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Analysis of a novel organelle transference and degradation during the pollen formation process

Research Project

Project/Area Number 20K06728
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionJapan Women's University

Principal Investigator

NAGATA Noriko  日本女子大学, 理学部, 教授 (40311352)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2023: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords電子顕微鏡 / 花粉 / タペータム / 雄原細胞 / 精細胞 / オルガネラ / 葯 / タペトソーム / エライオプラスト / 液胞 / TEM / SEM / 三次元構築 / オートファジー / マイクロリポファジー / リピッドボディ / 三次元再構築
Outline of Research at the Start

本研究では、花粉形成過程において細胞間オルガネラ授受・分解という現象が実在することと、この現象が精細胞と栄養細胞それぞれの液胞によって協調的にもたらされるという仮説を検証することを目的とする。広域透過電子顕微鏡(TEM)画像取得法や走査電子顕微鏡(SEM)を用いた連続切片の三次元構築法を取り入れ、さらに蛍光-電子顕微鏡の同一切片観察法(クレム法)などを用いることで、オルガネラ授受・分解の現場を時空間的に正確に捉える。オートファジー関連の突然変異体を用いて、本現象に関わる因子を探索し、オルガネラ授受・分解のメカニズムの実体に迫る。

Outline of Final Research Achievements

We focused on organelle transference and degradation during the pollen formation process. Detailed wide-range and three-dimensional images were obtained using electron microscopy, and the following results were obtained.
1) We found accumulation of lipid bodies around generative/sperm cells and a novel microlipophagy; 2) Using sporopollenin biosynthesis-deficient mutants, we showed the details of the process of sporopollenin release from tapetum and its function; 3) We captured the process of pollen coat formation from lipidic organelles within tapetum; 4) We found that in tapetum, there is close contact between the endoplasmic reticulum membrane and the plastid envelope.

Academic Significance and Societal Importance of the Research Achievements

植物の花粉形成過程には細胞をまたいだ種々のオルガネラ授受・分解現象が存在し、これは動物には見られないユニークなものである。本研究は、植物に秘められた高度なオルガネラ制御機構の実体に迫るものである。さらに本研究は、広域及び三次元の電子顕微鏡技法を駆使することにより、細胞をまたぐような広範囲での現象を捉えることに成功した。これは異分野にも応用可能な本技術の有効性を示すものといえる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (13 results)

All 2023 2022 2021 2020

All Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 7 results) Presentation (1 results)

  • [Journal Article] Anionic lipids facilitate membrane development and protochlorophyllide biosynthesis in etioplasts2023

    • Author(s)
      Yoshihara Akiko、Kobayashi Keiko、Nagata Noriko、Fujii Sho、Wada Hajime、Kobayashi Koichi
    • Journal Title

      Plant Physiology

      Volume: 194 Issue: 3 Pages: 1692-1704

    • DOI

      10.1093/plphys/kiad604

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Aerial roots of the leafless epiphytic orchid Taeniophyllum are specialized for performing crassulacean acid metabolism photosynthesis.2023

    • Author(s)
      Suetsugu K, Sugita R, Yoshihara A, Okada H, Akita K, Nagata N, Tanoi K, Kobayashi K
    • Journal Title

      New Phytol.

      Volume: 238 Issue: 3 Pages: 932-937

    • DOI

      10.1111/nph.18812

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] An improved fixative method for transmission electron microscopy observation of organelles in Arabidopsis seeds.2022

    • Author(s)
      T. Honda, M. Kobayashi, K. Kobayashi, K. Akita, N. Nagata
    • Journal Title

      J. Jpn. Women’s Univ. Fac. Sci.

      Volume: 30 Pages: 1-5

    • Related Report
      2022 Research-status Report
    • Open Access
  • [Journal Article] Crucial importance of length of fatty-acyl chains bound to the sn-2 position of phosphatidylglycerol for growth and photosynthesis of Synechocystis sp. PCC 68032022

    • Author(s)
      Kaichiro Endoa, Masato Abe, Nobumasa Kawanishi, Haruhiko Jimbo, Koichi Kobayashi, Tomoko Suzuki, Noriko Nagata, Hideto Miyoshi, Hajime Wada
    • Journal Title

      BBA - Molecular and Cell Biology of Lipids

      Volume: 1867 Issue: 7 Pages: 159158-159164

    • DOI

      10.1016/j.bbalip.2022.159158

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Smooth Loop-Like Mitochondrial Nucleus in the Primitive Red Alga <i>Cyanidioschyzon merolae</i> Revealed by Drying Treatment2021

    • Author(s)
      Kuroiwa, T., Yagisawa, F., Fujiwara, T., Misumi, O., Nagata, N., Imoto, Y., Yoshida, Y., Mogi, Y., Miyagishima, SY. and Kuroiwa, H.
    • Journal Title

      CYTOLOGIA

      Volume: 86 Issue: 1 Pages: 89-96

    • DOI

      10.1508/cytologia.86.89

    • NAID

      130008002729

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2021-03-25
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fertile <i>Arabidopsis cyp704b1</i> mutant, defective in sporopollenin biosynthesis, has a normal pollen coat and lipidic organelles in the tapetum2021

    • Author(s)
      Kobayashi Keiko、Akita Kae、Suzuki Masashi、Ohta Daisaku、Nagata Noriko
    • Journal Title

      Plant Biotechnology

      Volume: 38 Issue: 1 Pages: 109-116

    • DOI

      10.5511/plantbiotechnology.20.1214b

    • NAID

      130008002863

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2021-03-25
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Existence of giant mitochondria-containing sheet structures lacking cristae and matrix in etiolated cotyledon of Arabidopsis thaliana2021

    • Author(s)
      Fukushima, S., Akita, K., Takagi, T., Kobayashi, K., Moritoki, N., Arimura, S., Kuroiwa, H., Kuroiwa, T. and Nagata, N.
    • Journal Title

      Protoplasma

      Volume: 259 Issue: 3 Pages: 731-742

    • DOI

      10.1007/s00709-021-01696-0

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plastid anionic lipids are essential for the development of both photosynthetic and non-photosynthetic organs in Arabidopsis thaliana2021

    • Author(s)
      Akiko Yoshihara, Noriko Nagata, Hajime Wada, Koichi Kobayashi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 9 Pages: 4860-4860

    • DOI

      10.3390/ijms22094860

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen.2021

    • Author(s)
      K. Akita, T. Takagi, K. Kobayashi, K. Kuchitsu, T. Kuroiwa, N. Nagata
    • Journal Title

      Protoplasma

      Volume: 258 Issue: 1 Pages: 129-138

    • DOI

      10.1007/s00709-020-01557-2

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mitotic Karyotype of the Primitive Red Alga <i>Cyanidioschyzon merolae</i> 10D2020

    • Author(s)
      T. Kuroiwa, F. Yagisawa, T. Fujiwara, Y. Inui, T.M. Matsunaga, S. Katoi, S. Matsunaga, N. Nagata, Y. Imoto, H. Kuroiwa
    • Journal Title

      CYTOLOGIA

      Volume: 85 Issue: 2 Pages: 107-113

    • DOI

      10.1508/cytologia.85.107

    • NAID

      130007864023

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2020-06-25
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evolutionary significance of the ring-like plastid nucleus in the primitive red alga Cyanidioschyzon merolae as revealed by drying.2020

    • Author(s)
      T. Kuroiwa, M. Ohnuma, Y. Imoto, F. Yagisawa, O. Misumi, N. Nagata, H. Kuroiwa
    • Journal Title

      Protoplasma

      Volume: 257 Issue: 4 Pages: 1069-1078

    • DOI

      10.1007/s00709-020-01496-y

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] 連続切片SEM法により広がる植物オルガネラの世界2020

    • Author(s)
      永田典子
    • Journal Title

      Plant Morphology

      Volume: 32 Pages: 11-17

    • NAID

      130008006492

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] ステロール過剰蓄積変異体における花粉形成過程の超微構造学的解析2023

    • Author(s)
      小林啓子、秋田佳恵、 鈴木優志、島田貴士、永田典子
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2023 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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