author(s)
      永福菜美, 横井れみ, 柴田実可子, 石橋勇人, 松田直毅, 鈴木郁郎
    • Organizer
      第13回日本日本安全性薬理研究会学術年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Evaluation of drug toxicity and efficacy based on human iPSC-derived neuronal activities2021

    • Author(s)
      鈴木郁郎
    • Organizer
      第62回 日本神経学会学術大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] In vitro, ex vivo, in vivoにおける神経活動を指標とした薬剤性痙攣リスク評価2021

    • Author(s)
      鈴木郁郎
    • Organizer
      48回日本毒性学会学術年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヒトiPS細胞由来ニューロンおよび脳オルガノイドを用いた医薬品の毒性評価2021

    • Author(s)
      鈴木郁郎
    • Organizer
      48回日本毒性学会学術年会 ランチョンセミナー
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヒトiPS細胞由来神経細胞の電気活動に基づいた毒性および薬効評価2021

    • Author(s)
      鈴木郁郎
    • Organizer
      48回日本毒性学会学術年会 ランチョンセミナー
    • Related Report
      2021 Annual Research Report
  • [Presentation] iPSニューロンの機能を指標とした中枢および末梢神経毒性評価2021

    • Author(s)
      鈴木郁郎
    • Organizer
      安全性評価研究会 第29回 夏の教育フォーラム
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヒト iPS 神経の電気活動に基づいた医薬品候補化合物の評価2021

    • Author(s)
      鈴木郁郎
    • Organizer
      令和3年度AMED 再生・細胞医療・遺伝子治療研究開発交流会
    • Related Report
      2021 Annual Research Report
  • [Presentation] MEA measurement of human cerebral organoid2021

    • Author(s)
      Mikako Shibata, Remi Yokoi, Aoi Odawara, Yuto Ishibashi, Nami Nagafuku, Naoki Matsuda, Ikuro Suzuki
    • Organizer
      2021 Safety Pharmacology Society
    • Related Report
      2021 Annual Research Report
  • [Presentation] 脳オルガノイドのMEA計測2021

    • Author(s)
      横井 れみ, 石橋 勇人, 松田 直毅, 永福 菜美, 鈴木 郁郎
    • Organizer
      第94回日本薬理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 神経機能を指標としたインビトロ毒性評価試験とAI2020

    • Author(s)
      鈴木郁郎
    • Organizer
      第47回日本毒性学会学術年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] in vitroヒト神経機能を指標とした創薬利活用2020

    • Author(s)
      鈴木郁郎
    • Organizer
      第58回日本人工臓器学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒト大脳皮質オルガノイドにおける痙攣陽性化合物に対する応答2020

    • Author(s)
      小田原 あおい, 松田 直毅, 石橋 勇人, 横井 れみ, 鈴木 郁郎
    • Organizer
      第47回日本毒性学会学術年
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒト脳オルガノイドのMEA計測による痙攣陽性化合物の応答評価2020

    • Author(s)
      横井れみ,石橋勇人,松田直毅,小田原あおい,鈴木郁郎
    • Organizer
      第27回HAB研究機構学術年会
    • Related Report
      2020 Annual Research Report
  • [Book] ペランパネルによるてんかん治療のストラテジー:第2版 Part 1.分子病態から探るてんかん原性メカニズム “脳オルガノイドを用いたてんかん原性研究”2022

    • Author(s)
      鈴木郁郎
    • Total Pages
      175
    • Publisher
      先端医学社
    • ISBN
      9784865505580
    • Related Report
      2022 Annual Research Report
  • [Book] ヒトiPS神経の機能を指標とした医薬品の神経毒性予測および薬効評価への取り組み2020

    • Author(s)
      鈴木郁郎
    • Total Pages
      6
    • Publisher
      日本薬理学会誌
    • Related Report
      2020 Annual Research Report
  • [Book] ヒトiPS細胞由来神経細胞を用いた痙攣評価系の検討2020

    • Author(s)
      白川誉史, 鈴木郁郎
    • Total Pages
      6
    • Publisher
      日本薬理学会誌
    • Related Report
      2020 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi