Development of novel transfection system using DNA gel
| Project/Area Number |
20J11059
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 62010:Life, health and medical informatics-related
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MASUKAWA Marcos 東京工業大学, 情報理工学院, 特別研究員(DC2)
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| Project Period (FY) |
2020-04-24 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2020: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | DNA origami / colloidosome / self-assembly / aqueous two-phase system / aqueous-aqueous emulsion / DNA hydrogel / purification / monodisperse / microfluidic |
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| Outline of Research at the Start |
DNA and proteins can be inserted into the cells to modify their metabolism and genetic material. This process is called transfection and it is a key technique needed in gene editing, a prospective cure for degenerative diseases. To increase the functionality of transfected structures, we are developing a DNA hydrogel whose biological function can be activated by a chemical signal. Inside the cells, these structures can perform as molecular computers, processing signals and producing outputs within the cell. These cell hybrids will lead to novel diagnostics and treatments.
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| Outline of Annual Research Achievements |
In recent research, the interaction between self-assembled DNA structures and liquid-liquid phase-separated systems composed of polymers was investigated. It was observed that DNA structures can be selectively taken up by these compartments, leading to self-assembly that mimics artificial cells and the interaction of nucleic acids and organelles. Based on these findings, I developed a method for purifying self-assembled DNA structures and created a new category of colloidossomes based on the accumulation of DNA origami at the interface of droplets. This enabled the creation of colloidosomes that can self-organise into cellular like structures. In the future, I will explore the possibility of translocating nucleic through membranes of DNA coloidosomes, which might find application in DNA sequencing technology and on synthetic cell models for the transport of nucleic acids thourgh membranes.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(10 results)
