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Improvement of photosynthesis by the enhancement of catalytic turnover rate of Rubisco and elucidation of structure-function relationships

Research Project

Project/Area Number 20K05998
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39020:Crop production science-related
Research InstitutionKobe University

Principal Investigator

Fukayama Hiroshi  神戸大学, 農学研究科, 准教授 (60373255)

Co-Investigator(Kenkyū-buntansha) 松村 浩由  立命館大学, 生命科学部, 教授 (30324809)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords光合成 / Rubisco / C4植物 / X線結晶構造 / イネ / 酵素特性 / ソルガム / タンパク質構造 / 遺伝子組換え / ルビスコ / X線結晶構造解析 / アミノ酸置換 / RbcS / 戻し交配 / 高CO2 / 結晶構造
Outline of Research at the Start

光合成速度は収量の重要な決定因子であり,そのボトルネックは触媒速度が遅いRubiscoである.CO2濃縮回路を持つC4植物のRubiscoはC3植物の2倍以上の触媒速度を示し効率よく光合成を行っている.我々は,触媒速度の高いC4植物のRubiscoの小サブユニット(RbcS)をC3植物のイネに導入することで,イネRubiscoの触媒速度を大幅に増加させることに成功した.本研究では,それら高活性型のハイブリッドRubiscoを利用して高い光合成能力と生産性を発揮できるイネの開発を行う.また,触媒速度の高いRbcSがRubisco触媒速度を増加させるメカニズムをX線結晶構造解析により明らかにする.

Outline of Final Research Achievements

Backcrossing of CSS lines expressing hybrid Rubisco composed of sorghum RbcS and rice RbcL, did not restore seed fertility. Transgenic rice plants expressing sorghum RbcS L102I and rice RbcS I102L mutant RbcS were produced and the Rubisco activity was measured. The catalytic rate of Rubisco was significantly lower in sorghum L102I line than in CSS line, and the Kc tended to be higher in rice I102L line. We produced rice plants expressing a complete hybrid Rubisco of napier grass RbcS and rice RbcL, but this Rubisco could not function normally. X-ray crystallographic analysis of hybrid Rubisco containing OsRbcS1 revealed a previously unreported difference in the structure of the catalytic site.

Academic Significance and Societal Importance of the Research Achievements

Rubiscoは光合成速度の主要な律速因子である.本研究ではソルガムRbcSをイネに導入することで,イネに高活性型のRubiscoを付与することに成功した.また,ソルガムRbcSの102番目のロイシンが触媒速度に関係することを点突然変異を導入したRbcSを用いて明らかにした.また,102番目がイソロイシンであるOsRbcS1にも触媒速度を高める効果があるが,そのメカニズムについてもX線結晶構造解析から推定することに成功した.これらの成果は学術的な価値だけでなく,光合成の改良による作物生産性の改良に繋がる重要な成果である.

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (17 results)

All 2023 2022 2021 2020 Other

All Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] Deepwater response in the African cultivated rice Oryza glaberrima2023

    • Author(s)
      Quanshu Luo, Daisuke Sasayama, Misaki Nakazawa, Tomoko Hatanaka, Hiroshi Fukayama, Tetsushi Azuma
    • Journal Title

      Plant Production Science

      Volume: 26 Issue: 1 Pages: 65-75

    • DOI

      10.1080/1343943x.2022.2161401

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the Functions of Starch Synthase IIIb Expressed in the Vegetative Organs of Rice (<i>Oryza sativa</i>L.)2022

    • Author(s)
      Morita Ryutaro、Crofts Naoko、Miura Satoko、Ikeda Ken-ichi、Aoki Naohiro、Fukayama Hiroshi、Fujita Naoko
    • Journal Title

      Plant and Cell Physiology

      Volume: 64 Issue: 1 Pages: 94-106

    • DOI

      10.1093/pcp/pcac143

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adaptive responses to flood in wild rice species with various genomes other than AA2022

    • Author(s)
      Sasayama D., Niikawa M., Hatanaka T., Fukayama H. and Azuma T.
    • Journal Title

      Plant Production Science

      Volume: 25 Issue: 3 Pages: 350-358

    • DOI

      10.1080/1343943x.2022.2073896

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Internodal elongation under submergence in <i>Oryza sativa</i>/<i>Oryza glumaepatula</i> introgression lines2022

    • Author(s)
      Sasayama D., Yuan S., Hatanaka T., Fukayama H. and Azuma T.
    • Journal Title

      Journal of Crop Research

      Volume: 67 Issue: 0 Pages: 35-40

    • DOI

      10.18964/jcr.67.0_35

    • ISSN
      1882-885X, 2424-1318
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of growth direction in crown roots in rice germinated seedlings by light2022

    • Author(s)
      Matsumoto K., Sasayama D., Hatanaka T., Fukayama H. and Azuma T.
    • Journal Title

      Journal of Crop Research

      Volume: 67 Issue: 0 Pages: 51-55

    • DOI

      10.18964/jcr.67.0_51

    • ISSN
      1882-885X, 2424-1318
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Different DGAT1s vary widely in function and a targeted amino acid substitution enhances oil accumulation2022

    • Author(s)
      Tomoko Hatanaka, Yoshiki Tomita, Daisuke Matsuoka, Daisuke Sasayama, Hiroshi Fukayama, Tetsushi Azuma, Mohammad Fazel Soltani Gishini, and David Hildebrand
    • Journal Title

      Journal of Experimental Botany

      Volume: 73 Issue: 9 Pages: 3030-3043

    • DOI

      10.1093/jxb/erac084

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] CO2-Responsive CCT Protein interacts with 14-3-3 proteins and controls the expression of starch synthesis-related genes2021

    • Author(s)
      Fukayama H., Miyagawa H., Shibatani N., Koudou A., Yamauchi Y., Matsuoka D., Sasayama D., Hatanaka T., Azuma T., Morita R.
    • Journal Title

      Plant Cell Environment

      Volume: 44 Issue: 8 Pages: 2480-2493

    • DOI

      10.1111/pce.14084

    • NAID

      120007124109

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Overexpression of both Rubisco and Rubisco activase rescues rice photosynthesis and biomass under heat stress2021

    • Author(s)
      Qu Yuchen、Sakoda Kazuma、Fukayama Hiroshi、Kondo Eri、Suzuki Yuji、Makino Amane、Terashima Ichiro、Yamori Wataru
    • Journal Title

      Plant, Cell & Environment

      Volume: 44 Issue: 7 Pages: 1-13

    • DOI

      10.1111/pce.14051

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rubiscoの高活性化によるイネの光合成能力と生産性の改良2021

    • Author(s)
      深山浩,松村浩由
    • Journal Title

      光合成研究

      Volume: 31 Pages: 110-119

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of Introduction of Sorghum RbcS with Rice RbcS Knockdown by RNAi on Photosynthetic Activity and Dry Weight in Rice.2021

    • Author(s)
      Kazuma Sakoda , Akito Yamamoto , Chie Ishikawa , Yojiro Taniguchi , Hiroyoshi Matsumura & Hiroshi Fukayama
    • Journal Title

      Plant Production Science

      Volume: in press Issue: 3 Pages: 1-8

    • DOI

      10.1080/1343943x.2020.1847669

    • NAID

      120007125729

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4-Plant-like High Catalytic Activity2020

    • Author(s)
      Hiroyoshi Matsumura, Keita Shiomi, Akito Yamamoto, Yuri Taketani, Noriyuki Kobayashi, Takuya Yoshizawa, Shun-ichi Tanaka, Hiroki Yoshikawa, Masaki Endo, Hiroshi Fukayama,
    • Journal Title

      Molecular Plant

      Volume: 13 Issue: 11 Pages: 1570-1581

    • DOI

      10.1016/j.molp.2020.08.012

    • NAID

      120006919994

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] イネにおいてCRCTが制御するデンプン合成関連遺伝子の糖応答性の品種間差の解析2023

    • Author(s)
      宮川文宏・森田隆太郎・笹山大輔・畠中知子・東哲司・深山浩
    • Organizer
      日本作物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 竹谷友里・大河世生・塩見慶太・笹山大輔・畠中知子・東哲司・深山浩2023

    • Author(s)
      Rubiscoの高活性化に関係する可能性のあるC4植物型RbcSの ロイシン102の酵素特性への効果
    • Organizer
      日本作物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] RubiscoとRubisco activaseの二重過剰発現は高温環境におけるイネの光合成と生産性を向上させる2022

    • Author(s)
      曲玉辰,迫田和馬,深山浩,寺島一郎,矢守航
    • Organizer
      日本作物学会
    • Related Report
      2021 Research-status Report
  • [Presentation] A Challenge for the Improvement of Photosynthetic Capacity by the Introduction of C4-Like Rubisco in Rice2021

    • Author(s)
      Hiroshi Fukayama, Keita Shiomi, Hiroki Yoshikawa, Daisuke Sasayama, Tomoko Hatanaka, Tetsushi Azuma, Takuya Yoshizawa, Shun-ichi Tanaka, Hiroyoshi Matsumura
    • Organizer
      アジア作物学会
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] イネ葉鞘におけるCRCTによるデンプン合成関連遺伝子の発現調節とその品種間差2021

    • Author(s)
      宮川文宏・柴谷直幹,高堂愛子,笹山大輔,畠中知子,東哲司,森田隆太郎,深山 浩
    • Organizer
      近畿作物育種研究会
    • Related Report
      2021 Research-status Report
  • [Remarks] 光合成におけるCO2固定酵素Rubiscoの触媒活性の改良に成功

    • URL

      https://www.kobe-u.ac.jp/research_at_kobe/NEWS/news/2020_08_31_01.html

    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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