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Analysis of the mechanisms that determine and maintain the tail ratio

Research Project

Project/Area Number 20K06656
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44020:Developmental biology-related
Research InstitutionTottori University

Principal Investigator

TAKEUCHI Takashi  鳥取大学, 医学部, 教授 (70197268)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords組織比率 / 尾 / イベリアトゲイモリ / ゲノム編集 / Hox13 / Hox / 器官サイズ比 / 尾長 / 椎骨 / hox / 椎骨数 / 軟骨形成 / 椎骨サイズ / 器官サイズ / 形態形成
Outline of Research at the Start

動物の器官や四肢、尾などのサイズはそれぞれの種固有の比率をもつ。また、再生できる動物では再生過程でもこの比率が再獲得される。しかし、どのようなしくみでこの比率が決まり、維持され、そして再獲得されるかについてはほとんどわかっていない。私たちは個体全体の形成に深く関わるhox遺伝子の一群を破壊したところ、著しい尾の伸長(野生型の2倍以上)を見出した。この結果は、これらの遺伝子が尾の伸長を抑制することでイモリ固有の尾の比率の決定や維持に強く関与することを示している。本研究では、この変異体の解析や移植実験を基盤に発生および再生過程での尾の比率の決定と維持を指令するカスケードの解明をめざす。

Outline of Final Research Achievements

Hox13 mutant newts produced by genome editing show a marked increase in tail length. To understand the mechanisms that determine and maintain tissue size ratios, which largely remain unrknown, we focused on this mutant and obtained the following results. (1) We confirmed that the abnormal tail length is caused by mutation of the Hox13 genes themselves, especially a13 and c13, by generating germline mutants. (2) The abnormal tail length is caused by an abnormal increase in the number of caudal vertebrae. (3) All paralogous genes of Hox13 and marker genes of mesodermal progenitors and neural progenitors are expressed at the tail tips of wild-type adults. These results suggest that Hox13 regulates the differentiation and proliferation of progenitor cells in the tail tips and regulates the tail length ratio to be constant.

Academic Significance and Societal Importance of the Research Achievements

ヒトも含めてほとんどの動物の組織の比率はほぼ一定になるように制御されている。その破綻は健常な生命活動を困難にする。また、この制御は様々な動物が環境に適応しながら生存するために必須である。しかし、その仕組みはほとんど解明されていない。遺伝学的にこの課題を困難にしてきた理由の一つは組織比率が異常になる変異体がほとんど発見されていないことにある。本研究はまさにその変異体に注目し、その制御機構の一端を解明した。本研究の発展により様々な組織の比率が一定になる制御機構の一般原理の解明が期待できる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (38 results)

All 2023 2022 2021 2020 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (22 results) (of which Int'l Joint Research: 8 results,  Invited: 3 results) Remarks (11 results)

  • [Journal Article] Appendage‐restricted gene induction using a heated agarose gel for studying regeneration in metamorphosed Xenopus laevis and Pleurodeles waltl2023

    • Author(s)
      Matsubara Haruka、Kawasumi‐Kita Aiko、Nara Saki、Yokoyama Hibiki、Hayashi Toshinori、Takeuchi Takashi、Yokoyama Hitoshi
    • Journal Title

      Development, Growth & Differentiation

      Volume: 65 Issue: 2 Pages: 86-93

    • DOI

      10.1111/dgd.12841

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Newt Hoxa13 has an essential and predominant role in digit formation during development and regeneration2022

    • Author(s)
      Takeuchi Takashi、Matsubara Haruka、Minamitani Fumina、Satoh Yukio、Tozawa Sayo、Moriyama Tomoki、Maruyama Kohei、Suzuki Ken-ichi T.、Shigenobu Shuji、Inoue Takeshi、Tamura Koji、Agata Kiyokazu、Hayashi Toshinori
    • Journal Title

      Development

      Volume: 149 Issue: 5

    • DOI

      10.1242/dev.200282

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photoperiod-independent testicular development in the model newt Pleurodeles waltl2021

    • Author(s)
      Kyakuno, M. Nakamori, R. Tazawa, I. Uemasu, H. Namba, N. Tsunekawa, N. Noce, T. Satoh, Y. Takeuchi, T. Hayashi, T.
    • Journal Title

      Dev Growth Differ

      Volume: 63 Issue: 6 Pages: 277-284

    • DOI

      10.1111/dgd.12738

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cryo-injury procedure-induced cardiac regeneration shows unique gene expression profiles in the newt Pleurodeles waltl.2021

    • Author(s)
      Uemasu H, Ikuta H, Igawa T, Suzuki M, Kyakuno M, Iwata Y, Tazawa I, Ogino H, Satoh Y, Takeuchi T, Namba N, Hayashi T.
    • Journal Title

      Dev Dyn

      Volume: - Issue: 5 Pages: 864-876

    • DOI

      10.1002/dvdy.450

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A non-mosaic transchromosomic mouse model of Down syndrome carrying the long arm of human chromosome 212020

    • Author(s)
      Kazuki Yasuhiro、Gao Feng J、Li Yicong、Moyer Anna J、Devenney Benjamin、Hiramatsu Kei、Miyagawa-Tomita Sachiko、Abe Satoshi、Kazuki Kanako、Kajitani Naoyo、Uno Narumi、Takehara Shoko、Takiguchi Masato、Yamakawa Miho、Hasegawa Atsushi、Shimizu Ritsuko、Matsukura Satoko、Noda Naohiro、et al.
    • Journal Title

      eLife

      Volume: 9 Pages: 1-29

    • DOI

      10.7554/elife.56223

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Multiple disruption of newt Hox13 paralogs revealed that Hoxa13 has an essential and predominant role in digit formation during limb development and regeneration2022

    • Author(s)
      Takeuchi, T., Matsubara, H., Satoh, Y., Tozawa, S., and Hayashi, T.
    • Organizer
      Salamander Meeting 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Newt Hoxa13 has an essential and predominant role in digit formation during development and regeneration2022

    • Author(s)
      Takashi Takeuchi, Haruka Matsubara, Fumina Minamitani, Yukio Satoh, Sayo Tozawa, Tomoki Moriyama, Kohei Maruyama, Ken-ichi T. Suzuki, Shuji Shigenobu, Takeshi Inoue, Koji Tamura, Kiyokazu Agata, Toshinori Hayashi
    • Organizer
      第55回発生生物学会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Tbx3 differently regulate digits morphogenesis of the fore- and hindlimbs in the newt (Pleurodeles waltl)2022

    • Author(s)
      Haruka Matsubara, Takashi Takeuchi
    • Organizer
      第55回発生生物学会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The mechanism of hindlimb defect in Hox13 mutant newts2022

    • Author(s)
      Sayo Tozawa, Haruka Matsubara, Fumina Minamitani, Yukio Satoh, Toshinori Hayashi, Takashi Takeuchi
    • Organizer
      第55回発生生物学会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation of the initiation mechanism of testicular regeneration in the newt2022

    • Author(s)
      Mai Takehara, Mitsuki Kyakuno, Yukio Sato, Takashi Takeuchi, Ichiro Tazawa, Nobuaki Furuno, Toshinori Hayashi
    • Organizer
      第55回発生生物学会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of the progression of cell proliferation in CDK1 knockout newts2022

    • Author(s)
      Yuta Nakao, Ichiro Tazawa, Nobuaki Furuno, Takashi Takeuchi , Toshinori Hayashi
    • Organizer
      第55回発生生物学会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Function of cyclin D1 in the regulation of the cell cycle in newts2022

    • Author(s)
      Hiromi Ikuta, Hitoshi Uemasu, Shouhei Azuma, Takeshi Igawa, Ichiro Tazawa1,2, Nobuaki Huru no, Noriyuki Namba, Hajime Ogino, Takashi Takeuchi, Toshinori Hayashi
    • Organizer
      第55回発生生物学会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イモリ指形態とShhシグナルの関係性2022

    • Author(s)
      松原遼、竹内隆
    • Organizer
      第93回動物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] イモリHox9およびHox10の四肢形成における機能2022

    • Author(s)
      裏川浩平、松原遼、阿部玄武、竹内隆
    • Organizer
      第93回動物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] イベリアトゲイモリの発生や再生におけるHox遺伝子の機能解析2022

    • Author(s)
      竹内隆、松原遼、戸澤紗代、伊藤優、裏川浩平、明日香智絵、林利憲
    • Organizer
      第3回イベリアトゲイモリ研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] イベリアトゲイモリの四肢形成におけるHox9/10遺伝子の機能解析2022

    • Author(s)
      裏川浩平、松原遼、阿部玄武、竹内隆
    • Organizer
      第3回イベリアトゲイモリ研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] イベリアトゲイモリの四肢形成におけるHox11-13の機能について2022

    • Author(s)
      伊藤優、松原遼、戸澤紗代、裏川浩平、明日香智絵、林利憲、竹内隆
    • Organizer
      第3回イベリアトゲイモリ研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Hox13変異イモリにおける後肢欠損の仕組みについて2022

    • Author(s)
      戸澤紗代、松原遼、南谷史奈、佐藤幸夫、林利憲、竹内隆
    • Organizer
      第3回イベリアトゲイモリ研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 四肢の切断面より基部領域で除去した骨格が再生する2022

    • Author(s)
      阿南有紗、松原遼、阿部玄武、竹内隆
    • Organizer
      第3回イベリアトゲイモリ研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Function of cyclin D1 in the regulation of the cell cycle in newts2022

    • Author(s)
      Hiromi Ikuta, Hitoshi Uemasu, Shouhei Azuma, Takeshi Igawa, Ichiro Tazawa, Nobuaki Huru no, Noriyuki Namba, Hajime Ogino, Takashi Takeuchi, Toshinori Hayashi
    • Organizer
      第3回イベリアトゲイモリ研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] CDK1 ノックアウトイモリにおける細胞増殖の進行の解析2022

    • Author(s)
      Yuta Nakao, Ichiro Tazawa, Nobuaki Furuno, Takashi Takeuchi , Toshinori Hayashi
    • Organizer
      第3回イベリアトゲイモリ研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] イモリの精巣再生を開始させる機構の研究2022

    • Author(s)
      Mai Takehara, Mitsuki Kyakuno, Yukio Sato, Takashi Takeuchi, Ichiro Tazawa, Nobuaki Furuno, Toshinori Hayashi
    • Organizer
      第3回イベリアトゲイモリ研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Changes in the pattern of genes expression could provide the fore- and hindlimb digital pattern of the newt (Pleurodeles waltl)2021

    • Author(s)
      Haruka Matsubaraand Takashi Takeuchi
    • Organizer
      日本発生生物学会年会
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] イモリにおける精巣再生機構の解明2021

    • Author(s)
      竹原 舞、客野 瑞月、佐藤 幸雄、竹内 隆、田澤 一朗、古野 伸明、林 利憲
    • Organizer
      日本動物学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] イモリ後肢発生におけるHox13遺伝子の機能解析2021

    • Author(s)
      戸澤 紗代、南谷 史菜、林 利憲、松原 遼、竹内 隆
    • Organizer
      日本動物学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] イモリ四肢発生における前後軸形成遺伝子の指形態形成への機能2021

    • Author(s)
      松原 遼、竹内 隆
    • Organizer
      日本動物学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] イベリアトゲイモリの四肢発生・再生におけるHox13遺伝子の機能解析2021

    • Author(s)
      竹内 隆、松原 遼、南谷 史菜、佐藤 幸夫、戸澤 紗代、林 利憲
    • Organizer
      第3回 再生学異分野融合研究会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Remarks] 鳥取大学医学部生命科学科発生生物学分野

    • URL

      https://www.med.tottori-u.ac.jp/introduction/lifesciences/about/3319/3335/23806.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] 鳥取大学医学部生命科学科 研究室紹介 発生生物学分野

    • URL

      https://www.med.tottori-u.ac.jp/lifesciences/research/staff_takeuchi.html

    • Related Report
      2022 Annual Research Report
  • [Remarks]

    • URL

      https://www.facebook.com/seitai.seimei.toridai?ref=aymt_homepage_panel

    • Related Report
      2021 Research-status Report
  • [Remarks]

    • URL

      https://www.med.tottori-u.ac.jp/introduction/lifesciences/about/3319/3335/23806.html

    • Related Report
      2021 Research-status Report
  • [Remarks]

    • URL

      https://www.med.tottori-u.ac.jp/lifesciences/research/staff_takeuchi.html

    • Related Report
      2021 Research-status Report
  • [Remarks]

    • URL

      https://www.med.tottori-u.ac.jp/lifesciences/admissions/opencampus/opencampus_movie_long.html

    • Related Report
      2021 Research-status Report
  • [Remarks]

    • URL

      https://www.med.tottori-u.ac.jp/lifesciences/topics/essay/31713.html

    • Related Report
      2021 Research-status Report
  • [Remarks]

    • URL

      https://www.facebook.com/seitai.seimei.toridai?ref=aymt_homepage_panel

    • Related Report
      2020 Research-status Report
  • [Remarks]

    • URL

      https://www.med.tottori-u.ac.jp/introduction/lifesciences/about/3319/3335/23806.html

    • Related Report
      2020 Research-status Report
  • [Remarks]

    • URL

      https://www.med.tottori-u.ac.jp/lifesciences/research/staff_takeuchi.html

    • Related Report
      2020 Research-status Report
  • [Remarks]

    • URL

      https://www.med.tottori-u.ac.jp/lifesciences/admissions/opencampus/opencampus_movie_long.html

    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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