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Structural basis for iron-stress-induced protein of cyanobacteria

Research Project

Project/Area Number 20K06502
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43020:Structural biochemistry-related
Research InstitutionYamagata University

Principal Investigator

KUBOTA-KAWAI HISAKO  山形大学, 理学部, 准教授 (10599228)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords光合成 / 光化学系 / 鉄欠乏 / 集光アンテナ / チラコイド膜 / 原子間力顕微鏡 / 光化学系I / シアノバクテリア
Outline of Research at the Start

鉄は地殻に多く含まれる元素であるが、その殆どは生物が利用できない酸化状態で存在している。このような環境に於いてシアノバクテリアではIdiA(Iron deficiency induced protein A)やisiA(iron stress induced protein A)と呼ばれる蛋白質が合成される。IdiAは光化学系II(PSII)に結合し、酸化ストレスから保護する、またIsiAは光化学系I(PSI)に結合し集光を補助するとされている。私は窒素固定シアノバクテリアを用いて、IdiAとPSII、またIsiAとPSI四量体の構造解析を行い、その機能の分子基盤を明らかにすることを目指す。

Outline of Final Research Achievements

In low-iron stress environments, cyanobacteria express IsiA (iron stress induced gene A), which donates energy to PSI core. In this study, we used atomic force microscopy (AFM) to visualize the native organization of PSI and IsiA within the thylakoid membranes from cyanobacterium Anabaena. AFM images showed that PSI complexes were densely packed in thylakoid membranes. The PSI tetramer has a protruding height of 2-3 nm above the membrane surface and possesses a characteristic cytoplasmic side structure composed of PsaC, PsaD and PsaE. The organization of IsiA around PSI remains to be further investigated.

Academic Significance and Societal Importance of the Research Achievements

現在、日本を含めた120以上の国や地域が「2050年カーボンニュートラル(温室効果ガスの排出を全体としてゼロにする)」という目標を掲げている。このような国際的潮流が加速している中、人工光合成による効率的な太陽光エネルギーの利用が求められている。
本研究ので得られた、巨大なPSI複合体をもつAnabaenaが効率よく光を集めるためにどのようにPSI四量体をチラコイド膜上に配置しているかという基礎科学的な情報は、人工光合成における光捕集効率の向上に貢献できると考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (7 results)

All 2022 2021 2020

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Conformation of Light-Harvesting Complex II Trimer Depends upon Its Binding Site2022

    • Author(s)
      Kim E, Kubota-Kawai H, Kawai F, Yokono M, Minagawa J
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 126 Issue: 31 Pages: 5855-5865

    • DOI

      10.1021/acs.jpcb.2c04061

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal structure of Tam41 cytidine diphosphate diacylglycerol synthase from a Firmicutes bacterium2021

    • Author(s)
      Kimura Keisuke、Kawai Fumihiro、Kubota-Kawai Hisako、Watanabe Yasunori、Tomii Kentaro、Kojima Rieko、Hirata Kunio、Yamamori Yu、Endo Toshiya、Tamura Yasushi
    • Journal Title

      The Journal of Biochemistry

      Volume: 171 Issue: 4 Pages: 429-441

    • DOI

      10.1093/jb/mvab154

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of a giant photosystem I supercomplex in the symbiotic dinoflagellate Symbiodiniaceae2020

    • Author(s)
      Hiroki Kato, Ryutaro Tokutsu, Hisako Kubota-Kawai, Raymond N. Burton-Smith, Eunchul Kim, Jun Minagawa
    • Journal Title

      Plant Physiology

      Volume: 183 Issue: 4 Pages: 1725-1734

    • DOI

      10.1104/pp.20.00726

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] Structure and function of photosystem I complex2021

    • Author(s)
      Hisako Kubota-Kawai
    • Organizer
      First International Conference on Technologies for Smart Green Connected Society 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Investigation on the thermodynamic dissociation kinetics of Photosystem I trimer to determine the binding strengths of each protomer2021

    • Author(s)
      Hisako Kubota-Kawai
    • Organizer
      第59回 日本生物物理学会年会シンポジウム
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Firmicutes bacterium 由来のCDP-DAG合成酵素Tam41の結晶構造の解明2020

    • Author(s)
      木村啓介, 河合文啓, 河合寿子, 小島理恵子, 渡邊康紀, 遠藤斗志也, 田村康
    • Organizer
      第28 回 山形分子生物学セミナー
    • Related Report
      2020 Research-status Report
  • [Presentation] Crystal Structure of Tam41 CDP-DAG synthase from Firmicutes bacterium2020

    • Author(s)
      木村 啓介, 河合 文啓, 平田 邦生, 河合 寿子, 小島 理恵子, 渡邊 康紀, 遠藤 斗志也, 田村 康
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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