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Real-time imaging of butterfly wing tissues: a system constitutive approach

Research Project

Project/Area Number 20K06724
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Otaki Joji  琉球大学, 理学部, 教授 (70360211)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords色模様形成 / 翅形成 / チョウ / リアルタイムイメージング / 多様性
Outline of Research at the Start

以下の5点について研究する。(1) 翅の外形(輪郭)決定過程を詳細に記載する。(2) 翅細胞の形態の経時変化と物理的な歪みおよび張力の作用との関係を検討し、上皮シートの歪みや張力が色模様形成を含めた翅の形態形成に果たしている役割を示す。(3) 細胞同士の横方向のコミュニケーションにサイトニーム(神経細胞の樹状突起や軸索に似た上皮細胞の長い突起)とエキソソーム(細胞外に分泌される封入体)がかかわっている可能性を明確に示す。 (4) 翅全体に走るカルシウム波を捉える。 (5) 生きた翅組織への蛍光抗体導入法を確立する。これにより、タンパク質レベルのノックダウン系を確立し、将来の機能解析につなげる。

Outline of Final Research Achievements

This study discovered that the pupal wing tissue must have a physical contact with cuticle for normal color pattern development in butterflies. Such a contact is required for morphogenic molecules from organizers to act on surrounding cells. Because chitin is the major component of cuticle, its dynamics in cells were observed. chitin objects were produced near nuclei, from which the chitin objects appear to be extended to the surface and depth of the epithelial sheet. The objects extending to the surface may be scale precursors. On the other hand, this study established a method for directly delivering proteins such as antibodies to cells of the epithelial sheet. This method enabled researchers to inhibit protein functions for color pattern formation in vivo in real-time without using a genome editing method.

Academic Significance and Societal Importance of the Research Achievements

生物の発生を考えるうえで、形成体とモルフォゲンの機能は最重要であるが、まだ解明されていない部分が多く残されている。本研究は、チョウの翅の色模様形成過程を対象にして、形成体とモルフォゲンの機能をリアルタイムイメージングを駆使して捉えようとする研究である。チョウの翅という実験系の利点を活かすことで得られた形成体とモルフォゲンに関する知見は、チョウに留まることなく、動物全体に一般化できると思われるため、本研究の学術的な意義は大きい。また、蛋白質導入方法も、さまざまな実験系に適用することができると思われ、汎用性が高い技術である。蛋白質導入法は投薬技術と直結しているため、今後さらに注目されると思われる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (5 results)

All 2023 2022 2021 2020

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results)

  • [Journal Article] Live detection of intracellular chitin in butterfly wing epithelial cells in vivo using fluorescent brightener 28: Implications for the development of scales and color patterns2023

    • Author(s)
      Yugo Nakazato, Joji M. Otaki
    • Journal Title

      Insects

      Volume: 14 Issue: 9 Pages: 753-753

    • DOI

      10.3390/insects14090753

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Protein Delivery to Insect Epithelial Cells In Vivo: Potential Application to Functional Molecular Analysis of Proteins in Butterfly Wing Development2023

    • Author(s)
      Yugo Nakazato, Joji M. Otaki
    • Journal Title

      BioTech

      Volume: 12 Issue: 2 Pages: 28-28

    • DOI

      10.3390/biotech12020028

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Butterfly Wing Color Pattern Modification Inducers May Act on Chitin in the Apical Extracellular Site: Implications in Morphogenic Signals for Color Pattern Determination2022

    • Author(s)
      Joji M. Otaki, Yugo Nakazato
    • Journal Title

      Biology

      Volume: 11 Issue: 11 Pages: 1620-1620

    • DOI

      10.3390/biology11111620

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fractal geometry of the Nymphalid Groundplan: Self-similar configuration of color pattern symmetry systems in butterfly wings2021

    • Author(s)
      Joji M. Otaki
    • Journal Title

      Insects

      Volume: 12 Issue: 1 Pages: 39-39

    • DOI

      10.3390/insects12010039

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Morphological and spatial diversity of the discal spot on the hindwings of nymphalid butterflies: Revision of the Nymphalid Groundplan2020

    • Author(s)
      Joji M. Otaki
    • Journal Title

      Insects

      Volume: 11 Issue: 10 Pages: 654-654

    • DOI

      10.3390/insects11100654

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access

URL: 

Published: 2020-04-28   Modified: 2025-01-30  

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