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Various studies using human iPS cell-derived melanocyte

Research Project

Project/Area Number 20K08660
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 53050:Dermatology-related
Research InstitutionTohoku Medical and Pharmaceutical University

Principal Investigator

Kawakami Tamihiro  東北医科薬科大学, 医学部, 教授 (20297659)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsメラノサイト / 白斑 / 悪性黒色腫 / 皮膚色素細胞 / iPS細胞 / 再生医療 / 美白化粧品 / 脱色素斑 / 美白物質 / ケラチノサイト
Outline of Research at the Start

ヒトiPS細胞由来メラノサイトを独自の条件設定で、効率よく分化・増殖させ、大量産生に成功した(特許取得)。本研究は、この細胞のさまざまな臨床応用へのステップである。
①メラノサイトの欠如・機能不全疾患である尋常性白斑や脱色素斑への移植を含めた再生医療。
②メラノサイトが癌化した悪性黒色腫の機序解明に使用し、重要因子の発見と有意義な治療法の開発。
③美白化粧品の主成分である様々な物質のメラノサイトへの効果を検証する美白化粧品開発に利用。

Outline of Final Research Achievements

Adding Rho-associated protein kinase (ROCK) inhibitor to the culture medium dramatically increased the yield of pure melanocytes. RT-qPCR analysis of vitiligo-derived primary melanocytes revealed significantly decreased mRNA levels of microphthalmia-associated transcription factor (MITF) compared to normal melanocytes. In contrast, mRNA expression levels of stem cell factor (SCF) were significantly higher in co-culture of vitiligo-derived primary melanocytes and normal keratinocytes than in co-culture of normal melanocytes and normal keratinocyte. RT-qPCR analysis of melanocyte related adhesion molecules in vitiligo-derived primary melanocytes revealed significantly decreased mRNA levels compared to normal melanocytes. Based on these findings, we suggested that human primary melanocytes from non-depigmented skin in a patient with non-segmental vitiligo could be not normal melanocytes.

Academic Significance and Societal Importance of the Research Achievements

本研究は、ヒトiPS細胞由来メラノサイトを使用して、白斑・悪性黒色腫など関連疾患を検討することである。ヒトiPS細胞をadvanced iDMMで培養することで、2週間で成熟ヒトメラノサイトが完成できる。この細胞を使用することで、白斑におけるメラノサイトの脆弱性や悪性黒色腫におけるメラノサイトの発癌性を解明できる。また、白斑治療では、欠損や機能不全になったメラノサイト病変部へ大量の良質メラノサイトを供給できる、悪性黒色腫治療では、患者本人からヒトiPS細胞由来メラノサイトを作成し、試験管レベルでどの免疫チェックポイント阻害薬がどの段階で有効かが把握でき、より適切な治療が確立できる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (30 results)

All 2022 2021 2020

All Journal Article (15 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 15 results,  Open Access: 15 results) Presentation (14 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Surgical procedures and innovative approaches for vitiligo regenerative treatment and melanocytorrhagy.2022

    • Author(s)
      Kawakami T
    • Journal Title

      Journal of Dermatology

      Volume: 49 Issue: 4 Pages: 391-401

    • DOI

      10.1111/1346-8138.16316

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of adhesion molecules and basic fibroblast growth factor 2 in non-segmental vitiligo-derived primary melanocytes.2022

    • Author(s)
      Dong Y, Kawakami T, Komatsu T.
    • Journal Title

      Journal of Dermatological Science

      Volume: 108 Issue: 2 Pages: 109-111

    • DOI

      10.1016/j.jdermsci.2022.10.005

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Early add-on administration of mepolizumab and intravenous immunoglobulin effective in treating eosinophilic granulomatosis with polyangiitis.2021

    • Author(s)
      Ikeda T, Komatsu T, Yokoyama K, Takahashi K, Kawakami T.
    • Journal Title

      J Dermatol

      Volume: 48 Issue: 4 Pages: 529-532

    • DOI

      10.1111/1346-8138.15709

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anti‐phosphatidylserine/prothrombin complex antibodies in patients with cutaneous vasculitis: Possible involvement in the pathogenesis2021

    • Author(s)
      Kawakami Tamihiro、Tamura Yuto、Dong Yupeng、Yoshinari Miku、Nishibata Yuka、Masuda Sakiko、Tomaru Utano、Ishizu Akihiro
    • Journal Title

      The Journal of Dermatology

      Volume: 48 Issue: 5 Pages: 703-706

    • DOI

      10.1111/1346-8138.15810

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinical and immunological findings in 55 cases of anti-laminin-332-type mucous membrane pemphigoid.2021

    • Author(s)
      Li X, Qian H, Natsuaki Y, Koga H, Kawakami T, Tateishi C, Tsuruta D, Ishii N, Hashimoto T.
    • Journal Title

      Br J Dermatol

      Volume: 185 Issue: 2 Pages: 449-451

    • DOI

      10.1111/bjd.20099

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The relationship between anti-phosphatidylserine/prothrombin complex IgM antibodies and cutaneous ulcers in patients with cutaneous vasculitis.2021

    • Author(s)
      Kawakami T, Okiyama N, Kodera M, Seishima M, Yamaguchi Y.
    • Journal Title

      J Dermatol

      Volume: 48 Issue: 9 Pages: 1457-1458

    • DOI

      10.1111/1346-8138.16014

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The second study of clinical and immunological findings in anti-laminin 332-type mucous membrane pemphigoid examined at Kurume University - Diagnosis criteria suggested by summary of 133 cases.2021

    • Author(s)
      Qian H, Natsuaki Y, Koga H, Kawakami T, Tateishi C, Tsuruta D, Ishii N, Li X, Hashimoto T.
    • Journal Title

      Frontiers in Immunology

      Volume: 26 Pages: 771766-771766

    • DOI

      10.3389/fimmu.2021.771766

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Elevated Myeloperoxidase-DNA Complex Levels in Sera of Patients with IgA Vasculitis2021

    • Author(s)
      Takeuchi Sora、Kawakami Tamihiro、Okano Tatsuro、Shida Haruki、Nakazawa Daigo、Tomaru Utano、Ishizu Akihiro、Kadono Takafumi
    • Journal Title

      Pathobiology

      Volume: 89 Issue: 1 Pages: 23-28

    • DOI

      10.1159/000519869

    • NAID

      120007173221

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of co-culture of human induced pluripotent stem (iPS) cell-derived melanocytes and keratinocytes in vitro.2021

    • Author(s)
      Kawakami T, Komatsu T, Yokoyama K, Iwama E, Dong Y.
    • Journal Title

      J Dermatol.

      Volume: 48 Pages: 123-125

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relationship between lysosomal-associated membrane protein-2 and anti-phosphatidylserine/prothrombin complex antibody in pathogenesis of cutaneous vasculitis.2020

    • Author(s)
      Kawakami T, Kikuchi A, Miyabe C, Ikeda T, Takeuchi S, Tamura Y, Nishibata Y, Masuda S, Nakazawa D, Tomaru U, Ishizu A.
    • Journal Title

      Clin Exp Rheumatol.

      Volume: 38 Pages: 161-165

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinical characteristics and social productivity levels of patients with malignant rheumatoid arthritis based on a nationwide clinical database in Japan: annual survey from 2003 to 20132020

    • Author(s)
      Abe Yoshiyuki、Saeki Keigo、Dobashi Hiroaki、Kawakami Tamihiro、Hayashi Taichi、Kobayashi Masaki、Kaname Shinya、Harigai Masayoshi、Tamura Naoto
    • Journal Title

      Modern Rheumatology

      Volume: 31 Issue: 3 Pages: 1-8

    • DOI

      10.1080/14397595.2020.1795390

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Skin biopsies using dermoscopy for earlier diagnosis of intravasculer large B-cell lymphoma.2020

    • Author(s)
      Miyabe C, Nomura J, Ikeda T, Takahashi K, Kawakami T.
    • Journal Title

      J Dermatol.

      Volume: 47

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pityriasis lichenoides et varioliformis associated with anti-Ku positive refractory interstitial lung disease and dermatomyositis.2020

    • Author(s)
      Ikeda T, Shirota Y, Iwama E, Yokoyama K, Takahashi K, Kawakami T.
    • Journal Title

      J Dermatol.

      Volume: 47

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Wound, pressure ulcer and burn guidelines-1: Guidelines for wounds in general, second edition.2020

    • Author(s)
      Hasegawa M, Inoue Y, Kaneko S, Kanoh H, Shintani Y, Tsujita J, Fujita H, Motegi S, Pavoux AL, Asai J, Asano Y, Abe M, Amano M, Ikegami R, Ishii T, Isei T, Isogai Z, Ito T, Irisawa R, Iwata Y, Otsuka M, Omoto Y, Kato H, Kadono T, Kawakami T, et al.
    • Journal Title

      J Dermatol.

      Volume: 47 Pages: 807-833

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clinical practice guide for the treatment of perforating dermatosis.2020

    • Author(s)
      Kawakami T, Akiyama M, Ishida-Yamamoto A, Nakano H, Mitoma C, Yoneda K, Suga Y.
    • Journal Title

      J Dermatol.

      Volume: 47 Pages: 1374-1382

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Regulation of adhesion molecules and basic fibroblast growth factor 2 in non-segmental vitiligo-derived primary melanocytes.2022

    • Author(s)
      Kawakami T, Dong Y, Komatsu T.
    • Organizer
      The 31st Japanese Society for Pigment Cell Research
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 非分節型尋常性白斑患者の健常皮膚におけるメラノサイトの性状2022

    • Author(s)
      川上民裕、小松俊郎、董宇鵬
    • Organizer
      日本皮膚科学会東北六県合同地方会学術大会第398回例会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 尋常性白斑患者の健常皮膚メラノサイトは正常ではない2022

    • Author(s)
      川上民裕、董宇鵬
    • Organizer
      第40回日本美容皮膚科学会総会・学術大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 非分節型尋常性白斑患者の正常皮膚メラノサイトは正常ではない2022

    • Author(s)
      川上民裕、小松俊郎、董宇鵬
    • Organizer
      第4回日本白斑学会学術大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ニトロプルシドナトリウムによる白斑メラノサイトの増殖促進作用について2022

    • Author(s)
      安藤秀哉、横井一範、種村篤、川上民裕、市橋正光
    • Organizer
      第4回日本白斑学会学術大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 白斑の再生治療とiPS細胞2022

    • Author(s)
      川上民裕
    • Organizer
      第7回白斑白皮症研究会
    • Related Report
      2021 Research-status Report
  • [Presentation] Anti-phosphatidylserine/prothrombin complex antibodies in patients with cutaneous vasculitis: Possible involvement in the pathogenesis.2021

    • Author(s)
      Kawakami T, Tamura Y, Dong Y, Yoshinari M, Nishibata Y, Masuda S, Tomaru U, Ishizu A.
    • Organizer
      2021 Society for Investigative Dermatology Virtual Meeting.
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒトiPS細胞由来メラノサイトを使用した白斑治療戦略と美白化粧品の評価試験への応用2021

    • Author(s)
      川上民裕
    • Organizer
      第5回・第6回花王メラニン研究会成果報告会
    • Related Report
      2021 Research-status Report
  • [Presentation] 白斑の未来を見据えた再生治療 -メラノサイト脆弱性の克服-2021

    • Author(s)
      川上民裕
    • Organizer
      第3回日本白斑学会学術大会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 白斑メラノサイトと正常メラノサイトの相違点 -メラニン量が多い白斑患者由来メラノサイト-2021

    • Author(s)
      安藤秀哉、横井一範、種村篤、川上民裕、市橋正光
    • Organizer
      第3回日本白斑学会学術大会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Establishment of human iPS cell-derived melanocyte as potential source for melanocyte regeneration.2020

    • Author(s)
      Kawakami T.
    • Organizer
      The XXⅣInternational Pigment Cell Conference
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Dermatological perspective on eosinophilic granulomatosis with polyangiitis (EGPA), including the MIRRA study (mepolizumab, an anti-IL-5 antibody treatment). Mepolizumab EGPA seminar.2020

    • Author(s)
      Kawakami T.
    • Organizer
      The XXⅣ International Pigment Cell Conference
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Establishment of co-culture of human induced pluripotent stem (iPS) cell-derived melanocyte and keratinocytes in vitro.2020

    • Author(s)
      Kawakami T, Komatsu T, Yokoyama K, Ikeda T, Takahashi K, Dong Y.
    • Organizer
      The 45th Annual Meeting of the Japanese Society for Investigative Dermatology.
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] 今後の治療法 白斑の病態と治療2020

    • Author(s)
      川上民裕
    • Organizer
      第119回日本皮膚科学会総会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] 高分化度の多能性幹細胞由来細胞の製造方法2021

    • Inventor(s)
      川上民裕
    • Industrial Property Rights Holder
      川上民裕
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-004816
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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