Molecular pathology evaluation method of PD-L1/PD-L2 focusing on structural and splicing abnormalities
| Project/Area Number |
20K07399
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49020:Human pathology-related
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| Research Institution | Japanese Foundation for Cancer Research |
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Principal Investigator |
SAKATA Seiji 公益財団法人がん研究会, がん研究所 分子標的病理プロジェクト, 研究員 (00617433)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | バイオマーカー |
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| Outline of Research at the Start |
本研究の目的は、免疫チェックポイント阻害薬の治療効果マーカーとなる分子病理学的診断法を確立させ、臨床応用すること、である。Programed cell death-ligand (PD-L) 1遺伝子の3´非翻訳領域の変異を伴う遺伝子構造異常や分泌型PD-L1スプライシングバリアント、PD-L1以外の免疫チェックポイント分子の発現、浸潤する炎症細胞などを評価し、治療効果との関連を検討する。
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| Outline of Final Research Achievements |
Targeted capture sequencing data of lymphoma specimens with high PD-L1 expression, their PD-L1 expression patterns by immunohistochemistry, and FISH findings were examined in detail. Samples in which PD-L1 genomic structural abnormalities were detected by targeted capture sequencing had a high rate of abnormal signal patterns by FISH. Almost all of the specimens that showed an abnormal expression pattern of PD-L1 by immunohistochemistry showed PD-L1 genomic structural abnormalities. Combined search of immunohistochemistry and FISH results showed a high detection rate of PD-L1 genomic structural aberrations.
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| Academic Significance and Societal Importance of the Research Achievements |
ゲノム構造異常に伴うPD-L1遺伝子の3´非翻訳領域の変異の検索には、一般的にターゲットキャプチャーシーケンスでの検索が必要となるが、経験やスキルが必要で費用が高く、利用可能な機関が限られる。免疫染色およびFISHはより簡便に行うことができ、検体量の少ない場合でも検索可能なため、ゲノム構造異常に伴うPD-L1遺伝子の3´非翻訳領域の変異の検索に免疫染色とFISHが有用であったことは社会的に意義がある。
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Report
(4 results)
Research Products
(4 results)
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[Presentation] MET amplification in head and neck cancer: the frequency and predictive value for immune checkpoint inhibitor therapy2023
Author(s)
Kenji Nakano, Seiji Sakata, Yasuyoshi Sato, Xiaofei Wang, Naomi Hayashi, Hirotaka Suto, Ryosuke Oki, Naoki Fukuda, Tetsuya Urasaki, Makiko Ono, Junichi Tomomatsu, Hiroki Mitani and Shunji Takahashi
Organizer
日本臨床腫瘍学会
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