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Challenging to resolve the paradox in anthocyanin function: Study on the interactions between anthocyanin and gut microbiome

Research Project

Project/Area Number 20K05928
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionKagoshima University

Principal Investigator

HOU De-Xing  鹿児島大学, 農水産獣医学域農学系, 教授 (90305160)

Co-Investigator(Kenkyū-buntansha) 坂尾 こず枝  鹿児島大学, 農水産獣医学域農学系, 准教授 (40713285)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsアントシアニン / アントシアニン代謝産物 / 非アルコール性脂肪肝 / 脂質異常症 / 腸内細菌叢 / 酸化損傷 / 脂質異常 / 炎症 / 相互作用検討 / 機能性解明
Outline of Research at the Start

アントシアニンは、多彩な機能が知られている一方、生体への移行率は極めて低いと報告されてきた。アントシアニンの低生体利用率と高機能性は矛盾しており、いわゆるアントシアニンパラドックスな状況になっている。本研究はそのパラドックスを解明するために、腸内細菌叢を標的にし、①腸内細菌によるアントシアニンの分解及びその分解産物の機能、②アントシアニンによる腸内細菌叢及び腸粘膜等の腸内環境状況への影響、という相互作用について、動物実験を用いた代謝産物のメタボローム解析や腸内細菌叢の16S RNA解析によって明らかにする。

Outline of Final Research Achievements

The absorption rate of anthocyanin in the stomach and small intestine is too much low to explain its functionality in the living body. The purpose of this study was challenged to elucidate the contradiction between their low bioavailability and high functionality by investigating the interaction between anthocyanins and intestinal bacteria. Mouse experiments were conducted in accordance with the Kagoshima University Animal Experiment Regulations. Five-week-old mice were fed a standard diet and Westernized diet, or blueberry anthocyanin extract (BA) and its major metabolites, PGA and PCA, for 3 months. The results revealed that anthocyanins were mainly degraded into PCA and PGA in the large intestine, and then regulated intestinal bacteria and their networks related to lipid metabolism, and finally improved lipid metabolism abnormalities and disturbances in intestinal flora caused by WD.

Academic Significance and Societal Importance of the Research Achievements

アントシアニンは胃・小腸での吸収率は極めて低く、生体での機能性を説明できない。本研究はアントシアニンと腸内細菌の相互作用を解析し、その矛盾を解明することを目的にした。その結果、アントシアニンは主に大腸内でPCやPG等へ分解され、脂質代謝等に関する腸内細菌及びそのネットワークを調節し、過剰栄養による脂質代謝異常及び腸細菌叢の乱れ等を改善させた。これらの成果は、アントシアニンの低生体利用率と高機能性の矛盾解明に説明できる科学根拠を提示したものであり、アントシアニン食材を今後の食卓への健康利用に促進する社会的意義が大きい。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (5 results)

All 2023 2022 2020

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Ameliorative Effects of Anthocyanin Metabolites on Western Diet-Induced NAFLD by Modulating Co-Occurrence Networks of Gut Microbiome2023

    • Author(s)
      Nakano Hironobu、Sakao Kozue、Wada Koji、Hou De-Xing
    • Journal Title

      Microorganisms

      Volume: 11 Issue: 10 Pages: 2408-2408

    • DOI

      10.3390/microorganisms11102408

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bilberry Anthocyanins Ameliorate NAFLD by Improving Dyslipidemia and Gut Microbiome Dysbiosis2020

    • Author(s)
      Nakano Hironobu、Wu Shusong、Sakao Kozue、Hara Taichi、He Jianhua、Garcia Santos、Shetty Kalidas、Hou De-Xing
    • Journal Title

      Nutrients

      Volume: 12 Issue: 11 Pages: 3252-3252

    • DOI

      10.3390/nu12113252

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Anthocyanins food innovation: from laboratory bench to healthy dining table2023

    • Author(s)
      De-Xing HOU
    • Organizer
      EAFTA2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Innovation of anthocyanin food from laboratory bench to healthy dining table2023

    • Author(s)
      De-Xing HOU
    • Organizer
      JAACT2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 非アルコール性脂肪性肝疾患(NAFLD)におけるビルベリー及びアントシアニン代謝産物による予防効果:腸内細菌叢の視点から2022

    • Author(s)
      中野 拓宜・坂尾こず枝・侯 徳興
    • Organizer
      第76回 日本栄養・食糧学会大会
    • Related Report
      2022 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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