Regulatory mechanism of osteoclasts by hormones produced in osteoblasts: analysis using fish scales as a bone model
Project/Area Number |
20K06718
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 44040:Morphology and anatomical structure-related
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Research Institution | Kanazawa University |
Principal Investigator |
Suzuki Nobuo 金沢大学, 環日本海域環境研究センター, 教授 (60242476)
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Co-Investigator(Kenkyū-buntansha) |
小林 功 金沢大学, 生命理工学系, 准教授 (30774757)
関口 俊男 金沢大学, 環日本海域環境研究センター, 准教授 (40378568)
服部 淳彦 東京医科歯科大学, 教養部, 教授 (70183910)
平山 順 公立小松大学, 保健医療学部, 教授 (90510363)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Project Status |
Completed (Fiscal Year 2022)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 魚類のウロコ / ラットの頭蓋骨 / 骨芽細胞 / 破骨細胞 / メラトニン / カルシトニン / ウロコ / カルシトニンの機能阻害 / カルシトニン遺伝子の機能阻害 |
Outline of Research at the Start |
本研究では、①ウロコの再生過程におけるメラトニン合成及びカルシトニンの発現を調べて、ウロコの再生過程におけるホルモンの発現及び骨芽細胞と破骨細胞の相互作用を解析する。②カルシトニンを機能阻害をして、ウロコの再生に及ぼす影響を解析する。機能阻害の影響が、カルシトニン添加によりレスキューされるかについても調べる。③哺乳類の細胞を用いて、メラトニンによるカルシトニンの発現調節を調べる。ラットの骨芽細胞でカルシトニンの発現を免疫染色により確認しており、哺乳類で検証して、メラトニンの骨代謝制御の確証を目指す。
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Outline of Final Research Achievements |
Fish scales are bone-like tissues in which bone-formative cells (osteoblasts) and bone-resorptive cells (osteoclasts) coexist. The presence of these cells on the body surface facilitates whole-mount morphological analysis. Noting these characteristics, we have developed an assay system using scales as a bone model to investigate the effects of various hormones. In the present study, we focused on hormones expressed in scales and examined the expression of hormones (melatonin and calcitonin) produced by osteoblasts during scale regeneration. Next, hormonal treatments were applied to scales, and their effects on osteoblasts were analyzed. Mammalian bone cells were then subjected to the same hormone treatments as fish scales to examine the reproducibility of the results obtained in fish.
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Academic Significance and Societal Importance of the Research Achievements |
これまで、メラトニン及びカルシトニンは特定の内分泌腺から分泌され、分泌されたホルモンが骨に作用していると考えられていた。本研究により、魚類のウロコのみならず哺乳類の細胞(ラットの頭蓋骨及びヒトの骨芽細胞)においても、骨芽細胞で作られて、局所的に作用していることを見出すことができた。魚類のウロコを骨モデルとして用いて、哺乳類の骨芽細胞においても同様な作用を見出すことができたので、その意義は大きい。
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Possible involvement of Calcitonin I and II in calcium metabolism of the female reproductive physiology of goldfish (Carassius auratus).2023
Author(s)
Kuroda, K., Hatano, K., Kawamura, R., Fukushima, A., Sasayama, Y., Tabuchi, Y., Furusawa, Y., Ikegame, M., Hattori, A., Hirayama, J., Fukuda, T., Uekura, S., Matsubara, H., Kawago, U., Sekiguchi, T., Srivastav, A.K. and Suzuki, N.
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Journal Title
Int. Aquat. Res., 15: 15-26 (2023)
Volume: 15
Pages: 15-26
Related Report
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[Journal Article] Activation of RANKL-producing cells under simulated microgravity with a three-dimensional clinostat in regenerating goldfish scales2022
Author(s)
Yamamoto, T., Ikegame, M., Kuroda, K., Kobayashi-Sun, J., Hirayama, J., Kobayashi, I., Kawamura, R., Endo, M., Tabuchi, Y., Furusawa, Y., Yachiguchi, K., Sekiguchi, T., Matsubara, H., Yano, S., Hattori, A. and Suzuki, N.
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Journal Title
Biological Sciences in Space
Volume: 36
Issue: 0
Pages: 9-14
DOI
ISSN
0914-9201, 1349-967X
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Expression of sclerostin in the regenerating scales of goldfish and its increase under microgravity during space flight2020
Author(s)
Yamamoto, T., Ikegame, M., Hirayama, J., Kitamura, K., Tabuchi, Y., Furusawa, Y., Sekiguchi, T., Endo, M., Mishima, H., Seki, A., Yano, S., Matsubara H., Hattori, A. and Suzuki, N.
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Journal Title
Biomedical Research
Volume: 41
Issue: 6
Pages: 279-288
DOI
NAID
ISSN
0388-6107, 1880-313X
Year and Date
2020-12-01
Related Report
Peer Reviewed / Open Access
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[Journal Article] Detection of RANKL-producing cells and osteoclastic activation by the addition of exogenous RANKL in the regenerating scales of goldfish.2020
Author(s)
Yamamoto, T., Ikegame, M., Kawago, U., Tabuchi, Y., Hirayama, J., Sekiguchi, T., Furusawa, Y., Yachiguchi, K., Matsubara, H., Urata, M., Hattori, A. and Suzuki, N.
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Journal Title
Biological Science in Space
Volume: 34
Issue: 0
Pages: 34-40
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] Calcitonin I and II possible involvement of calcium metabolism in the female reproductive physiology of goldfish (Carassius auratus).2022
Author(s)
Kuroda, K., Hatano, K., Kawamura, R., Fukushima, A., Sasayama, Y., Tabuchi, Y., Furusawa, Y., Ikegame, M., Hattori, A., Hirayama, J., Matsubara, H., Kawago, U., Sekiguchi, T., Srivastav, A.K. and Suzuki, N.
Organizer
oint Usage/Joint Research on Transboundary Pollution and its Impact on Social Environment, Kanazawa University, Ishikawa, Japan
Related Report
Int'l Joint Research
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[Presentation] Glyoxal-induced formation of carboxymethyl arginine in type 1 collagen decreases both its strength and flexibility in vitro.2021
Author(s)
Hirayama, J., Kitamura, K., Tabuchi, Y., Minami, T., Matsubara, H., Hattori, A. and Suzuki, N.
Organizer
International Maillard Reaction Society-14
Related Report
Int'l Joint Research
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[Presentation] Detection of RANKL-producing cells and osteoclastic activation by the addition of RANKL in the regenerating scales of goldfish.2020
Author(s)
Yamamoto, T., Ikegame, M., Kawago, U., Tabuchi, Y., Hirayama, J., Sekiguchi, T., Furusawa, Y., Yachiguchi, K., Matsubara, H., Urata, M., Hattori, A. and Suzuki, N.
Organizer
Joint International Symposium: Challenging the Research Development and Collaboration Through Online Discussions
Related Report
Int'l Joint Research
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