• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of a hydrophobic-hydrophilic crown-like structure in aquatic insects under the water surface

Research Project

Project/Area Number 20K06071
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39050:Insect science-related
Research InstitutionTokyo University of Agriculture (2023)
Hamamatsu University School of Medicine (2020-2022)

Principal Investigator

Takaku Yasuharu  東京農業大学, 農学部, 教授 (60378700)

Co-Investigator(Kenkyū-buntansha) 針山 孝彦  浜松医科大学, 光尖端医学教育研究センター, 特命研究教授 (30165039)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords電子顕微鏡 / 高真空 / 水性生物 / NanoSuit / 水生昆虫 / 濡れ性 / 疎水性 / 親水性 / NanoSuit法 / 昆虫 / 微細構造 / 水面直下
Outline of Research at the Start

水を界面とする昆虫の、体表の疎水性・親水性について多くの研究がなされている。しかし、水面直下にのみニッチをもつ生物が、どのような機構で棲息しているのかの研究は皆無である。申請者らは、水面直下で生活する生物に注目し「超疎水性-超親水性の複合構造」により水面を効果的に利用していることを発見した。本研究では、1) 生きたまま・濡れたままの電子顕微鏡観察できる新技術 (NanoSuit法) 、2) 高速度撮影による運動解析法、3) 電気生理学的手法などを用いて、この「複合構造」がもつ未知の機構・機能を解明することで、昆虫の新たな生理・生態学を構築すると共に、生物に学んだ新素材開発につなげる。

Outline of Final Research Achievements

Various insects utilize hydrophobic biological surfaces to live on the water surface, while other organisms possess hydrophilic properties to live within the water column. Dixidae Larvae reside just below the water surface and never submerge. However, little is known about how the larvae live in such an ecological niche. In this study, we reveal their remarkable features and elucidate the mechanisms by the morphological analysis using the NanoSuit method, and the movement analysis using the high-speed photography. We find that a complex ‘crown’ structure on its abdomen consists of super-hydrophobic and super-hydrophilic structures. Our results demonstrate that the combination of these conflicting properties enables the larvae to position itself and to move just under the water surface: the hydrophobic region utilizes the water’s surface tension to function as an ‘adhesive disc’, and the hydrophilic region behaves as a ‘rudder’ during locomotion.

Academic Significance and Societal Importance of the Research Achievements

水面直下の生物に注目した研究は国内外ともに無かった。さらにNanoSuit 法により、生きたまま生物試料を高分解能観察する技術は、これまでの観察結果を正確にするだけでなく、生命現象そのものについて新たな超微形態的知見を与えることは明白であり、注目昆虫および解析法の両者ともに学術的意義がある。また、本研究による新規知見は、生命科学全体に新たな視点を与え、農学・生物学・医学などの生命科学分野にこれまでにない新機構を提案するとともに、バイオミメティクス研究の推進にも貢献できる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (20 results)

All 2024 2023 2022 2021 2020

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 8 results) Presentation (9 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Book (2 results)

  • [Journal Article] Route of pesticide spread on the body surface of Blattella germanica (Linnaeus): a NanoSuit-energy dispersive X-ray spectroscopy analysis2023

    • Author(s)
      Takaku Yasuharu、Shiraki Katsumi、Suzuki Chiaki、Takehara Sayuri、Nishii Hiroyuki、Sasaki Tomonori、Hariyama Takahiko
    • Journal Title

      Scientific Reports

      Volume: 13(1) Issue: 1

    • DOI

      10.1038/s41598-023-41474-x

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microscopy and biomimetics: the NanoSuit; method and image retrieval platform2022

    • Author(s)
      Hariyama T, Takaku Y, Kawasaki H, Shimomura M, Senoh C, Yamahama Y, Hozumi A, Ito S, Matsuda N, Yamada S, Itoh T, Haseyama M, Ogawa T, Mori N, So S, Mitsuno H, Ohara M, Nomura S, Hirasaka M
    • Journal Title

      Microscopy

      Volume: 71(1) Issue: suppl 1 Pages: 1-12

    • DOI

      10.1093/jmicro/dfu042

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microscopy and biomimetics: the NanoSuit? method and image retrieval platform2021

    • Author(s)
      Hariyama Takahiko、Takaku Yasuharu、Kawasaki Hideya、Shimomura Masatsugu、Senoh Chiyo、Yamahama Yumi、Hozumi Atsushi、Ito Satoru、Matsuda Naoto、Yamada Satoshi、Itoh Toshiya、Haseyama Miki、Ogawa Takahiro、Mori Naoki、So Shuhei、Mitsuno Hidefumi、Ohara Masahiro、Nomura Shuhei、Hirasaka Masao
    • Journal Title

      Microscopy

      Volume: 71 Issue: 1 Pages: 1-12

    • DOI

      10.1093/jmicro/dfab042

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hydrophobic-hydrophilic crown-like structure enables aquatic insects to reside effectively beneath the water surface2021

    • Author(s)
      Suzuki Chiaki、Takaku Yasuharu、Suzuki Hiroshi、Ishii Daisuke、Shimozawa Tateo、Nomura Shuhei、Shimomura Masatsugu、Hariyama Takahiko
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1 Pages: 1-8

    • DOI

      10.1038/s42003-021-02228-5

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Antenna cleaning is essential for precise behavioral response to the alarm and nestmate-non-nestmate discrimination pheromones in Japanese carpenter ant (Camponotus japonicus)2021

    • Author(s)
      Mizutani H, Tagai K, Habe S, Takaku Y, Uebi T, Kimura T, Hariyama T, Ozaki M
    • Journal Title

      Insects

      Volume: 12(9) Issue: 9 Pages: 773-773

    • DOI

      10.3390/insects12090773

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hydrophobic-hydrophilic crown-like structure enables aquatic insects to reside effectively beneath the water surface2021

    • Author(s)
      Suzuki C, Takaku Y(共筆頭著者), Suzuki H, Ishii D, Shimozawa T, Nomura S, Shimomura M, Hariyama T
    • Journal Title

      Commun Biol

      Volume: In press

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Living Organisms under an Electron Microscope: the NanoSuit® Method aiming for Medical and Industrial Applications2020

    • Author(s)
      Takahiko Hariyama, Yasuharu Takaku, Chiyo Senoh, Satoshi Yamada, Toshiya Itoh, Chiaki Suzuki, Sayuri Takehara, Satoshi Hirakawa, Hideya Kawasaki
    • Journal Title

      Journal of Photopolymer Science and Technology

      Volume: 33 Issue: 5 Pages: 517-522

    • DOI

      10.2494/photopolymer.33.517

    • NAID

      130007867678

    • ISSN
      0914-9244, 1349-6336
    • Year and Date
      2020-07-01
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Imaging dataset of fresh hydrous plants obtained by field-emission scanning electron microscopy conducted using a protective NanoSuit.2020

    • Author(s)
      Takehara S, Takaku Y(共筆頭著者), Shimomura M, Hariyama T
    • Journal Title

      PLOS ONE

      Volume: 15(5) Issue: 5 Pages: e0232992-e0232992

    • DOI

      10.1371/journal.pone.0232992

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] In situ elemental analysis of living biological specimens using ‘NanoSuit’ and EDS methods in FE-SEM2020

    • Author(s)
      Takaku Y, Takehara S, Suzuki C, Suzuki H, Shimomura M, Hariyama T
    • Journal Title

      Sci Rep

      Volume: 10(1) Issue: 1

    • DOI

      10.1038/s41598-020-71523-8

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 黒色を呈する昆虫の赤外線反射機能2024

    • Author(s)
      山口 泰生, 高久 康春
    • Organizer
      日本昆虫学会第84回大会・第68回日本応用動物昆虫学会大会 合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] NanoSuit method for the observation of living/hydrous organisms in an SEM2022

    • Author(s)
      Yasuharu Takaku
    • Organizer
      Microscopy & Microanalysis, 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 昆虫体表面における殺虫剤の広がり:NanoSuit-EDS法による解析2022

    • Author(s)
      高久 康春, 白木 克実, 鈴木 千晶, 竹原 さゆり, 西井 博行, 佐々木 智基, 針山 孝彦
    • Organizer
      第71回高分子討論会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Characterization of ultrastructural morphology of human sperms by field-emission scanning electron microscopy using the NanoSuit method2021

    • Author(s)
      So S, Takaku Y, Ohta I, Tawara F, Hariyama T
    • Organizer
      European Society of Human Reproduction and Embryology (ESHRE)’s 37th Annual Meeting
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] NanoSuit method for the observation of living/wet organisms in an SEM2021

    • Author(s)
      Takaku Y
    • Organizer
      BioEM Talks 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] NanoSuit method for the observation of living/wet organisms in an SEM2021

    • Author(s)
      Takaku Y
    • Organizer
      Microscopy & Microanalysis
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] NanoSuit法を用いたFE-SEMによる精子超微形態の特徴づけ2021

    • Author(s)
      宗 修平, 高久 康春, 太田 勲, 俵 史子, 針山 孝彦
    • Organizer
      日本アンドロロジー学会第40回学術大会
    • Related Report
      2021 Research-status Report
  • [Presentation] NanoSuit法を用いたFE-SEMによる精子超微形態観察法の確立2020

    • Author(s)
      宗 修平, 高久 康春(共筆頭著者), 太田 勲, 俵 史子, 針山 孝彦
    • Organizer
      第38回日本受精着床学会総会・学術講演会
    • Related Report
      2020 Research-status Report
  • [Presentation] NanoSuit法を用いたFE-SEMによる精子超微形態観察法の確立2020

    • Author(s)
      宗 修平, 俵 史子, 高久 康春
    • Organizer
      第65回日本生殖医学会学術講演会・総会
    • Related Report
      2020 Research-status Report
  • [Book] バイオミメティクス―持続可能な社会へ導く技術革新のヒント―2021

    • Author(s)
      高久 康春, 針山 孝彦, 河崎 秀陽
    • Total Pages
      367
    • Publisher
      株式会社シーエムシー出版
    • ISBN
      9784781315638
    • Related Report
      2021 Research-status Report 2020 Research-status Report
  • [Book] 電子顕微鏡で観る昆虫: NanoSuit法によるそのままの生物表面解析2020

    • Author(s)
      針山 孝彦, 河崎 秀陽, 高久 康春
    • Total Pages
      11
    • Publisher
      昆蟲 (ニューシリーズ)
    • Related Report
      2020 Research-status Report

URL: 

Published: 2020-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi