マイクロヒーター・ナノワイヤを用いた分子交雑に基づくDNA配列解析法の創出
| Project/Area Number |
20J15564
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 28040:Nanobioscience-related
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| Research Institution | Nagoya University |
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Principal Investigator |
高橋 博美 名古屋大学, 工学研究科, 特別研究員(DC2)
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| Project Period (FY) |
2020-04-24 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2020: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | nanowires / microfluidic / nucleic acid / ZnO nanowires / Microfluidic |
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| Outline of Research at the Start |
With circulating free DNA (cfDNA) hybridization-based analysis, most genetic diseases can be identified. However, challenging factors are: (1) the low concentration and small size of cfDNA, and (2) the reannealing between a complementary single-stranded DNA (ssDNA) resulting in lower efficiency analysis. Thus, a device that can retain ssDNA in room temperature will enhance the sensitivity. Here we purpose microheater nanowire microfluidic devices for analyzing methylated cfDNA via the combination of capturing ssDNA on nanowire surfaces, followed-by sequence-specific detection of target DNA.
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| Outline of Annual Research Achievements |
The development of nanowire devices integrated with a microheater (Microheater - ZnO nanowire device) as nucleic acid detection platform using in situ annealed Serinol Nucleic Acid (SNA) probes was demonstrated. First, the microheater could constantly generate suitable heat was confirmed. Second, the use of SNA-MBs to detect target ssDNAs specifically was confirmed. By combining the unique properties of ZnO-NWs and the microheater, the integrated device could detect target ssDNAs using in-situ annealed probes was confirmed. This confirms that this microheater-ZnO-NW device will be applied to fundamental studies related to nanobiodevice-based DNA detection.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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Report
(2 results)
Research Products
(4 results)
