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Spindle function and formation regulated by opt-controllable kinesins

Research Project

Project/Area Number 20K06635
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44010:Cell biology-related
Research InstitutionThe University of Tokyo

Principal Investigator

Yajima Junichiro  東京大学, 大学院総合文化研究科, 教授 (00453499)

Co-Investigator(Kenkyū-buntansha) 須河 光弘  東京大学, 大学院総合文化研究科, 助教 (80626383)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsモータータンパク質 / 分裂期キネシン / 光応答性タンパク質 / 紡錘体 / キネシン
Outline of Research at the Start

細胞分裂において、紡錘体の構成分子である分裂期キネシンなどが、適切に機能し紡錘体が形成される。本研究では、可視光照射のON/OFFにより分裂期キネシンの機能のON/OFF制御を可能とする「メカノ-オプトジェネティクスアプローチ」を開発し、力学的機能を果たすキネシンを、分裂過程の任意のタイミングで局所的に不活性化させ、目的のキネシンの機能を定量し、動的な構造体である紡錘体を巧みに統御する生命独特の分裂システムの分子機構の一端を明らかにすることを目的とする。

Outline of Final Research Achievements

For kinesin-6 functioning during anaphase, it was found, in an in vitro experiment, that single kinesin-6 molecules in dimeric forms were able to move processively on the microtubules under conditions of very low external load. We also found that kinesin-6 molecules move helically along suspended microtubules. On the other hand, in the presence of external load, their processivity were low. With a light-responsive protein for human kinesin-5 functioning during metaphase, we investigated the conditions regulating the binding and dissociation of kinesin-5 to microtubules with and without light irradiation at specific wavelengths.

Academic Significance and Societal Importance of the Research Achievements

モータータンパク質キネシンは多種類存在し、各種キネシンは微小管上で力を発生し、細胞分裂等の物理的な現象に関わる酵素である。細胞分裂の分子機構を理解するには各種キネシンの力学的特性を明らかにしてゆくことも重要であり、本研究ではキネシン-6の力学特性の一端を明らかにした点で学術的意義がある。また、キネシンの力発生を従来法よりも迅速かつ局所的に制御可能な可視光照射で行う手法の開発を進めた。今後、キネシン機能の光制御法の確立によって細胞分裂中の各種キネシン機能が明らかになることが期待できる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (9 results)

All 2022 2021 2020

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (3 results) (of which Invited: 1 results)

  • [Journal Article] Motor generated torque drives coupled yawing and orbital rotations of kinesin coated gold nanorods2022

    • Author(s)
      Sugawa Mitsuhiro、Maruyama Yohei、Yamagishi Masahiko、Cross Robert A.、Yajima Junichiro
    • Journal Title

      Communications Biology

      Volume: 5 Issue: 1 Pages: 1368-1368

    • DOI

      10.1038/s42003-022-04304-w

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Anchoring geometry is a significant factor in determining the direction of kinesin-14 motility on microtubules2022

    • Author(s)
      Yamagishi Masahiko、Sumiyoshi Rieko、Drummond Douglas R.、Yajima Junichiro
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 15417-15417

    • DOI

      10.1038/s41598-022-19589-4

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Torque generating properties of Tetrahymena ciliary three-headed outer-arm dynein2022

    • Author(s)
      Yamaguchi Shin、Yamagishi Masahiko、Yajima Junichiro
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 16722-16722

    • DOI

      10.1038/s41598-022-21001-0

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of the motility of monomeric kinesin-5/Cin82021

    • Author(s)
      Yamagishi Masahiko、Maruyama Yohei、Sugawa Mitsuhiro、Yajima Junichiro
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 555 Pages: 115-120

    • DOI

      10.1016/j.bbrc.2021.03.134

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three-dimensional tracking of the ciliate Tetrahymena reveals the mechanism of ciliary stroke-driven helical swimming2021

    • Author(s)
      Marumo Akisato、Yamagishi Masahiko、Yajima Junichiro
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1

    • DOI

      10.1038/s42003-021-02756-0

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-62021

    • Author(s)
      Maruyama Yohei、Sugawa Mitsuhiro、Yamaguchi Shin、Davies Tim、Osaki Toshihisa、Kobayashi Takuya、Yamagishi Masahiko、Takeuchi Shoji、Mishima Masanori、Yajima Junichiro
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1

    • DOI

      10.1038/s42003-021-01704-2

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] The movement of kinesin with the neck linker hanging free in solution2022

    • Author(s)
      Rieko Sumiyoshi, Masahiko Yamagishi, Mitsuhiro Sugawa, Junichiro Yajima
    • Organizer
      第60回生物物理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] A chiral torque component present in bio-nanomachine kinesins.2020

    • Author(s)
      Junichiro Yajima
    • Organizer
      第46回分子生物学会年会シンポジウム
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 繊毛虫Tetrahymenaによる遊泳軌跡の三次元定量2020

    • Author(s)
      丸茂 哲聖, 松田 恭平, 山岸 雅彦, 須河 光弘, 矢島 潤一郎
    • Organizer
      第58回生物物理学会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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