| Project/Area Number |
20K05321
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 29020:Thin film/surface and interfacial physical properties-related
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
YURTSEVER AYHAN 金沢大学, ナノ生命科学研究所, 特任助教 (00761529)
|
|---|
| Project Period (FY) |
2020-04-01 – 2024-03-31
|
| Project Status |
Completed (Fiscal Year 2023)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | Exosomes / 3D-AFM / Nanomechanics / exosomes / Atomic force microscopy / Cells / Atomic force Microscopy / Cell / AFM |
|---|
| Outline of Research at the Start |
The aim of this research proposal is to reveal the detailed structure and mechanics of exosomes derived from different cell types using 3D-atomic force microcopy (3D-AFM) operating in a liquid environment. We intend to determine the possible differences between normal and cancer-derived exosomes in terms of their structural, molecular, and biomechanical characteristics.
|
| Outline of Final Research Achievements |
We employed 3D-AFM to reveal the structural and associated-nanomechanical properties of exosomes. 3D-AFM showed the presence of distinct nanodomains bulging out from the membrane surface, which can be attributed to the heterogeneous presence of membrane-associated proteins exposed on the vesicles’ outer surfaces. Our results further showed differences between the nanomechanical properties of metastatic and nonmetastatic cancer cell-derived exosomes.
|
| Academic Significance and Societal Importance of the Research Achievements |
Cancer is a leading cause of death due to late diagnosis. Distinguishing between metastatic and non-metastatic malignant cell-derived exosomes is crucial. This differentiation aids in understanding exosome functions and enhances their use as biomarkers, improving early cancer detection and outcomes.
|
