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Analysis of virulence genes in Escherichia albertii from HUS cases.

Research Project

Project/Area Number 20K07506
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49050:Bacteriology-related
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Iyoda Sunao  国立感染症研究所, 細菌第一部, 室長 (70300928)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsE. albertii / pch / 発現制御 / HUS
Outline of Research at the Start

HUS患者便から分離したStx2f産生性のEscherichia albertii(EA)株などを用いて、EAに存在する3種類の3型蛋白質輸送装置(T3SS)すなわち、LEE、ETT2、べん毛の発現制御機構の解明と新規T3エフェクターの同定を目指す。さらに、Stx2のサブタイプとして近年重症例に散見されるStx2fの発現制御機構および細胞毒性について、上記のT3SSの機能解析と共に培養細胞レベルで解析する計画である。

Outline of Final Research Achievements

Shiga toxin-producing Escherichia albertii (EA), which we isolated from a patient with haemolytic uraemic syndrome, harbour LEE, a group of virulence genes required for intimate adhesion to host cells. As important regulators of LEE expression in enterohemorrhagic E. coli ( EHEC), we previously identified the pch gene cluster (pchA-E). This study shows that pchB and a novel pch, pchF, both play important roles in LEE gene expression in EA, with pchF being the most important regulator of LEE expression in EA.

Academic Significance and Societal Importance of the Research Achievements

EHEC感染による重症例である溶血性尿毒症症候群(HUS)を発症する年齢は10歳までの小児に最も多い。EHECと近縁であるが大腸菌とは異なる種に分類されるEscherichia albertii(EA)は、これまでHUSを発症したケースは報告されていなかった。我々はHUS発症患者から志賀毒素産生性のEAを分離同定し、その病原性に重要と考えられる細胞接着性にかかわる遺伝子群LEEの発現に必須な遺伝子を同定した。本研究の成果はEHECおよびEAが保有する病原性遺伝子群の発現制御機構の解明に向けた重要な知見となり、EAの病原性を理解する上で重要な知見になるものと考えられる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (30 results)

All 2024 2023 2022 2021 2020 Other

All Journal Article (22 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 22 results,  Open Access: 18 results) Presentation (7 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Complete genome sequence of eight LEE-negative Shiga toxin-producing <i>Escherichia coli</i> strains isolated from patients with hemolytic-uremic syndrome2024

    • Author(s)
      Kubomura Akiko、Lee Kenichi、Ohnishi Makoto、Iyoda Sunao、Akeda Yukihiro、Working Group EHEC
    • Journal Title

      Microbiology Resource Announcements

      Volume: 13 Issue: 1 Pages: 1-4

    • DOI

      10.1128/mra.00591-23

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Combined usage of serodiagnosis and O antigen typing to isolate Shiga toxin-producing Escherichia coli O76:H7 from a hemolytic uremic syndrome case and genomic insights from the isolate2024

    • Author(s)
      Kenichi Lee, Atsushi Iguchi, Chikako Terano, Hiroshi Hataya, Junko Isobe, Kazuko Seto, Nozomi Ishijima, Yukihiro Akeda, Makoto Ohnishi, Sunao Iyoda, EHEC Working Group in Japan
    • Journal Title

      Microbiology Spectrum

      Volume: 12 Issue: 1

    • DOI

      10.1128/spectrum.02355-23

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Atypical diarrhoeagenic <i>Escherichia coli</i> in milk related to a large foodborne outbreak2023

    • Author(s)
      Hirose Shouhei、Ohya Kenji、Yoshinari Tomoya、Ohnishi Takahiro、Mizukami Katsumi、Suzuki Tomikatsu、Takinami Kenji、Suzuki Takayoshi、Lee Kenichi、Iyoda Sunao、Akeda Yukihiro、Yahata Yuichiro、Tsuchihashi Yuuki、Sunagawa Tomimasa、Hara-Kudo Yukiko
    • Journal Title

      Epidemiology and Infection

      Volume: 151

    • DOI

      10.1017/s0950268823001395

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Global population structure, genomic diversity and carbohydrate fermentation characteristics of clonal complex 119 (CC119), an understudied Shiga toxin-producing E. coli (STEC) lineage including O165:H25 and O172:H252023

    • Author(s)
      Nakamura Keiji、Seto Kazuko、Lee Kenichi、Ooka Tadasuke、Gotoh Yasuhiro、Taniguchi Itsuki、Ogura Yoshitoshi、Mainil Jacques Georges、Pi?rard Denis、Harada Tetsuya、Etoh Yoshiki、Ueda Saori、Hamasaki Mitsuhiro、Isobe Junko、Kimata Keiko、Narimatsu Hiroshi、Yatsuyanagi Jun、Ohnishi Makoto、Iyoda Sunao、Hayashi Tetsuya
    • Journal Title

      Microbial Genomics

      Volume: 9 Issue: 3 Pages: 000959-000959

    • DOI

      10.1099/mgen.0.000959

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Alteration of a Shiga toxin-encoding phage associated with a change in toxin production level and disease severity in Escherichia coli2023

    • Author(s)
      Miyata Tatsuya、Taniguchi Itsuki、Nakamura Keiji、Gotoh Yasuhiro、Yoshimura Dai、Itoh Takehiko、Hirai Shinichiro、Yokoyama Eiji、Ohnishi Makoto、Iyoda Sunao、Ogura Yoshitoshi、Hayashi Tetsuya
    • Journal Title

      Microbial Genomics

      Volume: 9 Issue: 2

    • DOI

      10.1099/mgen.0.000935

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real-time PCR assays to detect 10 Shiga toxin subtype (Stx1a, Stx1c, Stx1d, Stx2a, Stx2b, Stx2c, Stx2d, Stx2e, Stx2f, and Stx2g) genes2023

    • Author(s)
      Harada Tetsuya、Wakabayashi Yuki、Seto Kazuko、Lee Kenichi、Iyoda Sunao、Kawatsu Kentaro
    • Journal Title

      Diagnostic Microbiology and Infectious Disease

      Volume: 105 Issue: 3 Pages: 115874-115874

    • DOI

      10.1016/j.diagmicrobio.2022.115874

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of constant cell elongation and Sfm pili synthesis in <i>Escherichia coli</i> via two active forms of <scp>FimZ</scp> orphan response regulator2022

    • Author(s)
      Ogawa Ayano、Kojima Fumika、Miyake Yukari、Yoshimura Miho、Ishijima Nozomi、Iyoda Sunao、Sekine Yasuhiko、Yamanaka Yuki、Yamamoto Kaneyoshi
    • Journal Title

      Genes to Cells

      Volume: 27 Issue: 11 Pages: 657-674

    • DOI

      10.1111/gtc.12982

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multijurisdictional Outbreak of Enterohemorrhagic Escherichia coli O157 Caused by Consumption of Ready-to-Eat Grilled Skewered Meat in Niigata, Japan2022

    • Author(s)
      Ryosuke Sato, Yuichiro Yahata, Hiroyuki Taira, Tetsuya Saito, Teruyuki Ishii, Satoru Yamazaki, Kazunari Yamamoto, Ryoko Kikuchi, Hidemasa Izumiya, Sunao Iyoda, Makoto Ohnishi, Yoshiki Takahashi
    • Journal Title

      Foodborne Pathogens and Disease

      Volume: - Issue: 6 Pages: 400-407

    • DOI

      10.1089/fpd.2021.0083

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Subtilase cytotoxin from Shiga-toxigenic Escherichia coli impairs the inflammasome and exacerbates enteropathogenic bacterial infection.2022

    • Author(s)
      Tsutsuki Hiroyasu, Zhang Tianli, Yahiro Kinnosuke, Ono Katsuhiko, Fujiwara Yukio, Iyoda Sunao, Wei Fan-Yan, Monde Kazuaki, Seto Kazuko, Ohnishi Makoto, Oshiumi Hiroyuki, Akaike Takaaki, Sawa Tomohiro.
    • Journal Title

      iScience

      Volume: 25 Issue: 4 Pages: 104050-104050

    • DOI

      10.1016/j.isci.2022.104050

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The global population structure and evolutionary history of the acquisition of major virulence factor-encoding genetic elements in Shiga toxin-producing Escherichia coli O121:H192021

    • Author(s)
      Nishida Ruriko、Nakamura Keiji、Taniguchi Itsuki、et al.
    • Journal Title

      Microbial Genomics

      Volume: 7 Issue: 12 Pages: 000716-000716

    • DOI

      10.1099/mgen.0.000716

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A novel endoplasmic stress mediator, Kelch domain containing 7B (KLHDC7B), increased Harakiri (HRK) in the SubAB-induced apoptosis signaling pathway2021

    • Author(s)
      Yahiro Kinnosuke、Ogura Kohei、Tsutsuki Hiroyasu、Iyoda Sunao、Ohnishi Makoto、Moss Joel
    • Journal Title

      Cell Death Discovery

      Volume: 7 Issue: 1 Pages: 360-360

    • DOI

      10.1038/s41420-021-00753-0

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An outbreak of food poisoning due to <i>Escherichia coli</i> serotype O7:H4 carrying <i>astA</i> for enteroaggregative <i>E. coli</i> heat-stable enterotoxin1 (EAST1)2021

    • Author(s)
      Kashima K.、Sato M.、Osaka Y.、Sakakida N.、Kando S.、Ohtsuka K.、Doi R.、Chiba Y.、Takase S.、Fujiwara A.、Shimada S.、Ishii R.、Mizokoshi A.、Takano M.、Lee K.、Iyoda S.、Honda A.
    • Journal Title

      Epidemiology and Infection

      Volume: 149

    • DOI

      10.1017/s0950268821002338

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Collateral effects of deletion of nlpD on rpoS and rpoS-dependent genes2021

    • Author(s)
      Tsunoi Manami、Iyoda Sunao、Iwase Tadayuki
    • Journal Title

      Journal of Clinical Investigation

      Volume: 131 Issue: 18

    • DOI

      10.1172/jci152693

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Stabilizing Genetically Unstable Simple Sequence Repeats in the Campylobacter jejuni Genome by Multiplex Genome Editing: a Reliable Approach for Delineating Multiple Phase-Variable Genes2021

    • Author(s)
      Yamamoto Shouji、Iyoda Sunao、Ohnishi Makoto
    • Journal Title

      mBio

      Volume: 12 Issue: 4

    • DOI

      10.1128/mbio.01401-21

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] RNA‐binding protein Hfq downregulates locus of enterocyte effacement‐encoded regulators independent of small regulatory RNA in enterohemorrhagic <i>Escherichia coli</i>2021

    • Author(s)
      Sudo Naoki、Lee Kenichi、Sekine Yasuhiko、Ohnishi Makoto、Iyoda Sunao
    • Journal Title

      Molecular Microbiology

      Volume: 117 Issue: 1 Pages: 86-101

    • DOI

      10.1111/mmi.14799

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diversity of the Tellurite Resistance Gene Operon in Escherichia coli2021

    • Author(s)
      Nguyen Thi Thu Huong、Kikuchi Taisei、Tokunaga Tadaaki、Iyoda Sunao、Iguchi Atsushi
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 681175-681175

    • DOI

      10.3389/fmicb.2021.681175

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Whole-Genome Sequencing of Shiga Toxin?Producing Escherichia coli OX18 from a Fatal Hemolytic Uremic Syndrome Case2021

    • Author(s)
      Lee Kenichi、Iguchi Atsushi、Uda Kazuhiro、Matsumura Sohshi、Miyairi Isao、Ishikura Kenji、Ohnishi Makoto、Seto Junji、Ishikawa Kanako、Konishi Noriko、Obata Hiromi、Furukawa Ichiro、Nagaoka Hiromi、Morinushi Hirotaka、Hama Natsuki、Nomoto Ryohei、Nakajima Hiroshi、Kariya Hideaki、Hamasaki Mitsuhiro、Iyoda Sunao
    • Journal Title

      Emerging Infectious Diseases

      Volume: 27 Issue: 5 Pages: 1509-1512

    • DOI

      10.3201/eid2705.204162

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiple-Locus Variable-Number Tandem Repeat Analysis Scheme for Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i>: Focus on Serogroups O103, O121, O145, O165, and O912020

    • Author(s)
      Izumiya Hidemasa、Lee Kenichi、Ishijima Nozomi、Iyoda Sunao、Ohnishi Makoto
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 73 Issue: 6 Pages: 481-490

    • DOI

      10.7883/yoken.JJID.2020.095

    • NAID

      130007942241

    • ISSN
      1344-6304, 1884-2836
    • Year and Date
      2020-11-30
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Distribution of Novel Og Types in Shiga Toxin-Producing Escherichia coli Isolated from Healthy Cattle2020

    • Author(s)
      Nguyen Thi Thu Huong、Iguchi Atsushi、Ohata Ritsuko、Kawai Hisahiro、Ooka Tadasuke、Nakajima Hiroshi、Iyoda Sunao
    • Journal Title

      Journal of Clinical Microbiology

      Volume: 59 Issue: 3

    • DOI

      10.1128/jcm.02624-20

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Subtilase cytotoxin induces a novel form of Lipocalin 2, which promotes Shiga-toxigenic Escherichia coli survival.2020

    • Author(s)
      Yahiro, K., Ogura, K., Goto, Y., Iyoda, S., Kobayashi, T., Takeuchi, H., Ohnishi, M., Moss, J.
    • Journal Title

      Scientific Reports.

      Volume: 10 Issue: 1 Pages: 18943-18943

    • DOI

      10.1038/s41598-020-76027-z

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Additional Og-Typing PCR Techniques Targeting Escherichia coli-Novel and Shigella-Unique O-Antigen Biosynthesis Gene Clusters2020

    • Author(s)
      Iguchi Atsushi、Nishii Hironobu、Seto Kazuko、Mitobe Jiro、Lee Kenichi、Konishi Noriko、Obata Hiromi、Kikuchi Taisei、Iyoda Sunao
    • Journal Title

      Journal of Clinical Microbiology

      Volume: 58 Issue: 11

    • DOI

      10.1128/jcm.01493-20

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Global distribution of epidemic-related Shiga toxin 2 encoding phages among enteroaggregative Escherichia coli2020

    • Author(s)
      Kimata Keiko、Lee Kenichi、Watahiki Masanori、Isobe Junko、Ohnishi Makoto、Iyoda Sunao
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 11738-11738

    • DOI

      10.1038/s41598-020-68462-9

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 重症例由来腸管出血性大腸菌の細菌学的特徴について2023

    • Author(s)
      伊豫田 淳,李 謙一
    • Organizer
      第 96 回日本細菌学会総会 シンポジウム S5 食中毒研究の最前線
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] マルチプレックスゲノム編集を用いてCampylobacter jejuniの表現型を制御する2022

    • Author(s)
      山本章治,伊豫田淳,大西 真
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Research-status Report
  • [Presentation] 大腸菌 O103:H2 国内分離株とデータベース株を用いた高精度系統 解析及び完全長配列決定株のゲノム構造比較2022

    • Author(s)
      谷口愛樹,中村佳司,後藤恭宏,李謙一,大岡唯祐,小椋義俊,大西真,伊豫田淳,林哲也
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Research-status Report
  • [Presentation] 大腸菌における亜テルル酸耐性オペロンの多様性2022

    • Author(s)
      井口純,Thi Thu Huong Nguyen,菊地泰生,伊豫田淳
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Research-status Report
  • [Presentation] 完全長配列を用いたEHEC O157 clade 8のゲノムと Stx2ファー ジの多様性解析2022

    • Author(s)
      宮田達弥,谷口愛樹,中村 佳司,後藤恭宏,小椋義俊,大西真,伊豫田淳,林哲也
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Research-status Report
  • [Presentation] SubABにより発現するKLHDC7BはHarakiriを誘導し細胞死を引き起こす2022

    • Author(s)
      八尋錦之助,小倉康平,津々木 博康,伊豫田淳,大西真
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Research-status Report
  • [Presentation] 腸管出血性大腸菌O157における変異型亜テルル酸耐性遺伝子tehAによる耐性増強2022

    • Author(s)
      赤坂龍矢,松本裕子,李謙一,小泉充正,佐藤寿夫,明田幸宏,大西真,伊豫田淳
    • Organizer
      第95回日本細菌学会総会
    • Related Report
      2021 Research-status Report
  • [Remarks] 腸管出血性大腸菌 (EHEC) 検査・診断マニュアル

    • URL

      https://www.niid.go.jp/niid/images/lab-manual/EHEC20210907.pdf

    • Related Report
      2021 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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