| Project/Area Number |
20K06699
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44030:Plant molecular biology and physiology-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | vernalization / life history / flowering locus C / perennial / cold sensing / chromatin |
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| Outline of Research at the Start |
Many plants flower after winter (vernalization), including life forms with single (annuals) and multiple (perennials) flowering. In nature, vernalization provides an adaptation to local climates and in agriculture, it influences production of many crucifer crops (cabbage, daikon, wasabi and more). The central gene of vernalization and cold sensing components were studied in annuals but not in perennials. Understanding genetic control of perennial vernalization will allow introduction of perennial features into future crops, which, like all perennials, should survive unprotected in nature.
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| Outline of Final Research Achievements |
Flowering Locus C is a central repressor of flowering in the crucifer family. While exceptionally well characterised in winter annual growth habits, its function had not been studied genetically in crucifers with perennial growth habit, which are less amenable genetic systems but they represent the ancestral forms. In this work, we created mutants in the Flowering Locus C ortholgue in Arabidopsis halleri gemmifera, a perennial crucifer from Japan with a unique growth habit. After optimizing several steps of the tissue culture Agrobacterium-mediated transformation in Ahg, we were able to obtain a limited number of primary transgenics expressing siRNA against AhgFLC. Close investigation of the mutant phenotype pointed to several perennial traits that AhgFLC controls, largely in line with expectations. These phenotypes remain to be substantiated in sequent generations. This work contributes to general understanding of the genetic determinants of annual vs perennial growth habits.
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| Academic Significance and Societal Importance of the Research Achievements |
Vernalization contributed to adaptation to diverse climates and diversification of life histories and was utilised in breeding. Together with work from Arabidopsis thaliana, this work contributes to general understanding of the genetic determinants of annual vs perennial growth habits.
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