• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Activation mechanisms of B2/B3 Raf kinases in plant stress responses

Research Project

Project/Area Number 20K06680
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionSaitama University

Principal Investigator

Takezawa Daisuke  埼玉大学, 理工学研究科, 教授 (20281834)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords植物 / ストレス / 環境 / プロテインキナーゼ / アブシジン酸 / 乾燥 / プロテインホスファターゼ / アブシシン酸 / オスモストレス / RAFキナーゼ / 受容体 / キナーゼ / コケ植物
Outline of Research at the Start

植物は、異常気象などによる水不足に対し、これに反応して枯死を防ぐための分子機構をもつと考えられている。しかし水不足を感知する仕組みや、その情報をどのように細胞内に伝達するかといった仕組みには不明な点が多い。最近、「ARK」と呼ばれるタンパク質リン酸化酵素が植物の水不足反応の伝達プロセスに関わっていることが明らかとなり、我々はARKの活性化のメカニズムを調べることで、植物の水不足反応に関わる制御機構を解明できるのではないかと考えた。その仕組みは、植物に共通の環境感知の仕組みとも密接に関わっているはずであり、本研究により異常気象下でも植物を生育させるための基礎的知見が得られると期待している。

Outline of Final Research Achievements

Phytohormone abscisic acid (ABA) plays a key role in stomata closure, osmostress acclimation, and vegetative and embryonic dormancy in plants. Group B2/B3 Raf protein kinases (B2/B3-Rafs) serve as positive regulators of ABA and osmostress signaling in both mosses and liverworts as well as in the angiosperm Arabidopsis thaliana. While P. patens has a single B3-Raf called ARK, specific members of B3-Rafs among six paralogs regulate ABA and osmostress signaling in A. thaliana, indicating functional diversification of B3-Rafs. It was also found that the liverwort Marchantia polymorpha, belonging to liverworts, has three paralogs of B3-Rafs, MpARK1, MpARK2 and MpARK3, and MpARK4 and MpARK5 with structural variations in the non-kinase region toward the N terminus of the polypeptide. These studies have shed light on the role of the regulatory domains located in the N-terminal domain on the regulation of these Raf kinases.

Academic Significance and Societal Importance of the Research Achievements

高いレベルの環境ストレス耐性を持つコケ植物は、高い相同組み換え率やゲノム編集効率など、材料としても多くの利点がある。本研究で得られた成果は、被子植物にも保存される環境ストレス耐性の仕組みについても明らかにできると期待され、実際にコケ植物で明らかにされたB3-RAFの重要性は被子植物でも同様であることがのちに示された。こうしたアプローチは、シロイヌナズナなどのモデル系を用いた研究を補完するだけでなく、有用作物で未同定のストレス耐性関連因子の同定に寄与し、社会にも貢献しうる研究である。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (14 results)

All 2022 2021 2020

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (9 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Targeted in vivo mutagenesis of a sensor histidine kinase playing an essential role in ABA signaling of the moss Physcomitrium patens2022

    • Author(s)
      Sk R, Miyabe MT, Takezawa D, Yajima S, Yotsui I, Taji T, Sakata Y
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 637 Pages: 93-99

    • DOI

      10.1016/j.bbrc.2022.11.009

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differential regulations of abscisic acid-induced desiccation tolerance and vegetative dormancy by group B3 Raf kinases in liverworts2022

    • Author(s)
      Jahan Akida、Yamazaki Yuto、Islam Mousona、Ghosh Totan Kumar、Yoshimura Nami、Kato Hirotaka、Ishizaki Kimitsune、Shinozawa Akihisa、Sakata Yoichi、Takezawa Daisuke
    • Journal Title

      Frontiers in Plant Science

      Volume: 13

    • DOI

      10.3389/fpls.2022.952820

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sensor histidine kinases mediate ABA and osmostress signaling in the moss Physcomitrium patens2022

    • Author(s)
      Tsukasa Toriyama, Akihisa Shinozawa, Yuki Yasumura, Masashi Saruhashi, Mayuka Hiraide, Shiori Ito, Hideyuki Matsuura, Keiko Kuwata, Mika Yoshida, Tadashi Baba, Izumi Yotsui, Teruaki Taji, Daisuke Takezawa, Yoichi Sakata
    • Journal Title

      Current Biology

      Volume: 32 Issue: 1 Pages: 164-175

    • DOI

      10.1016/j.cub.2021.10.068

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK22021

    • Author(s)
      Kamiyama Yoshiaki、Hirotani Misaki、Ishikawa Shinnosuke、Minegishi Fuko、Katagiri Sotaro、Rogan Conner J.、Takahashi Fuminori、Nomoto Mika、Ishikawa Kazuya、Kodama Yutaka、Tada Yasuomi、Takezawa Daisuke、Anderson Jeffrey C.、Peck Scott C.、Shinozaki Kazuo、Umezawa Taishi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 118 Issue: 30

    • DOI

      10.1073/pnas.2100073118

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Activation of SnRK2 by Raf-like kinase ARK represents a primary mechanism of ABA and abiotic stress responses2020

    • Author(s)
      Islam Mousona、Inoue Takumi、Hiraide Mayuka、Khatun Nobiza、Jahan Akida、Kuwata Keiko、Katagiri Sotaro、Umezawa Taishi、Yotsui Izumi、Sakata Yoichi、Takezawa Daisuke
    • Journal Title

      Plant Physiology

      Volume: 185 Issue: 2 Pages: 533-546

    • DOI

      10.1093/plphys/kiaa046

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 植物低温応答におけるRaf様プロテインキナーゼARKの役割2022

    • Author(s)
      石田哲也、竹澤大輔、井上拓己
    • Organizer
      第67回低温生物工学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 重イオンビーム照射によるゼニゴケアブシシン酸低感受性変異株の単離と解析2022

    • Author(s)
      平野 真彩、Mousona Islam、Jahan Akida、長谷川 綾香、山崎 優斗、篠澤 章久、坂田 洋一、竹澤 大輔
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ヒメツリガネゴケの低温馴化におけるRaf様プロテインキナーゼARKの量的変動と耐凍性2022

    • Author(s)
      石田哲也、井上拓巳、坂田洋一、竹澤大輔
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] The role of protein phosphatase 2C in abiotic stress in the moss Physcomitrium patens2021

    • Author(s)
      Ikegami K, Takezawa D
    • Organizer
      12th International Plant Cold Hardiness Seminar
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional analysis RAF-like kinases in streptophytes2021

    • Author(s)
      Yamazaki Y, Jahan A, Islam M, Shinozawa A, Sakata, Y, Takezawa D
    • Organizer
      12th International Plant Cold Hardiness Seminar
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Role of ETHYLENE INSENSITIVE3 orthologs in abiotic stress responses in the moss Physcomitrium patens2021

    • Author(s)
      Islam M, Takezawa D
    • Organizer
      12th International Plant Cold Hardiness Seminar
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] The role of plant Raf-like kinase ARK in cold acclimation of the moss Physcomitrium patens2021

    • Author(s)
      Ishida T, Inoue T, Takezawa D
    • Organizer
      12th International Plant Cold Hardiness Seminar
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Group A プロテインフォスファターゼ に依存しないABA応答制御機構の解明2021

    • Author(s)
      池上 慶太、平出 真由佳、坂田 洋一、竹澤 大輔
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ストレプト植物RAF様キナーゼの機能解析2021

    • Author(s)
      山崎 優斗、Akida Jahan、Mousona Islam、坂山 英俊、西山 智明、堀 孝一、太田 啓之、皆川 純、篠澤 章久、坂田 洋一、竹澤 大輔
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Research-status Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi