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Analysis of the response mechanism for fruiting body induction in basidiomycetes and its effective application to mushroom production

Research Project

Project/Area Number 20K05828
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38030:Applied biochemistry-related
Research InstitutionKagawa University

Principal Investigator

Watanabe Akira  香川大学, 農学部, 教授 (90325324)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords担子菌 / 子実体形成 / 遺伝子発現 / ラッカーゼ / 酵素機能 / キノコ
Outline of Research at the Start

担子菌において子実体(キノコ)は、温度、光、そして栄養飢餓などの外的刺激に応答して形成される。また、子実体は食用担子菌においては可食部となるなど、産業上重要な器官であるにもかかわらず、その形成の分子メカニズムについては未だ不分明な状況にある。そこで本研究では、担子菌エノキタケの子実体形成に必要な誘導条件に応答する酵素を切り口に、分子レベルでの網羅的解析を組み合わせ、誘導刺激を受けた菌糸がどのように子実体形成へと向かうのかを解析し、得られた成果のキノコ栽培への活用についても検討を行う。

Outline of Final Research Achievements

In this study, we constructed a heterologous expression system for the laccase isozyme in response to low temperature, which is significant in the fruiting body formation of Flammulina velutipes (enokitake), and analyzed various properties of the prepared enzyme. In addition, overexpression of this isozyme in F.velutipes suggested that the enzyme contributes to the growth enhancement of mycelial growth and fruiting body formation. Furthermore, information on orthologs that respond to fruiting body formation condition was acquired.

Academic Significance and Societal Importance of the Research Achievements

担子菌において子実体(キノコ)は、外的刺激に応答して形成され、食用担子菌においては可食部となるだけでなく、生理活性物質を含有する種もあることから有用物質の供給源ともなり得る産業上重要な器官である。よって、本研究で着目したラッカーゼアイソザイムをファクターとするエノキタケの成長促進効果や子実体形成条件に応答する遺伝子オルソログに関する情報は、さらなる解析は必要であるものの、学術的意義のみならず社会的にも重要な意義を持つものと考えられる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (9 results)

All 2024 2023 2022 2021

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Relationship between fruiting body development and extracellular laccase production in the edible mushroom Flammulina velutipes2022

    • Author(s)
      Aylin Cesur, Ryousuke Yamamoto, Yasuhiko Asada, Akira Watanabe
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 29 Pages: 101204-101204

    • DOI

      10.1016/j.bbrep.2022.101204

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production and properties of a laccase isozyme (FvLcc3) from the edible mushroom Flammulina velutipes, which is undetectable under the culture condition for fruiting body formation2022

    • Author(s)
      Aylin Cesur, Yuya Nada, Yasuhiko Asada, Akira Watanabe
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 86 Issue: 5 Pages: 624-627

    • DOI

      10.1093/bbb/zbac027

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] ゲノム編集を用いた担子菌Coprinopsis cinereaのオートファジー関連遺伝子Ccatg4破壊の取り組み2024

    • Author(s)
      北原昂希,刑部敬史,渡邉 彰
    • Organizer
      日本農芸化学会中四国支部第67回講演会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 麹菌発現系を用いて生産した担子菌Flammulina velutipes由来ラッカーゼアイソザイムの解析2023

    • Author(s)
      仁尾優太, Cesur Aylin, 須鼻浩平, 麻田恭彦, 渡邉 彰
    • Organizer
      日本農芸化学会2023年度中四国・西日本支部合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 担子菌Coprinopsis cinereaにおける栄養飢餓とオートファジーの関係2023

    • Author(s)
      北原昂希,麻田恭彦,渡邉 彰
    • Organizer
      第22回糸状菌分子生物学コンファレンス
    • Related Report
      2023 Annual Research Report
  • [Presentation] Functional analysis of laccase isozyme (FvLcc2) from the basidiomycete Flammulina velutipes produced using the Aspergillus oryzae heterologous expression system2023

    • Author(s)
      Yuta Nio, Aylin Cesur, Kohei Subana, Yasuhiko Asada, Akira Watanabe
    • Organizer
      Kagawa International Forum on Advanced Genomics: Environmental and Resource Genomics and Life Sciences-Ⅱ
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 担子菌Flammulina velutipes(エノキタケ)の子実体形成について ~ラッカーゼ発現との関係~2022

    • Author(s)
      渡邉 彰
    • Organizer
      第468回ビタミンB研究協議会
    • Related Report
      2022 Research-status Report
  • [Presentation] Physiological investigation of laccase isozyme in Flammulina velutipes2021

    • Author(s)
      Aylin Cesur, Kohei Subana, Ikuto Goma, Yasuhiko Asada, Akira Watanabe
    • Organizer
      第62回 日本生化学会 中国・四国支部例会
    • Related Report
      2021 Research-status Report
  • [Presentation] 担子菌類の子実体形成機構の解析2021

    • Author(s)
      渡邉 彰
    • Organizer
      第465回ビタミンB研究協議会
    • Related Report
      2021 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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