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Behavior of antimicrobial peptides in kuruma shrimp during antiviral response

Research Project

Project/Area Number 20K06247
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionOkayama University of Science (2022)
Fisheries Research and Education Agency (2020-2021)

Principal Investigator

Mekata Tohru  岡山理科大学, 獣医学部, 准教授 (40597944)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords抗微生物性ペプチド / クルマエビ / ウイルス感染 / 抗ウイルス応答 / 抗LPS因子 / 抗微生物性ペプチド群 / 免疫応答 / 血清プロテオーム
Outline of Research at the Start

抗ウイルス応答に関連する抗微生物性ペプチド群の遺伝子同定し、これらの測定系を構築する。病原体感染時におけるこれらの遺伝子の挙動あるいは局在を明らかにする。
また、これらの抗微生物性ペプチド群の産生誘導機構に関与する遺伝子群を特定するために、当該因子投与時の網羅的な遺伝子発現を解析する。
発現に変動が見られた遺伝子を抑制後にペプチド群の産生量を測定し,産生機構に関連する遺伝子を特定する。

Outline of Final Research Achievements

In order to understand the behavior of antimicrobial peptides among the factors related to the antiviral response in the hemolymph of shrimp, the identification of these factors and the construction of a detection system were conducted. After collecting the hemolymph from shrimp that had induced an antiviral response and removing Highly Abundant Proteins by ultrafiltration column, the detection of anti-microbial peptides was attempted by mass spectrometry and genetic analysis. The analysis revealed that C-type lysozyme and several anti-LPS factors were detected in the hemolymph. Furthermore, the expression levels of all factors were increased during antiviral response, suggesting that these factors play an important role during viral infection.

Academic Significance and Societal Importance of the Research Achievements

エビ類の養殖では様々なウイルス病が蔓延しており、一刻も早い防除対策が世界的に望まれている。このような疾病の被害を未然に防ぐために、免疫賦活剤や感染防除対策に関する研究開発が行われている。このような研究の中で、エビ類の抗ウイルス免疫能を向上させる物質が発見されてはいるが、その作用機序は不明なままである。このような免疫賦活剤の実用化を進めていく上でも、その作用機序の解明が望まれている。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (8 results)

All 2023 2022 2021 2020

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (3 results)

  • [Journal Article] Immunostimulation of shrimp through oral administration of silkworm pupae expressing VP15 against WSSV2022

    • Author(s)
      Boonyakida Jirayu、Nakanishi Takafumi、Satoh Jun、Shimahara Yoshiko、Mekata Tohru、Park Enoch Y.
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 128 Pages: 157-167

    • DOI

      10.1016/j.fsi.2022.07.043

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Strategy for understanding the biological defense mechanism involved in immune priming in kuruma shrimp2021

    • Author(s)
      Mekata Tohru
    • Journal Title

      Developmental & Comparative Immunology

      Volume: 125 Pages: 104228-104228

    • DOI

      10.1016/j.dci.2021.104228

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus2021

    • Author(s)
      Boonyakida Jirayu、Xu Jian、Satoh Jun、Nakanishi Takafumi、Mekata Tohru、Kato Tatsuya、Park Enoch Y.
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 12766-12766

    • DOI

      10.1038/s41598-021-92002-8

    • NAID

      120007126510

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Immune toxicity of phenanthrene and its combined effects of white spot syndrome virus on the survival of kuruma shrimp (Penaeus Japonicus)2021

    • Author(s)
      Ito Mana、Satoh Jun、Hano Takeshi、Mekata Tohru、Ito Katsutoshi
    • Journal Title

      Ecotoxicology and Environmental Safety

      Volume: 208 Pages: 111640-111640

    • DOI

      10.1016/j.ecoenv.2020.111640

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Antigenic properties of VP15 from white spot syndrome virus in kuruma shrimp Marsupenaeus japonicus.2020

    • Author(s)
      Jirayu Boonyakidaa, Jian Xub, Jun Satoh, Takafumi Nakanishi,Toru Mekata,Tatsuya Kato, Enoch Y.Park.
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 101 Pages: 152158-152158

    • DOI

      10.1016/j.fsi.2020.03.061

    • NAID

      120006821287

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] クルマエビ高成長マーカー候補遺伝子2023

    • Author(s)
      安池元重・川戸智・米加田徹・菅谷琢磨
    • Organizer
      令和5年度日本水産学会春季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Identification of Effective Peptide Derived from WSSV-VP15 for Protection of Masupenaeus Japonicus Against WSSV2021

    • Author(s)
      Jirayu Boonyakida, Jian Xu, Jun Satoh, Takafumi Nakanishi, Toru Mekata, Tatsuya Kato, and Enoch Y. Park
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2021 Research-status Report
  • [Presentation] クルマエビの免疫様現象関連因子の発現抑制によるWSSVへの抵抗性について2021

    • Author(s)
      佐藤 純・米加田徹
    • Organizer
      令和3年度日本魚病学会春季大会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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