Development of Novel mRNA Delivery Systems based on Flexible Biodegradable Block Copolymers
| Project/Area Number |
20J13458
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 90120:Biomaterials-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
楊 文セイ 東京大学, 工学系研究科, 特別研究員(DC2)
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| Project Period (FY) |
2020-04-24 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2021: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Nanomedicine / Polymeric micelles / messenger RNA |
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| Outline of Research at the Start |
本研究ではmRNAの安定化とポリマーの生分解性に焦点を当てた検討から着手し、機能性アミノ酸を導入することにより、mRNAに安定効果もあり、かつ標的細胞、部位、至るところの毒性を減低する。本研究は、ポリマー生分解性とmRNA 安定化効果の両方を兼ね備えたシステムを構築する際の優れた設計指針となり、この技術により多種ヌクレオチト薬剤を全身へ送達することが可能となり、がんを含めて様々難治療疾患の治療や診断への展開が期待できる。
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| Outline of Annual Research Achievements |
Here, we focused on the development of nanocarriers for safe and stable systemic delivery of mRNA. Thus, we have developed a library of polymers, and chemically modified with amino acids which could stable mRNA by hydrophobic interaction and π-π interaction. We confirmed that these polymers were biodegradable in physiological conditions. Polymeric micelles prepared by polymers with π-π interaction were found to be safe in vitro after incubation with 50% Fetal Bovine Serum, could achieve a highest mRNA expression in vitro. We found that micelles with π-π interaction with mRNA showed higher protein expression in the lung of mouse after 24 h intratracheal administration. Moreover, these micelles maintained over 10% of loaded mRNA in mice blood after 10 mins of intravenous administration.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(7 results)
