分子認識PEGゲルキャピラリー電気泳動による生体分子の構造・サイズ選択的分離
| Project/Area Number |
20J15151
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 34020:Analytical chemistry-related
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| Research Institution | Kyoto University |
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Principal Investigator |
劉 晨晨 京都大学, 工学研究科, 特別研究員(PD)
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| Project Period (FY) |
2020-04-24 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2021: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2020: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | poly(ethylene glycol) / protein analysis / affinity electrophoresis / electrophoresis / DNA analysis |
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| Outline of Research at the Start |
A poly(ethylene glycol) (PEG) gel-based molecularly imprinted polymer (MIP) is studied aiming at enhanced separation performance for proteins. The gel-based MIP is promising in separation science but suffers from low reproducibility. For solving this issue, a novel PEG gel with enhanced mechanical property will be investigated.
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| Outline of Annual Research Achievements |
The poly(ethylene glycol) (PEG) based molecularly imprinted hydrogel (MIH) was fabricated for the molecular recognition of the target protein. Copoly(PEG acrylate/PEG diacrylate) (copoly(PEGA/PEGDA)) hydrogel was employed in the preparation of MIH for cytochrome c recognition. A highly specific molecular recognition was realized when using the MIH as a sorbent, evidenced by (1) the MIH showed adsorption to the cytochrome c target while the non-imprinted hydrogel showed no adsorption to the target, and (2) the MIH hydrogel showed higher adsorption to the cytochrome c target than to the other unspecific proteins. When tested as the affinity substance in electrophoresis, the MIH retarded the migration of cytochrome c to a higher extent compared with the other unspecific proteins.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(8 results)
