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Elucidation of long-distance signals that transmit nitrogen nutritional status from shoot to root

Research Project

Project/Area Number 20K05771
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionShimane University

Principal Investigator

Hachiya Takushi  島根大学, 学術研究院農生命科学系, 助教 (80709311)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords長距離シグナル / 全身的応答 / 窒素充足 / 硝酸シグナル / サイトカイニン / 転写因子 / 窒素充足応答
Outline of Research at the Start

我々は、植物が地上部の硝酸イオン濃度によって自身の窒素栄養状態(腹の減り具合)を感知し、根の成長・高親和性硝酸イオン輸送体の発現(根の窒素吸収能力)を制御することを見出している。地上部の硝酸イオン濃度の情報を根に伝達するためには、長距離移動型の情報分子(長距離シグナル)が必要である。本研究では、この長距離シグナルを同定することによ
り、植物体の全身的な窒素シグナル伝達経路の解明を目指す。

Outline of Final Research Achievements

In this study, we aimed to elucidate the signals that convey the nitrogen nutritional status from shoot to root in plants. Based on preliminary data, we focused on repressor X mRNA and isopentenyl cytokinin (iP-CK) as candidate signals. For this study, we constructed a simple and nondestructive nutritional manipulation transplantation system and proceeded with the analysis. As a result, no clear evidence was obtained that repressor X mRNA and iP-CK functioned as a signal. On the other hand, we found that the accumulation of nitrate ions in the shoot promoted shoot growth and defense responses mediated by iP-CK signaling, and that root-specific reduction of CK signaling promoted shoot growth.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、地上部の硝酸イオンレベルの上昇にともなってイソペンテニル型サイトカイニンが合成されることがわかった。さらにイソペンテニル型サイトカイニンのシグナルを介して成長や防御応答が促進されることもわかった。これにより、植物が自身の窒素栄養状態に応じて成長や防御応答を制御する仕組みの一端がわかった。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (24 results)

All 2023 2022 2021 Other

All Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 10 results,  Open Access: 7 results) Presentation (11 results) (of which Invited: 3 results) Remarks (3 results)

  • [Journal Article] Targeted expression of bgl23-D, a dominant-negative allele of ATCSLD5, affects cytokinesis of guard mother cells and exine formation of pollen in Arabidopsis thaliana2023

    • Author(s)
      Hossain Md. Firose、Dutta Amit Kumar、Suzuki Takamasa、Higashiyama Tetsuya、Miyamoto Chiharu、Ishiguro Sumie、Maruta Takanori、Muto Yuki、Nishimura Kohji、Ishida Hideki、Aboulela Mostafa、Hachiya Takushi、Nakagawa Tsuyoshi
    • Journal Title

      Planta

      Volume: 257 Issue: 4 Pages: 64-82

    • DOI

      10.1007/s00425-023-04097-0

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Root-specific activation of plasma membrane H+-ATPase 1 enhances plant growth and shoot accumulation of nutrient elements under nutrient-poor conditions in Arabidopsis thaliana2022

    • Author(s)
      Monden Kota、Kamiya Takehiro、Sugiura Daisuke、Suzuki Takamasa、Nakagawa Tsuyoshi、Hachiya Takushi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 621 Pages: 39-45

    • DOI

      10.1016/j.bbrc.2022.06.097

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Systemic Regulation of Iron Acquisition by Arabidopsis in Environments with Heterogeneous Iron Distributions2022

    • Author(s)
      Ryo Tabata, Takehiro Kamiya, Shunpei Imoto, Hana Tamura, Michika Tabata, Tasuku Hirayama, Hironaka Tsukagoshi, Keitaro Tanoi, Takamasa Suzuki, Takushi Hachiya, Hitoshi Sakakibara
    • Journal Title

      Plant Cell Physiology

      Volume: - Issue: 6 Pages: 842-854

    • DOI

      10.1093/pcp/pcac049

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation of ammonium acquisition and use in Oryza longistaminata ramets under nitrogen source heterogeneity2022

    • Author(s)
      Misato Kawai, Ryo Tabata, Miwa Ohashi, Haruno Honda, Takehiro Kamiya, Mikiko Kojima, Yumiko Takebayashi, Shunsuke Oishi, Satoru Okamoto, Takushi Hachiya, Hitoshi Sakakibara
    • Journal Title

      Plant Physiology

      Volume: 188 Issue: 4 Pages: 2364-2376

    • DOI

      10.1093/plphys/kiac025

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana2022

    • Author(s)
      Hossain Md. Firose、Sultana Mst Momtaz、Tanaka Ai、Dutta Amit Kumar、Hachiya Takushi、Nakagawa Tsuyoshi
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 30 Pages: 101241-101241

    • DOI

      10.1016/j.bbrep.2022.101241

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Root-specific Reduction of Cytokinin Perception Enhances Shoot Growth in <i>Arabidopsis thaliana</i>2022

    • Author(s)
      Monden Kota、Kojima Mikiko、Takebayashi Yumiko、Suzuki Takamasa、Nakagawa Tsuyoshi、Sakakibara Hitoshi、Hachiya Takushi
    • Journal Title

      Plant and Cell Physiology

      Volume: 63 Issue: 4 Pages: 484-493

    • DOI

      10.1093/pcp/pcac013

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana2021

    • Author(s)
      Hachiya Takushi、Inaba Jun、Wakazaki Mayumi、Sato Mayuko、Toyooka Kiminori、Miyagi Atsuko、Kawai-Yamada Maki、Sugiura Daisuke、Nakagawa Tsuyoshi、Kiba Takatoshi、Gojon Alain、Sakakibara Hitoshi
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 4944-4944

    • DOI

      10.1038/s41467-021-25238-7

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nitrogen Nutrition Promotes Rhizome Bud Outgrowth via Regulation of Cytokinin Biosynthesis Genes and an Oryza longistaminata Ortholog of FINE CULM 12021

    • Author(s)
      Shibasaki Kyohei、Takebayashi Arika、Makita Nobue、Kojima Mikiko、Takebayashi Yumiko、Kawai Misato、Hachiya Takushi、Sakakibara Hitoshi
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 670101-670101

    • DOI

      10.3389/fpls.2021.670101

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A cellophane-supported Arabidopsis culture for seamless transfer between different media is useful for studying various nitrogen responses2021

    • Author(s)
      Hachiya Takushi、Oya Takahiro、Monden Kouta、Nagae Ami、Nakagawa Tsuyoshi
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 未定 Issue: 3 Pages: 1-6

    • DOI

      10.1080/00380768.2021.1908094

    • NAID

      210000158977

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Temperature-dependent fasciation mutants provide a link between mitochondrial RNA processing and lateral root morphogenesis2021

    • Author(s)
      Otsuka Kurataka、Mamiya Akihito、Konishi Mineko、Nozaki Mamoru、Kinoshita Atsuko、Tamaki Hiroaki、Arita Masaki、Saito Masato、Yamamoto Kayoko、Hachiya Takushi、Noguchi Ko、Ueda Takashi、Yagi Yusuke、Kobayashi Takehito、Nakamura Takahiro、Sato Yasushi、Hirayama Takashi、Sugiyama Munetaka
    • Journal Title

      eLife

      Volume: 10

    • DOI

      10.7554/elife.61611

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 地上部で合成されるイソペンテニル アデニン型サイトカイニンの新たな 生理機能の解明2023

    • Author(s)
      門田宏太, 小嶋美紀子, 竹林裕美子, 鈴木孝征, 杉浦大輔, 中川強, 榊原 均, 蜂谷卓士
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] シロイヌナズナ硝酸トランスセプター NRT1.1/NPF6.3 の硝酸非依存的機能に関する包括的解析2023

    • Author(s)
      蜂谷卓士, 中川強, 榊原均
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 地上部の硝酸イオン充足により合成されるイソペンテニルアデニン型サイトカイニンの新たな生理機能の解明2022

    • Author(s)
      門田宏太, 蜂谷卓士
    • Organizer
      植物の栄養研究会 第7回研究交流会
    • Related Report
      2022 Annual Research Report
  • [Presentation] シロイヌナズナにおける地上部で合成されるイソペンテニルアデニン型サイトカイニンの新たな生理機能の解明2022

    • Author(s)
      門田 宏太, 小嶋 美紀子, 竹林 裕美子, 鈴木 孝征, 中川 強, 榊原 均, 蜂谷 卓士
    • Organizer
      第45回 日本分子生物学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 局所的・全身的なサイトカイニンシグナルの同時制御による高成長性植物の開発2022

    • Author(s)
      蜂谷卓士
    • Organizer
      第95回日本生化学会大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナにおける硝酸還元反応低下時の呼吸鎖末端酸化酵素AOXの生理機能の解析2022

    • Author(s)
      乙丸 大輔, 押井 夏海, 鈴木孝征, 中川 強, 蜂谷 卓士
    • Organizer
      日本植物学会 第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 操作実験とオミクス解析による植物の栄養応答の研究2022

    • Author(s)
      蜂谷卓士
    • Organizer
      令和3年度生物資源科学部研究セミナー(第41回
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 硝酸還元と呼吸鎖の相互作用に関する解析2021

    • Author(s)
      蜂谷卓士
    • Organizer
      植物の栄養研究会 第 6 回交流会 2021年11月13日
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] A cellophane-supported Arabidopsis culture for seamless transfer between different media is useful for analyzing various nitrogen responses2021

    • Author(s)
      蜂谷卓士, 大屋卓博, 門田宏太, 永江杏実, 中川 強
    • Organizer
      日本土壌肥料学会2021年度北海道大会 2021年9月14日
    • Related Report
      2021 Research-status Report
  • [Presentation] 根特異的なサイトカイニン受容の低下が地上部のトランスゼアチン型サイトカイニンとバイオマス を増加させる2021

    • Author(s)
      門田宏太, 小嶋美紀子, 竹林裕美子, 鈴木孝征, 中川強, 榊原均, 蜂谷卓士
    • Organizer
      日本植物学会 第85回大会 2021年9月18日
    • Related Report
      2021 Research-status Report
  • [Presentation] シロイヌナズナにおける根特異的なサイトカイニンシグナルの欠損が地上部のトランスクリプトームに与える影響の解析2021

    • Author(s)
      門田宏太、鈴木孝征、中川強、蜂谷卓士
    • Organizer
      日本植物生理学会年会(松江)
    • Related Report
      2020 Research-status Report
  • [Remarks] 貧栄養環境で植物のバイオマスを増加させる新規技術開発に成功

    • URL

      https://www.shimane-u.ac.jp/docs/2022071200019/file_contents/20220712_shimane_university.pdf

    • Related Report
      2022 Annual Research Report
  • [Remarks] 植物の地下での情報のやりとりを発見 ~地下茎で繋がった植物株間でのコミュニケーション~

    • URL

      https://www.shimane-u.ac.jp/docs/2022020200015/file_contents/20220204.pdf

    • Related Report
      2022 Annual Research Report
  • [Remarks] 名古屋大学 研究大学強化促進事業・最先端国際研究ユニット 最先端情報分子・植物最適行動統御ユニット

    • URL

      https://www.agr.nagoya-u.ac.jp/~ck/b1/index.html#member

    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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