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Taxonomic studies on novel discobids for more precise understanding of their diversity and the form ancestral eukaryotic cell.

Research Project

Project/Area Number 20K06792
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

Yabuki Akinori  国立研究開発法人海洋研究開発機構, 地球環境部門(海洋生物環境影響研究センター), グループリーダー (20711104)

Co-Investigator(Kenkyū-buntansha) 白鳥 峻志  筑波大学, 生命環境系, 助教 (70800621)
石田 健一郎  筑波大学, 生命環境系, 教授 (30282198)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords系統分類 / 多様性 / 微細構造 / 原生生物 / ディスコーバ / ユーグレノゾア / 新種記載 / ミトコンドリア / 新規遺伝子 / 初期進化 / 新規系統群 / ミトコンドリアゲノム / RNA編集 / 分子系統解析 / 高次分類 / 祖先的真核生物 / 真核微生物 / 細胞構造
Outline of Research at the Start

多様性の理解が不十分な真核微生物分類群の1つであるディスコーバに着目し、新規系統の探索を含む系統分類学的な研究を実施する。自然試料中より複数の新規ディスコーバ生物を探索し培養株として確立する。確立した培養株、および既に確立済みの培養株を用いた光学・電子顕微鏡観察による形態観察と超並列シーケンサーによる大規模配列の収集・解析等を行い、そこから記載等の分類学的処置を施す。さらにゲノムや各種代謝経路の理解と他生物との共培養実験等を通じて真核生物の成立や初期進化を類推する。

Outline of Final Research Achievements

The strain YPF-PP, which was one of the main targets in this research project, was identified as an undescribed species belonging to a novel subphylum in Discoba by molecular analyses and morphological observation. The strain YPF1504 was described as a new species in Apusomonadida, which was also one of the early branching eukaryotic lineages. It had been suggested as a member of Discoba in the initial observation, but it tuned out that it was an apusomonad. The large-scale phylogenetic analysis was conducted to understand the phylogenetic position of Diuscoba as well as the whole eukaryotic relationship, and based on this result, a novel eukaryotic assembly was proposed. A novel narnavirus was also detected and reported from the one protist strain that was established in this research project.

Academic Significance and Societal Importance of the Research Achievements

確立した培養株を用いた系統分類学的研究から、人類および地球という惑星における資源・資産である生物多様性に関する情報を更新する成果を創出することができた。特に、YPF-PP株に関する研究で把握した鞭毛周辺構造とその近縁生物種との比較からは、それらの特徴が真核生物進化の初期から存在していた可能性を見出した。これらの知見は、地球史における一大未解明事案である真核生物の初期進化、特に祖先真核細胞の姿形に迫る成果となっている。そこから、生物の多様性や進化のより正確な理解に貢献するだけでなく、真核細胞が元来有していた機能に関する理解の深化にも貢献するに至った。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (11 results)

All 2024 2023 2022 2021 2020

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] The copy number of the eukaryotic rRNA gene can be counted comprehensively2024

    • Author(s)
      Yabuki Akinori、Hoshino Tatsuhiko、Nakamura Tamiko、Mizuno Keiko
    • Journal Title

      MicrobiologyOpen

      Volume: 13 Issue: 2 Pages: 1-5

    • DOI

      10.1002/mbo3.1399

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The First Identification of a Narnavirus in Bigyra, a Marine Protist2023

    • Author(s)
      Chiba Yuto、Yabuki Akinori、Takaki Yoshihiro、Nunoura Takuro、Urayama Syun-ichi、Hagiwara Daisuke
    • Journal Title

      Microbes and Environments

      Volume: 38 Issue: 1 Pages: n/a

    • DOI

      10.1264/jsme2.ME22077

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Podomonas kaiyoae n. sp., a novel apusomonad growing axenically2022

    • Author(s)
      Yabuki Akinori、Tame Akihiro、Mizuno Keiko
    • Journal Title

      Journal of Eukaryotic Microbiology

      Volume: 70 Issue: 2

    • DOI

      10.1111/jeu.12946

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microheliella maris possesses the most gene-rich mitochondrial genome in Diaphoretickes2022

    • Author(s)
      Yazaki Euki、Yabuki Akinori、Nishimura Yuki、Shiratori Takashi、Hashimoto Tetsuo、Inagaki Yuji
    • Journal Title

      Frontiers in Ecology and Evolution

      Volume: 10 Pages: 1030570-1030570

    • DOI

      10.3389/fevo.2022.1030570

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 多様性と存在量を同時に推定する環境DNA解析手法の提案2024

    • Author(s)
      矢吹彬憲,星野辰彦,中村多実子,水野恵子
    • Organizer
      日本藻類学会第48回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 寄生性原生生物アセトスポラ類より新たに見出されたRNA編集とその進化2023

    • Author(s)
      矢吹彬憲, 藤井千早, 矢﨑裕規, 多米晃弘, 水野恵子, 大林由美子, 高尾祥丈
    • Organizer
      日本原生生物学会 日本寄生虫学会東日本支部 日本衛生動物学会東日本支部 3 学会合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Mitochondrial RNA editing in ascetosporean amoebae2023

    • Author(s)
      Akinori Yabuki, Chihaya Fujii, Euki Yazaki, Akihiro Tame, Keiko Mizuno, Yumiko Obayashi and Yoshitake Takao
    • Organizer
      IX European Congress of Protistology & Annual Congress of the International Society of Protistologists (ECOP/ISOP 2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 真核生物の大系統と初期細胞像の理解2022

    • Author(s)
      矢吹彬憲
    • Organizer
      国立環境研究所 所内セミナー
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 新規アセトスポラ綱原生生物が持つミトコンドリアRNA編集2021

    • Author(s)
      矢吹彬憲,藤井千早,大林由美子,高尾祥丈
    • Organizer
      第5回日本共生生物学会
    • Related Report
      2021 Research-status Report
  • [Presentation] Expanding Cryptista, illuminated by the study of Microheliella maris.2020

    • Author(s)
      Akinori Yabuki
    • Organizer
      ESOP 2
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 原生生物学事典2023

    • Author(s)
      矢﨑 裕規・新倉 保・猪飼 桂・矢吹 彬憲・永宗 喜三郎・松崎 素道・白鳥 峻志・島野 智之・小林 富美惠(編)
    • Total Pages
      452
    • Publisher
      朝倉書店
    • ISBN
      9784254171815
    • Related Report
      2023 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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