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Metabolite dynamics among different organs in crop under heterogeneous water environment

Research Project

Project/Area Number 20K05997
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39020:Crop production science-related
Research InstitutionNagoya University

Principal Investigator

Kano-Nakata Mana  名古屋大学, 農学国際教育研究センター, 助教 (70623958)

Co-Investigator(Kenkyū-buntansha) 若山 正隆  愛媛大学, 医学系研究科, 講師 (20721913)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords水ストレス / イネ / メタボローム解析 / 根 / フェノタイピング / 水分変動 / 土壌水分変動 / 代謝産物 / 窒素形態 / フェノタイピングシステム / 根系
Outline of Research at the Start

作物の主要な生産性規定要因である水ストレスを、時間的・空間的水分不均一性に着目し、水ストレス適応性向上に関連する代謝物の同定ならびに遺伝制御について明らかにすることを目的とする。作物の器官間とくに根系の構成根別の代謝物の変動とそれらの遺伝変異ならびに発現遺伝子の網羅的な解析を行う。あわせて低コストでハイスループットなフェノタイピング手法の確立を目指す。本研究の成果より、作物の水ストレス適応性指標となるバイオマーカーの探索やフェノタイピング技術の開発に結びつく、今後の根系育種を発展させる有用な知見の提供に繋がることが期待される。

Outline of Final Research Achievements

Water stress is one of the major constraints to crop production and roots are the first organs detecting the water stress and showing morphological changes. Since root system development is closely related to the sugar metabolism, we focused on metabolite dynamics among different organs in crop plants. The objective of this study was to identify metabolites and root phenotypes which associated with plant adaptation to water stress focusing on moisture fluctuations in rice. Metabolome analysis revealed that genotypic variation was observed in metabolite recovery (such as Glu, Gln, Asp and Asn) during re-watering after drought, which was associated with lateral root development. In addition, non-destructive phenotyping system was constructed to monitor the root system development under moisture fluctuation conditions.

Academic Significance and Societal Importance of the Research Achievements

作物の水ストレス適応性向上に関連する代謝産物や表現型の同定により、それらを水ストレス適応性指標として同ストレス条件下の収量向上に貢献できる。とくに本研究で抽出された代謝産物や表現型、それらの評価システムは、水ストレスとして焦点を当てた水分変動が頻繁に起こっていると想定される、天水栽培(灌漑設備が整っておらず自然の降雨に依存して栽培)や節水栽培に適したイネの選抜や育種にも応用することができると考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (17 results)

All 2023 2022 2021 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (13 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results) Remarks (1 results)

  • [Int'l Joint Research] Philippine Rice Research Institute/Central Luzon State University(フィリピン)

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Root system characteristics under different water regimes in three cereal species2021

    • Author(s)
      Ueda, N., Mitsuya, S., Yamauchi, A., Cabral, M.C.J., Kano-Nakata, M.
    • Journal Title

      Plant Root

      Volume: 15 Issue: 0 Pages: 10-18

    • DOI

      10.3117/plantroot.15.10

    • NAID

      130008025942

    • ISSN
      1881-6754
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Morpho-physiological and molecular mechanisms of phenotypic root plasticity for rice adaptation to water stress conditions2021

    • Author(s)
      Lucob-Agustin, N., Kawai, T., Kano-Nakata, M., Suralta, R. R., Niones, J. M., Hasegawa, T., Inari-Ikeda, M., Yamauchi, A. and Inukai, Y.
    • Journal Title

      Breeding Science

      Volume: 71 Issue: 1 Pages: 20-29

    • DOI

      10.1270/jsbbs.20106

    • NAID

      130007991312

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 作物の生理形態特性からみるプラズマ照射後の環境ストレス応答2023

    • Author(s)
      仲田(狩野)麻奈, Yap Seong Ling, Nikolay Britun, 石川健治, 橋爪博司, 堀勝, 江原宏
    • Organizer
      プラズマバイオコンソーシアム プラズマ種子科学研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Drought tolerance screening and mutation breeding of specialty rice for rainfed lowland ecosystem2023

    • Author(s)
      Agustin Ace Mugssy Lina, Natividad Marie Bie S., Ordonio Jeremias L., Cruz Pompe C. Sta., Lucob-Agustin Nonawin B., Suralta Roel R., Ehara Hiroshi, Kano-Nakata Mana
    • Organizer
      The 252th Annual Meeting of Crop Science Society of Japan
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 乾燥ストレス後の再潅水がイネ器官間の代謝変動に与える影響2022

    • Author(s)
      仲田(狩野)麻奈, 若山正隆, 門脇里恵, Deshabandu KHST, 山崎竜太朗, 三屋史朗, 山内章, 江原宏
    • Organizer
      日本作物学会第254回講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Strategy of adaptation and plasticity of crop root under water stress environment2022

    • Author(s)
      Kano-Nakata M, Yamauchi A., Inukai, Y, Hiroshi Ehara
    • Organizer
      The 11th Global Plasma Forum
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ソーラー灌水による節水効果とイネ収量性評価2022

    • Author(s)
      野田康貴, 江原宏, 仲田(狩野)麻奈
    • Organizer
      日本作物学会第253回講演会
    • Related Report
      2021 Research-status Report
  • [Presentation] Root water uptake ability in relation to developmental plasticity and hydraulic conductivity under various types of water stresses2021

    • Author(s)
      Akira Yamauchi, Mana Kano-Nakata, Shiro Mitsuya, Yoshiaki Inukai, Yumika Watanabe, Kaho Inoguchi, Roel Suralta, Jonathan Niones
    • Organizer
      The 11th Symposium of the International Society of Root Research
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 根系形態と根呼吸に着目したイネの乾燥ストレス適応反応2021

    • Author(s)
      仲田(狩野)麻奈, 三屋史朗, 江原宏, 山内章
    • Organizer
      第53回根研究集会
    • Related Report
      2021 Research-status Report
  • [Presentation] Functional significance of roots for adaptation and productivity of crop plants grown under various environmental stresses2021

    • Author(s)
      Akira Yamauchi, Mana Kano-Nakata, Shiro Mitsuya, Yoshiaki Inukai, Roel Suralta, Jonathan Niones
    • Organizer
      10th Asian Crop Science Association Conference
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Genotypic variation in root morpho-anatomical traits of rice cultivars with high and low adaptability under multi-stress environment2021

    • Author(s)
      Maria Corazon J. Cabral, Via Ann C. Marcelo, Roel R. Suralta, Jonathan M. Niones, Antoinette S. Cruz, Hiroshi Ehara, Yoshiaki Inukai, Akira Yamauchi, Mana Kano-Nakata
    • Organizer
      10th Asian Crop Science Association Conference
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Root and leaf plasticity in response to soil moisture fluctuation in rice2021

    • Author(s)
      Yasutaka Noda, Mana Kano-Nakata, Shiro Mitsuya, Akira Yamauchi
    • Organizer
      10th Asian Crop Science Association Conference
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] イネにおける土壌水分変動条件に対して発揮する気孔形成ならびに根系発育に関わる可塑性2021

    • Author(s)
      野田康貴, 仲田麻奈, 三屋史朗, 山内章
    • Organizer
      作物学会東海支部151回講演会
    • Related Report
      2021 Research-status Report
  • [Presentation] 乾燥と塩の複合ストレス条件下におけるイネ根系反応の品種間差2021

    • Author(s)
      仲田(狩野)麻奈, Cabral Maria Corazon Julaton, Marcelo Via Ann Candelaria, 江原宏
    • Organizer
      第54回根研究集会
    • Related Report
      2021 Research-status Report
  • [Presentation] The Evaluation of Genotype by Environment (G×E) Interactions of 20 OryzaSNP panel in Relation to Dry Matter Production and Root System Development2021

    • Author(s)
      Maria Corazon J. Cabral, Roel R. Suralta, Jonathan M. Niones, Antoinette S. Cruz, Hiroshi Ehara, Yoshiaki Inukai and Mana Kano-Nakata
    • Organizer
      日本作物学会第251回講演会
    • Related Report
      2020 Research-status Report
  • [Remarks] 名古屋大学農学国際教育研究センターHP

    • URL

      https://icrea.agr.nagoya-u.ac.jp/

    • Related Report
      2022 Annual Research Report 2021 Research-status Report 2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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