• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of environmental water clarification-type algal cultivation unit for biomass production ~Towards the realization of a low-carbon society

Research Project

Project/Area Number 20K06326
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

Fujiwara Shoko  東京薬科大学, 生命科学部, 教授 (30266895)

Co-Investigator(Kenkyū-buntansha) 岡田 克彦  東京薬科大学, 生命科学部, 助教 (40301551)
佐藤 典裕  東京薬科大学, 生命科学部, 准教授 (50266897)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords微細藻類 / CO2固定 / 培養装置 / 環境水浄化 / バイオマス / 水質浄化 / リンの除去回収
Outline of Research at the Start

CO2問題の解決のため、光合成によるCO2固定の利用、特に生育が速く食糧生産と競合しない微細藻類によるバイオマス燃料の生産が注目されている。しかし、まだ収量とコスト面で大きな壁がある。本研究では、この壁を克服するために、当研究室で開発したユニークな固相表面連続培養系を最適化することにより、施設面積当たりの生産率が高く、日本等の温帯以北で通年培養できる培養ユニットの開発を行う。合わせて、環境水の浄化をしつつ培養を行う系を構築する。

Outline of Final Research Achievements

CO2 fixation by microalgae has recently attracted much attention to solve the CO2 problem, because they can grow faster and do not compete with agriculture. In this study, evaluation and development of an algal cell-attached solid surface culture system were performed, using Chlorella. To precisely asses the photosynthetic rate on the solid surface, we established a method for measuring the CO2 fixation rate with IRGA. PAM analysis and transcriptomic analysis by RNA-Seq suggested that cells transferred to a solid surface become stressed immediately after transfer but can recover their high photosynthetic activity through adaptation of photosynthetic machinery and metabolic flow as well as induction of general stress response mechanisms within 24 h. Furthermore, biomass production was compared between indoor and outdoor, and a portable tubular system containing an algal cell-attached solid surface for phosphorous recovery was developed.

Academic Significance and Societal Importance of the Research Achievements

植物葉で広く用いられているIRGAでCO2固定速度測定法を確立し、固相上での光合成速度を迅速かつ正確に評価することが可能になった。また、光合成特性とRNA-seqの解析から、固相表面培養で維持される高い光合成活性の分子的基盤を明らかにすることができた。さらに、無機排水からのリン回収用の可搬型固相表面連続培養装置を開発した。エタノール製造工場由来の無機排水や池の水からも短時間で大部分のリンを除去することができ、細胞の生育も維持される培養装置を作製することができた。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (29 results)

All 2024 2023 2022 2021 2020

All Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 11 results,  Open Access: 4 results) Presentation (17 results) (of which Invited: 1 results)

  • [Journal Article] Investigation of the cause of the sensitivity difference to pyrazosulfuron-ethyl between the green algae, <i>Raphidocelis subcapitata</i> and <i>Desmodesmus subspicatus</i>, focusing on target-site and nontarget-site resistance2024

    • Author(s)
      Shinpei Ohnuki、Norihiro Sato、Shoko Fujiwara
    • Journal Title

      Japanese Journal of Environmental Toxicology

      Volume: 27 Issue: 1 Pages: 9-22

    • DOI

      10.11403/jset.27.9

    • ISSN
      1344-0667, 1882-5958
    • Year and Date
      2024-04-02
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 微細藻類による二酸化炭素固定、その能力と条件2024

    • Author(s)
      都筑幹夫、岡田克彦、藤原祥子
    • Journal Title

      東京薬科大学研究紀要

      Volume: 27

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Cellular response of Parachlorella kessleri to a solid surface culture environment2023

    • Author(s)
      Miyauchi Hiroki、Ishikawa Tomoharu、Hirakawa Yutaro、Sudou Ayumu、Okada Katsuhiko、Hijikata Atsushi、Sato Norihiro、Tsuzuki Mikio、Fujiwara Shoko
    • Journal Title

      Frontiers in Plant Science

      Volume: 14 Pages: 1175080-1175080

    • DOI

      10.3389/fpls.2023.1175080

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kre6 (yeast 1,6-β-transglycosylase) homolog, PhTGS, is essential for β-glucan synthesis in the haptophyte Pleurochrysis haptonemofera2023

    • Author(s)
      Inukai Mayuka、Kobayashi Naoya、Endo Hirotoshi、Asakawa Koki、Amano Keisuke、Yasuda Yuki、Cenci Ugo、Colleoni Christophe、Ball Steven、Fujiwara Shoko
    • Journal Title

      Frontiers in Bioengineering and Biotechnology

      Volume: 11 Pages: 1259587-1259587

    • DOI

      10.3389/fbioe.2023.1259587

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biomass and Lipid Production in the Aerial Microalga Coccomyxa subellipsoidea KGU-D001 in the Liquid and Aerial Phases2023

    • Author(s)
      Aburai Nobuhiro、Kawashima Naritaka、Morita Rei、Miyauchi Hiroki、Okada Katsuhiko、Sato Norihiro、Fujiwara Shoko、Fujii Katsuhiko
    • Journal Title

      BioEnergy Research

      Volume: 16 Issue: 4 Pages: 2479-2488

    • DOI

      10.1007/s12155-023-10569-8

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Acclimation of the Aerial Microalga Coccomyxa subellipsoidea KGU-D001 to Water Stress in the Aerial Phase2023

    • Author(s)
      Aburai Nobuhiro、Morita Rei、Miyauchi Hiroki、Okada Katsuhiko、Sato Norihiro、Fujiwara Shoko、Fujii Katsuhiko
    • Journal Title

      BioEnergy Research

      Volume: 17 Issue: 1 Pages: 622-633

    • DOI

      10.1007/s12155-023-10651-1

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diacylglyceryl-N,N,N-trimethylhomoserine-dependent lipid remodeling in a green alga, Chlorella kessleri2022

    • Author(s)
      Oishi Yutaro、Otaki Rie、Iijima Yukari、Kumagai Eri、Aoki Motohide、Tsuzuki Mikio、Fujiwara Shoko、Sato Norihiro
    • Journal Title

      Communications Biology

      Volume: 5 Issue: 1 Pages: 19-19

    • DOI

      10.1038/s42003-021-02927-z

    • Related Report
      2022 Research-status Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] One of the isoamylase isoforms, CMI294C, is required for semi-amylopectin synthesis in the rhodophyte Cyanidioschyzon merolae2022

    • Author(s)
      Maeno Toshiki、Yamakawa Yuki、Takiyasu Yohei、Miyauchi Hiroki、Nakamura Yasunori、Ono Masami、Ozaki Noriaki、Utsumi Yoshinori、Cenci Ugo、Colleoni Christophe、Ball Steven、Tsuzuki Mikio、Fujiwara Shoko
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 967165-967165

    • DOI

      10.3389/fpls.2022.967165

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of an algal cell-attached solid surface culture system for simultaneous wastewater treatment and biomass production2021

    • Author(s)
      Miyauchi Hiroki、Harada Kohei、Suzuki Yoshino、Okada Katsuhiko、Aoki Motohide、Umemura Tomonari、Fujiwara Shoko、Tsuzuki Mikio
    • Journal Title

      Algal Research

      Volume: 58 Pages: 102394-102394

    • DOI

      10.1016/j.algal.2021.102394

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Requirement of the exopolyphosphatase gene for cellular acclimation to phosphorus starvation in a cyanobacterium, Synechocystis sp. PCC 68032021

    • Author(s)
      Hiyoshi Tatsunori、Oyanagi Kenta、Niki Takuma、Fujiwara Shoko、Sato Norihiro
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 540 Pages: 16-21

    • DOI

      10.1016/j.bbrc.2020.12.095

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of CO2 fixation on algal biofilms with an infrared gas analyzer and importance of a space-rich structure on the surface2020

    • Author(s)
      Miyauchi Hiroki、Okada Katsuhiko、Fujiwara Shoko、Tsuzuki Mikio
    • Journal Title

      Algal Research

      Volume: 46 Pages: 101814-101814

    • DOI

      10.1016/j.algal.2020.101814

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Energy conservation in photosynthetic microorganisms2020

    • Author(s)
      Okada, K., S. Fujiwara, M. Tsuzuki
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 66 Issue: 2 Pages: 59-65

    • DOI

      10.2323/jgam.2020.02.002

    • NAID

      130007855508

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 光合成生物におけるアシルプラストキノン類の分布2024

    • Author(s)
      伊藤 凌,遠藤 瑞季,青木 元秀,大久保 秀士,藤原 祥子,佐藤 典裕
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] クロレラのリン欠乏ストレス応答:脂質リモデリングと細胞内リン源2024

    • Author(s)
      茂木 空男,飯島 裕加里,木村 江里子,藤原 祥子,佐藤 典裕
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 固相表面培養におけるParachlorella細胞の環境応答2023

    • Author(s)
      平川 悠太郎,石川 禎治,宮内 啓喜,岡田 克彦,土方 敦司,佐藤 典裕,都筑 幹夫,藤原 祥子
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 円石藻Pleurochrysis haptonemoferaにおけるβグルカン合成関連酵素の探索2023

    • Author(s)
      安田 有輝,山本 昇吾,藤原 祥子
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ゲノム解析による石灰化関連遺伝子の解析及び探索2023

    • Author(s)
      山本 昇吾,犬飼 茉由加,鈴木 重勝,藤原 祥子
    • Organizer
      第18回バイオミネラリゼーションワークショップ
    • Related Report
      2023 Annual Research Report
  • [Presentation] 担持体に付着させたParachlorella細胞の環境応答2023

    • Author(s)
      石川禎治,平川悠太郎,宮内啓喜,岡田克彦,佐藤典裕,藤原祥子
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] 円石藻 Pleurochrysis における石灰化関連タンパク質ConC12の解析2022

    • Author(s)
      山本昇吾,犬飼茉由加,鈴木重勝,藤原祥子
    • Organizer
      第17回バイオミネラリゼーションワークショップ
    • Related Report
      2022 Research-status Report
  • [Presentation] 原始紅藻Cyanidioschyzon merolaeのイソアミラーゼ変異株の解析2022

    • Author(s)
      山川宥紀, 宮内啓喜, 前野俊樹, 中村保典, 小野雅美,尾崎紀昭, 藤原祥子
    • Organizer
      日本藻類学会第46回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Parachlorella細胞の固相表面培養における光合成特性および環境応答2021

    • Author(s)
      石川禎治, 宮内啓喜, 岡田克彦, 藤原祥子, 都筑幹夫
    • Organizer
      第21回マリンバイオテクノロジー学会
    • Related Report
      2021 Research-status Report
  • [Presentation] トレボウクシア藻綱Parachlorellaの固相表面上での光合成特性および環境応答2021

    • Author(s)
      宮内啓喜,石川禎治, 須藤歩,岡田克彦,藤原祥子, 都筑幹夫
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] クロレラの混合ストレス誘導性の油脂蓄積を支える代謝基盤・エネルギー代謝と炭素代謝遺伝子発現の制御2021

    • Author(s)
      近藤美鞠,飯島裕加里,大滝理恵,藤原祥子,佐藤典裕
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ヒ素ストレス条件下でのエネルギー代謝と脂質代謝の制御2021

    • Author(s)
      飯島裕加里,大石裕太郎,藤原祥子,佐藤典裕
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 固相表面上培養におけるトレボウクシア藻綱Parachlorella細胞の光合成特性および環境応答2021

    • Author(s)
      宮内 啓喜, 石川 禎治, 岡田 克彦, 藤原 祥子, 都筑 幹夫
    • Organizer
      日本藻類学会第45回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 固相上クロレラバイオフィルムの光合成およびリン吸収特性2020

    • Author(s)
      宮内 啓喜,原田 康平,岡田 克彦,都筑 幹夫,藤原 祥子
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 緑藻クロレラにおける核の発現ベクター構築2020

    • Author(s)
      飯島 裕加里,大石 裕太郎,大滝 理恵,藤原 祥子, 佐藤 典裕
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 原始紅藻 Cyanidioshyzon merolae のイソアミラーゼ変異株の解析2020

    • Author(s)
      山川 宥紀,藤原 祥子,中村 保典,小野 雅美,尾崎 紀昭, 宮内 啓喜,前野 俊樹
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Mysterious cell covering formation in the coccolithophorids2020

    • Author(s)
      Shoko Fujiwara
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Research-status Report
    • Invited

URL: 

Published: 2020-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi