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Cultivation and molecular biological analysis of copper reducing bacteria as for a copper recycling from low-grade metal ore

Research Project

Project/Area Number 20K05407
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 31020:Earth resource engineering, Energy sciences-related
Research InstitutionMeijo University

Principal Investigator

Hosoda Akifumi  名城大学, 農学部, 准教授 (50434618)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2022: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords銅イオン還元 / 嫌気性細菌 / Alteromonas科 / Pseudomonas科 / 硫化銅 / 酸化銅 / 次世代シークエンス / Alteromonas / Shewanella科 / 銅還元微生物 / バイオリーチング / 嫌気培養 / 電子メディエーター / 鉱物廃材
Outline of Research at the Start

これまでに申請者は,硫化銅(Ⅰ)を含む土壌から酢酸を利用し嫌気的に銅を還元する嫌気性銅還元細菌群集(以下,マイクロコズム)を構築してきた.本研究では,このマイクロコズムに生息する銅還元に関与する微生物を次世代シークエンス等の分子生物学的手法で同定し,その情報と還元能力を補助する物質(電子メディエーター等)の添加に基づいた培養により新たな銅還元微生物として獲得する.また,得られた微生物の分子生物学・生化学的解析より銅還元に関わる遺伝子の特定と銅還元メカニズムを明らかにする.さらに,鉱物廃材(あるいは廃電子基板)と得られた微生物による培養条件の検討を行い,実用的な銅リサイクルの新技術開発を目指す.

Outline of Final Research Achievements

Anaerobic copper-ion reducing microcosms were constructed from river sediment or marine sludge. Next generation sequencing analyses revealed that dominant microbes were Pseudomonadaceae, Alteromonadaceae, Rhodocylaseae and Shewanellaceae. Considering that some bacterial strains which could reduce metals were affiliated with these bacterial families, it was revealed that these bacteria had resistance to copper and reduction of copper-ion under anaerobic condition in the microcosms. However, this research project still has some problems in the difficulty of stable subculturing and constructing of microcosms that could reduce copper-ion up to a practical copper recycling concentration.

Academic Significance and Societal Importance of the Research Achievements

本研究で構築したマイクロコズムにおける銅還元能は1.5 mM程度でAlteromonas科やPseudomonas科が優占化したことが分かった.これらの結果は,現在行われている銅リサイクルに適用可能な濃度(5000 ppm)ではないが,本研究で構築した銅還元-嫌気性マイクロコズム中の細菌を環境から分離後,さらに銅還元遺伝子などを付与するといった遺伝子改変・育種によってエネルギー消費が少ない条件での生物的銅リサイクルについての可能性を有していると考えられる.

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (6 results)

All 2022 2021

All Journal Article (2 results) (of which Open Access: 1 results,  Peer Reviewed: 1 results) Presentation (4 results)

  • [Journal Article] Bacteria improves copper recycling processes: Recycling electronic waste in efficient and novel ways2022

    • Author(s)
      Akifumi Hosoda
    • Journal Title

      Impact

      Volume: 1 Issue: 1 Pages: 48-50

    • DOI

      10.21820/23987073.2022.1.48

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Open Access
  • [Journal Article] Correlation between molecular microbial community and nitrogen cycling on ornithogenic soil affected by tsunami in Japan2022

    • Author(s)
      Hosoda Akifumi、Kurosaki Mao、Kazama Kentaro、Murano Hirotatsu、Mizota Chitoshi、Niizuma Yasuaki
    • Journal Title

      Ecological Genetics and Genomics

      Volume: 23 Pages: 100114-100114

    • DOI

      10.1016/j.egg.2022.100114

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed
  • [Presentation] 海洋底泥中の銅還元嫌気性細菌の生態解析2022

    • Author(s)
      林 奏希,松尾 健太郎,細田 晃文
    • Organizer
      日本微生物生態学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 鉄還元能を有する糸状菌の生理的特性2022

    • Author(s)
      小島 史大, 杉本 陸飛,横井 裕伎,松岡 七海,細田 晃文
    • Organizer
      日本微生物生態学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 河川底泥からの嫌気性‐銅酸化/還元微生物の集積培養と分子生態解析2021

    • Author(s)
      榎本圭佑,細田晃文
    • Organizer
      日本微生物生態学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 汽水域底泥から集積した硝酸還元性鉄酸化細菌の分子生態解析2021

    • Author(s)
      堀内穂孝,林田真貴子,細田晃文
    • Organizer
      日本微生物生態学会
    • Related Report
      2021 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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