• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular Mechanism and Physiological Function of a New Type of Aquaporin Exhibiting Dual Transporting

Research Project

Project/Area Number 20K06708
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionOkayama University

Principal Investigator

Katsuhara Maki  岡山大学, 資源植物科学研究所, 教授 (00211847)

Co-Investigator(Kenkyū-buntansha) 堀江 智明  信州大学, 学術研究院繊維学系, 教授 (90591181)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2023: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsアクアポリン / 水輸送 / イオン輸送 / イネ / オオムギ / 塩ストレス / 生体膜 / イオンチャネルアクアポリン / icAQP / 電気生理 / PIPアクアポリン / OsPIP / アフリカツメガエル卵母細胞 / ナトリウム / カリウム / ナトリウム透過
Outline of Research at the Start

水輸送機能と同時にナトリウム輸送機能も合わせ持つ、すなわち2つの異なる輸送機能をデュアルで示す植物の新型アクアポリンに注目した研究である。
水とナトリウムの輸送が重要となる塩ストレス環境において、この輸送体がどのような役割を果たしているかをイネとオオムギ明らかにする。ナトリウムを含むイオン輸送の分子レベルでの性質は、アフリカツメガエル卵母細胞を使った異種機能発現解析系に電気生理測定を組み合わせた実験系で解析する。植物体においては塩ストレス応答における該当アクアポリンの生理機能を明らかにする。この研究によって、新型アクアポリンの輸送機能の分子的な機構と、耐塩性機構における役割とを明らかにする。

Outline of Final Research Achievements

We have identified new ion channel aquaporins (icAQPs), which have both water and ion transport functions in barley and rice. (1) We identified a mutated icAQP that causes loss of only water permeability but retains ion permeability and another mutated icAQP that causes loss of ion transport capacity but no change in water transport capacity. These data demonstrate that different water and ion transport pathways in the molecule. (2) We have revealed molecular mechanisms separately for water and ion permeation. (3) We have clarified that icAPQ functions in water permeability, potassium accumulation in rice roots, and sodium influx under salt stress by analyzing icAQP-deficient rice lines.

Academic Significance and Societal Importance of the Research Achievements

新型アクアポリン研究は、塩ストレス環境における水輸送とイオン輸送の交点としてのアクアポリンの未解明な役割と機能を明らかにする。これまで別々の分子が担っていると考えられてきた水とイオンの輸送を同一分子で実現している新しい分子機構が見つかり解明された。
最終的には新型アクアポリンを利用して、作物の水輸送とイオン輸送とを同時に改良する新しい栽培技術や育種につながることが期待される。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (15 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (7 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] Australian National University/The University of Adelaide(オーストラリア)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] Australian National University(オーストラリア)

    • Related Report
      2020 Research-status Report
  • [Journal Article] <i>In planta</i> evidence that the HAK transporter OsHAK2 is involved in Na+ transport in rice2023

    • Author(s)
      Morita Shigeto、Tamba Natsumi、Shibasaka Mineo、Sasano Shizuka、Kadoike Taiju、Urase Yasuyo、Maruyama Masamitsu、Fukuoka Aguri、Yanai Junta、Masumura Takehiro、Ogihara Yasunari、Satoh Shigeru、Tanaka Kunisuke、Katsuhara Maki、Nakayama Hideki
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 87 Issue: 5 Pages: 482-490

    • DOI

      10.1093/bbb/zbad020

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct Functions of the Atypical Terminal Hydrophilic Domain of the HKT Transporter in the Liverwort Marchantia polymorpha.2022

    • Author(s)
      Imran S, Oyama M, Horie R, Kobayashi NI, Costa A, Kumano R, Hirata C, Tran STH, Katsuhara M, Tanoi K, Kohchi K, Ishizaki K, Horie T.
    • Journal Title

      Plant Cell Physiology

      Volume: 63 Issue: 6 Pages: 802816-802816

    • DOI

      10.1093/pcp/pcac044

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 植物のアクアポリン2021

    • Author(s)
      且原真木
    • Journal Title

      脳神経内科

      Volume: 94 Pages: 635-641

    • Related Report
      2021 Research-status Report
  • [Journal Article] Identification and characterization of rice OsHKT1;3 Variants.2021

    • Author(s)
      Imran S, Tsuchiya Y, Tran, S.T.H., Katsuhara M
    • Journal Title

      Plants

      Volume: 10 Issue: 10 Pages: 2003-2003

    • DOI

      10.3390/plants10102006

    • NAID

      120007170519

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Survey of Barley PIP Aquaporin Ionic Conductance Reveals Ca2+-Sensitive HvPIP2;8 Na+ and K+ Conductance.2020

    • Author(s)
      Tran, S.T.H., Horie, T., Imran, S., Qiu, J., McGaughey, S., Byrt, C.S., Tyerman, S.D., Katsuhara, M.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 19 Pages: 7135-7135

    • DOI

      10.3390/ijms21197135

    • NAID

      120006937508

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 耐塩性オオムギの根水透過性 (Lpr) 下方制御に関わるアクアポリンHvPIP2;1のPhos-tag SDS-PAGEを使ったリン酸化解析2023

    • Author(s)
      大西亜耶、且原 真木
    • Organizer
      第17回トランスポーター研究会(名古屋)
    • Related Report
      2023 Annual Research Report
  • [Presentation] 植物のイオン輸送性アクアポリン2023

    • Author(s)
      且原真木、Sen Tran、小野修太朗、堀江智明
    • Organizer
      第17回トランスポーター研究会(名古屋)
    • Related Report
      2023 Annual Research Report
  • [Presentation] Na ion and K ion transport mechanism of ion channel aquaporins.2023

    • Author(s)
      Ono, S., Katsuhara, M.
    • Organizer
      Taiwan-Japan Plant Biology 2023, Taipei, Taiwan
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification, characterization, and molecular mechanisms of ion-channel aquaporins in plants.2023

    • Author(s)
      Tran, T.H.S., Ono, S., Tomoaki, H., Katsuhara, M.
    • Organizer
      Taiwan-Japan Plant Biology 2023, Taipei, Taiwan
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Plant molecular physiology of salt stress and salt tolerance mechanisms.2023

    • Author(s)
      Katsuhara, M
    • Organizer
      The 6th IBOC International Biology Conference 2023. Surabaya, Indonesia
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] イネにおけるイオン輸送性アクアポリンの同定と機能解析2022

    • Author(s)
      且原真木、Sen Tran、堀江智明
    • Organizer
      日本植物学会第86回大会(京都)
    • Related Report
      2022 Research-status Report
  • [Presentation] Ca2+-sensitive and non-selective Na+/K+ channel activity of aquaporins HvPIP2;8 and OsPIP2;4.2021

    • Author(s)
      Tran S.
    • Organizer
      International Plant WEB Forum 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Book] 根っこのふしぎな世界 1根っこってなんだろう? 第1巻 (且原真木、松波麻耶 根っこはどうやって水を吸収するの? 10-11ページ)2023

    • Author(s)
      中野明正、小泉光久
    • Total Pages
      48
    • Publisher
      文研出版
    • ISBN
      9784580825970
    • Related Report
      2023 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi