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Investigating the physiological function and molecular mechanisms of cis-NATs responding to TGF_beta

Research Project

Project/Area Number 20K06541
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43030:Functional biochemistry-related
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

Ohhata Tatsuya  浜松医科大学, 医学部, 助教 (80616459)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsTGFβ / cis-NATs / プログラム / EMT / Epigenetics / Program / TGF-beta / アンチセンスRNA / cisNATs
Outline of Research at the Start

TGFβは腫瘍抑制作用と腫瘍促進作用の二面性を持つ増殖因子であり、その生理作用は多彩である。一方、cis-NATs とは、センス鎖と同じ領域から転写される逆方向の転写産物である。本提案では、TGFβ応答遺伝子のcis-NATs を①体系的に探索・編纂し、その②生理的機能の解明および③分子機構の解明を目標とする。本研究成果は、cis-NATs の一般性や生理機能・分子機構のみならず、TGFβ 応答反応の新機構の理解にも大きく貢献する。

Outline of Final Research Achievements

TGFβ is a growth factor with dual functions to promote or suppress tumor growth. cis-NATs are a class of antisense transcripts transcribed from the same genomic region of the sense strand. In this proposal, we attempted to comprehensively identify the TGFβ-responsive cis-NATs and elucidate their physiological functions and molecular mechanisms.
In this study, we developed an original program called CCIVR and successfully identified several functional TGFβ-responsive cis-NATs (Ohhata T et al., Sci Rep 2022). In addition, we developed another original program, CCIVR2, which also facilitates the identification of non-overlapping antisense transcripts (Suzuki M et al., Sci Rep., 2023). CCIVR/CCIVR2 is therefore a valuable bioinformatics tool for the analysis of functional antisense transcripts in different species and numerous biological processes.

Academic Significance and Societal Importance of the Research Achievements

cis-NATsは、センス鎖と同領域から転写される逆方向の転写産物である。本研究では、RNA-seqデータから網羅的にcis-NATsを同定できる独自プログラムCCIVRを開発した(Ohhata T et al., Sci Rep., 2022)。さらに非オーバーラップ型のアンチセンスRNAも探索できるCCIVR2も開発した(Suzuki M et al., Sci Rep., 2023)。CCIVR/CCIVR2は異なる生物種や多くの生物学的プロセスの機能的アンチセンス転写産物の解析に寄与する。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (9 results)

All 2024 2023 2022 2021

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] CCIVR2 facilitates comprehensive identification of both overlapping and non-overlapping antisense transcripts within specified regions2023

    • Author(s)
      Suzuki Maya、Sakai Satoshi、Ota Kosuke、Bando Yuki、Uchida Chiharu、Niida Hiroyuki、Kitagawa Masatoshi、Ohhata Tatsuya
    • Journal Title

      Scientific Reports

      Volume: 24 Issue: 1 Pages: 17011-17011

    • DOI

      10.1038/s41598-023-42044-x

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CCIVR facilitates comprehensive identification of cis-natural antisense transcripts with their structural characteristics and expression profiles2022

    • Author(s)
      Ohhata Tatsuya、Suzuki Maya、Sakai Satoshi、Ota Kosuke、Yokota Hazuki、Uchida Chiharu、Niida Hiroyuki、Kitagawa Masatoshi
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 15525-15525

    • DOI

      10.1038/s41598-022-19782-5

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamics of transcription-mediated conversion from euchromatin to facultative heterochromatin at the Xist promoter by Tsix2021

    • Author(s)
      Ohhata Tatsuya、Yamazawa Kazuki、Miura-Kamio Asuka、Takahashi Saori、Sakai Satoshi、Tamura Yuka、Uchida Chiharu、Kitagawa Kyoko、Niida Hiroyuki、Hiratani Ichiro、Kobayashi Hisato、Kimura Hiroshi、Wutz Anton、Kitagawa Masatoshi
    • Journal Title

      Cell Reports

      Volume: 34 Issue: 13 Pages: 108912-108912

    • DOI

      10.1016/j.celrep.2021.108912

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] CCIVRはcis-NATの構造的特徴と発現プロファイルの包括的同定を可能にする2024

    • Author(s)
      大畑 樹也
    • Organizer
      第29回浜松医科学シンポジウム
    • Related Report
      2023 Annual Research Report
  • [Presentation] CCIVR2は特定領域内における重複・非重複アンチセンス転写産物の包括的な同定を可能にする2023

    • Author(s)
      大畑 樹也
    • Organizer
      第46回日本分子生物学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] CCIVRはcis-NATの構造的特徴と発現プロファイルの包括的同定を可能にする2023

    • Author(s)
      大畑 樹也
    • Organizer
      第7回北陸エピジェネティクス研究会
    • Related Report
      2023 Annual Research Report
  • [Presentation] CCIVR2は指定領域のオーバーラップ型および 非オーバーラップ型アンチセンスRNAを網羅的に同定する2023

    • Author(s)
      大畑 樹也
    • Organizer
      第4回浜松医科大学研究交流会
    • Related Report
      2023 Annual Research Report
  • [Presentation] CCIVR facilitates comprehensive identification of cis-NATs with their structural characteristics and expression profiles2023

    • Author(s)
      Tatsuya Ohhata
    • Organizer
      The 20th Kyungpook_Hamamatsu National University Joint Symposium
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cis-Natural antisense transcripts(cis-NATs)を網羅的に同定する独自プログラムCCIVRの開発2022

    • Author(s)
      大畑樹也、鈴木麻耶、酒井聡、太田幸佑、北川雅敏
    • Organizer
      第45回分子生物学会
    • Related Report
      2022 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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