| Project/Area Number |
20J10766
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 58020:Hygiene and public health-related: including laboratory approach
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| Research Institution | Hokkaido University |
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Principal Investigator |
KETEMA RAHEL MESFIN 北海道大学, 保健科学院, 特別研究員(DC2)
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| Project Period (FY) |
2020-04-24 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2020: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Biomonitoring / Phthalate esters / School children / Allergies / Oxidative stress markers / phthalates exposure / children / exposure trend / Lifestyle behaviors / biomonitoring / regulation |
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| Outline of Research at the Start |
First part of this research outline is to explore level of phthalates exposure trend between 2012-2017 in 7 years old children from Hokkaido. The second part we will conduct a case-control epidemiological study to investigate phthalate exposure association with oxidative stress and asthma disease.
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| Outline of Annual Research Achievements |
Urine was collected from 7-year-old children participating in the Hokkaido birth cohort, and 10 metabolites of 4 phthalate compounds were measured by UPLC-MS/MS. Levels of 3 oxidative stress markers (8-OHdG, HEL, HNE)) were measured by ELISA kits. ISAAC questionnaire completed by parents of the child used to define wheeze, rhino conjunctivitis, and eczema.
All metabolites were detected in > 96% of samples. Highest detection was in MECPP, MnBP, and MEHHP with median 37.4 ng/mL, 36.8 ng/mL, and 25.8 ng/mL, respectively. No exposure change was observed in these phthalate metabolites from 2012 to 2017. The Japan phthalate ester regulation revised in 2010 in toys and food containers related to children could not alter the phthalates exposure change. Individual phthalate metabolites, MECPP (OR = 1.41) and cx-MINP (OR = 1.40) increased the risk of wheeze. In a mixed exposure analysis, the Weighted Quantile Sum index showed significant association (OR = 1.46) with wheeze and (OR = 1.40) eczema. The Bayesian Kernel Machine Regression model, DINP metabolites showed the highest group posterior inclusion probability (PIP). Among DINP metabolites, MINP in wheeze, cx-MINP in rhino-conjunctivitis and OH-MINP in eczema showed the highest conditional PIPs. The overall metabolites mixture effect was associated with eczema. On the contrary, no mediation of oxidative stress was observed in the association between phthalates and symptoms, which may be due to low oxidative stress. Participants in this study were only 7 years old, demonstrating the importance of continuing the study in the future.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(7 results)
