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evolutionarily conserved mechanisms underlying cellular adaptation to glucose-limited conditions

Research Project

Project/Area Number 20K06648
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44010:Cell biology-related
Research InstitutionKurume University

Principal Investigator

Saitoh Shigeaki  久留米大学, 付置研究所, 教授 (30352123)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords糖代謝 / グルコース / TORC2 / ミトコンドリア / 分裂酵母 / ヒト培養細胞 / 栄養飢餓ストレス / グルコース飢餓 / 遺伝子スクリーニング / ヒト細胞 / ブドウ糖 / ヘキソース / 栄養 / 飢餓 / 細胞応答
Outline of Research at the Start

人間を含むほとんどすべての真核細胞にとって、グルコースやフルクトースなどのヘキソースは主要なエネルギー源・炭素源であるが、細胞を取り囲む微小環境中に常に潤沢なヘキソースが存在するわけではない。本研究では、「ヘキソース濃度が低い環境に、真核細胞はどのような仕組みで順応するのか?」を核心的な「問い」とする。ヒト培養細胞と分裂酵母細胞をモデル生物とした解析を実施し、「低ヘキソース環境への順応」に不可欠な遺伝子を網羅的に同定する。さらに、環境中のヘキソース濃度の低下を感知する「センサー機構」の実体を明らかにする。

Outline of Final Research Achievements

While hexose is the primary source of carbon in most eukaryotes including humans, abundant hexose is not always available in microenvironments surrounding eukaryote cells. Thus, eukaryote cells possess molecular mechanisms enabling adaptation to hexose-limited environments.
In this study, to reveal the evolutionarily conserved mechanisms underlying adaptation to hexose-limited environments, we utilized fission yeast and human cultured cells and screened for genes involved in such mechanisms. Mitochondria functions, and regulation of endocytosis via TORC2 signaling pathway are found to play pivotal roles in cellular adaptation to hexose-limited environments.

Academic Significance and Societal Importance of the Research Achievements

本研究課題で言う「低ヘキソース環境」とは、健常人の血糖値と同程度のヘキソースを含む培養環境である。それゆえ本研究は「正常血糖値レベルのヘキソースを含む体液中で、なぜヒト細胞は活動できるのか?」という問いに答える研究であると言える。本研究の成果はがんや2型糖尿病の診断や治療法の開発につながるものと期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (8 results)

All 2023 2022 2021

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 7 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast <i>S. pombe</i>2023

    • Author(s)
      Ishikawa Ken、Soejima Saeko、Saitoh Shigeaki
    • Journal Title

      Journal of Cell Science

      Volume: 136 Issue: 9

    • DOI

      10.1242/jcs.261186

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of hexose transporter genes in the views of the chronological life span and glucose uptake in fission yeast2022

    • Author(s)
      Maruyama Teppei、Hayashi Kanako、Matsui Kotaro、Maekawa Yasukichi、Shimasaki Takafumi、Ohtsuka Hokuto、Shigeaki Saitoh、Aiba Hirofumi
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 68 Issue: 6 Pages: 270-277

    • DOI

      10.2323/jgam.2022.05.006

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Growth conditions inducing G1 cell cycle arrest enhance lipid production in the oleaginous yeast <i>Lipomyces starkeyi</i>2022

    • Author(s)
      Morimoto Yasutaka、Saitoh Shigeaki、Takayama Yuko
    • Journal Title

      Journal of Cell Science

      Volume: 135 Issue: 16

    • DOI

      10.1242/jcs.259996

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TORC2 inhibition of α-arrestin Aly3 mediates cell surface persistence of S. pombe Ght5 glucose transporter in low glucose2021

    • Author(s)
      Toyoda Y, Soejima S, Masuda F, Saitoh S.
    • Journal Title

      Journal of Cell Science

      Volume: 134 Issue: 10 Pages: 1-14

    • DOI

      10.1242/jcs.257485

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fission yeast TORC2 signaling pathway ensures cell proliferation under glucose-limited, nitrogen-replete conditions2021

    • Author(s)
      Toyoda Y, Saitoh S.
    • Journal Title

      Biomolecules

      Volume: 11 Issue: 10 Pages: 1-8

    • DOI

      10.3390/biom11101465

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins2021

    • Author(s)
      Uehara Lisa、Saitoh Shigeaki、Mori Ayaka、Sajiki Kenichi、Toyoda Yusuke、Masuda Fumie、Soejima Saeko、Tahara Yuria、Yanagida Mitsuhiro
    • Journal Title

      Open Biology

      Volume: 11 Issue: 4 Pages: 200369-200369

    • DOI

      10.1098/rsob.200369

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Implementation of dCas9-mediated CRISPRi in the fission yeastSchizosaccharomyces pombe2021

    • Author(s)
      Ishikawa Ken、Soejima Saeko、Masuda Fumie、Saitoh Shigeaki
    • Journal Title

      G3 Genes|Genomes|Genetics

      Volume: 11 Issue: 4

    • DOI

      10.1093/g3journal/jkab051

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Patent(Industrial Property Rights)] 情報取得装置、プログラム、組換えベクターの製造方法及び遺伝子発現抑制方法2021

    • Inventor(s)
      石川健、齋藤成昭
    • Industrial Property Rights Holder
      石川健、齋藤成昭
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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