Identification of VWF-HBD in vascular growth and interaction with an inhibitory VEGF isoform

• Project/Area Number: 20K08711
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 54010:Hematology and medical oncology-related
• Research Institution: Nagoya University
• Principal Investigator: Matsushita Tadashi 名古屋大学, 医学部附属病院, 教授 (30314008)
• Co-Investigator(Kenkyū-buntansha): 鈴木 伸明 名古屋大学, 医学部附属病院, 講師 (70637686) ; 菊地 良介 岐阜大学, 医学部附属病院, 臨床検査技師長 (30721435)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ECFC / VWF / VWD / Weibel-Palade body / DDAVP / Type 3 VWD / Platelet glycoprotein Ib / multimer / von Willebrand factor / von Willebrand disease / Type 3 / VEGF / angiogenesis / GRIb / Heparin / 血管新生 / von Willebrand facor / GPIb / heparin / vascular growth

Outline of Research at the Start

VWFは、そのヘパリン結合ドメインがGFに結合し、創傷治癒において血管新生を促進するという新しい機能が近年指摘されている。我々はVWFのヘパリン結合部位 (HBD)をin vitroでピンポイント同定しており、本研究ではこの結合部位のVWFの血管新生機能における重要性を検討、一部のVWD患者に見られる血管新生障害の原因としての「血管新生抑制型VEGF-Aアイソフォーム(VEGF-A165b)」の負の影響を検討することにより、血漿・内皮細胞由来のVWFがヘパリンへの結合を介して血管外マトリックスへ結合することにより、どのようにGFの血管新生を調節するか明らかにする。

Outline of Final Research Achievements

Type 3 VWD, the most severe form of von Willebrand disease (VWD), causes refractory bleeding that does not respond to replacement therapy. Angiodysplasia has been implicated in such bleeding, but the specific pathomechanism has not been elucidated. In this study, we focused on the angiogenic potential of ECMC derived from type 3 patients, and also examined the expression and intracellular profiles of mutant VWF in VWD type 3 patients with trace amount of VWF levels and a novel p.Gly2752Ser mutation. We analyzed the expression and intracellular dynamics of mutant VWF in patients with VWD type 3.

Academic Significance and Societal Importance of the Research Achievements

VWFは内皮細胞という再現の難しい培養細胞種から分泌され、またWPBという特徴的な小胞体に貯留され、刺激にて分泌されるという特徴を有する。ECFCは、血管内皮細胞環境を患者由来として直接構築できる特徴がある。本研究では従来のTransfectionによる発現検討と組み合わせ、優性遺伝形式を取るVWDにおけるDominant-negative effectの細胞学的な機構を詳細に検討を行うことができた。一方、正常アレル由来の発現を最適化すれば、VWF濃縮製剤を使用することなく治療が可能となるはずである。本研究で得られた最適な分泌刺激方法により新たなVWD治療の基盤モデルとなることが期待される。

Research Products

• [Journal Article] International consensus recommendations on the management of people with haemophilia B (2022)
  • Author(s): Hart Daniel P.、Matino Davide、Astermark Jan、Dolan Gerard、d’Oiron Roseline、Hermans Cedric、Jimenez-Yuste Victor、Linares Adriana、Matsushita Tadashi、McRae Simon、Ozelo Margareth C.、Platton Sean、Stafford Darrel、Sidonio Robert F.、Tiede Andreas
  • Journal Title: Therapeutic Advances in Hematology Volume: 13
  • DOI: 10.1177/20406207221085202
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] F9 mRNA splicing aberration due to a deep Intronic structural variation in a patient with moderate hemophilia B (2022)
  • Author(s): Odaira Koya、Kawashima Fumika、Tamura Shogo、Suzuki Nobuaki、Tokoro Mahiru、Hayakawa Yuri、Suzuki Atsuo、Kanematsu Takeshi、Okamoto Shuichi、Takagi Akira、Katsumi Akira、Matsushita Tadashi、Shima Midori、Nogami Keiji、Kojima Tetsuhito、Hayakawa Fumihiko
  • Journal Title: Thrombosis Research Volume: 213 Pages: 91-96
  • DOI: 10.1016/j.thromres.2022.03.010
  • Peer Reviewed / Open Access
• [Journal Article] Periosteum-derived podoplanin-expressing stromal cells regulate nascent vascularization during epiphyseal marrow development (2022)
  • Author(s): Tamura Shogo、Mukaide Masato、Katsuragi Yumi、Fujii Wataru、Odaira Koya、Suzuki Nobuaki、Tsukiji Nagaharu、Okamoto Shuichi、Suzuki Atsuo、Kanematsu Takeshi、Katsumi Akira、Takagi Akira、Ikeda Katsuhide、Ueyama Jun、Hirayama Masaaki、Suzuki-Inoue Katsue、Matsushita Tadashi、Kojima Tetsuhito、Hayakawa Fumihiko
  • Journal Title: Journal of Biological Chemistry Volume: 298 Issue: 5 Pages: 101833-101833
  • DOI: 10.1016/j.jbc.2022.101833
  • Peer Reviewed / Open Access
• [Journal Article] VWF-Gly2752Ser, a novel non-cysteine substitution variant in the CK domain, exhibits severe secretory impairment by hampering C-terminal dimer formation (2022)
  • Author(s): Okamoto S, Tamura S, Sanda N, Odaira K, Hayakawa Y, Mukaide M, Suzuki A, Kanematsu T, Hayakawa F, Katsumi A, Kiyoi H, Kojima T, Matsushita T, Suzuki N.
  • Journal Title: J Thromb Haemost Volume: 20 Issue: 8 Pages: 1784-1796
  • DOI: 10.1111/jth.15746
  • Peer Reviewed / Open Access
• [Journal Article] 【血液疾患のすべて】出血・血栓性疾患 von Willebrand病 (2022)
  • Author(s): 松下正
  • Journal Title: 日本医師会雑誌 Volume: 151巻特別1
• [Journal Article] Indoxyl Sulfate Activates NLRP3 Inflammasome to Induce Cardiac Contractile Dysfunction Accompanied by Myocardial Fibrosis and Hypertrophy (2022)
  • Author(s): Yamaguchi Kazutoshi、Yisireyili Maimaiti、Goto Sumie、Cheng Xian Wu、Nakayama Takayuki、Matsushita Tadashi、Niwa Toshimitsu、Murohara Toyoaki、Takeshita Kyosuke
  • Journal Title: Cardiovascular Toxicology Volume: 22 Issue: 4 Pages: 365-377
  • DOI: 10.1007/s12012-021-09718-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Long-term efficacy and safety of subcutaneous concizumab prophylaxis in hemophilia A and hemophilia A/B with inhibitors (2022)
  • Author(s): Shapiro Amy D.、Angchaisuksiri Pantep、Astermark Jan、Benson Gary、Castaman Giancarlo、Eichler Hermann、Jimenez-Yuste Victor、Kavakli Kaan、Matsushita Tadashi、Poulsen Lone Hvitfeldt、Wheeler Allison P.、Young Guy、Zupancic-Salek Silva、Oldenburg Johannes、Chowdary Pratima
  • Journal Title: Blood Advances Volume: - Issue: 11 Pages: 3422-3432
  • DOI: 10.1182/bloodadvances.2021006403
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development and validation of a novel qualitative test for plasma fibrinogen utilizing clot waveform analysis (2022)
  • Author(s): Suzuki Atsuo、Suzuki Nobuaki、Kanematsu Takeshi、Shinohara Sho、Kurono Hiroshi、Arai Nobuo、Okamoto Shuichi、Suzuki Naruko、Tamura Shogo、Kikuchi Ryosuke、Katsumi Akira、Kojima Tetsuhito、Matsushita Tadashi
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 434-434
  • DOI: 10.1038/s41598-021-04464-5
  • Peer Reviewed / Open Access
• [Journal Article] Notch1 haploinsufficiency in mice accelerates adipogenesis (2021)
  • Author(s): Yamaguchi Kazutoshi、Hayashi Motoharu、Uchida Yasuhiro、Cheng Xian Wu、Nakayama Takayuki、Matsushita Tadashi、Murohara Toyoaki、Takeshita Kyosuke
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 16761-16761
  • DOI: 10.1038/s41598-021-96017-z
  • Peer Reviewed / Open Access
• [Journal Article] Essential role of a carboxyl-terminal α-helix motif in the secretion of coagulation factor XI. (2021)
  • Author(s): Hayakawa Y, Tamura S, Suzuki N, Odaira K, Tokoro M, Kawashima F, Hayakawa F, Takagi A, Katsumi A, Suzuki A, Okamoto S, Kanematsu T, Matsushita T, Kojima T.
  • Journal Title: J Thromb Haemost. Volume: - Issue: 4 Pages: 920-930
  • DOI: 10.1111/jth.15242
  • Peer Reviewed / Open Access
• [Journal Article] Guidelines for the Management of von Willebrand Disease 2021 (2021)
  • Author(s): 日笠 聡, 渥美 達也, 石黒 精, 金子 誠, 高橋 芳右, 野上 恵嗣, 藤井 輝久, 堀内 久徳, 松井 太衛, 毛利 博, 森下 英理子, 松下 正, 朝比奈 俊彦, 天野 景裕, 上田 恭典, 岡本 好司, 小亀 浩市, 佐道 俊幸, 瀧 正志, 長尾 梓, 西尾 健治, 西田 恭治, 西野 正人, 藤村 吉博, 松本 雅則, 宮川 義隆, 八木 秀男, 和田 英夫
  • Journal Title: Japanese Journal of Thrombosis and Hemostasis Volume: 32 Issue: 4 Pages: 413-481
  • DOI: 10.2491/jjsth.32.413
  • NAID: 130008080765
  • ISSN: 0915-7441, 1880-8808
  • Peer Reviewed
• [Journal Article] The diagnosis and management of von Willebrand disease (2021)
  • Author(s): 松下 正
  • Journal Title: Rinsho Ketsueki Volume: 62 Issue: 8 Pages: 1205-1212
  • DOI: 10.11406/rinketsu.62.1205
  • NAID: 130008086027
  • ISSN: 0485-1439, 1882-0824
• [Journal Article] The management strategy for von Willebrand disease (2021)
  • Author(s): 松下 正
  • Journal Title: Rinsho Ketsueki Volume: 62 Issue: 5 Pages: 435-444
  • DOI: 10.11406/rinketsu.62.435
  • NAID: 130008049785
  • ISSN: 0485-1439, 1882-0824
• [Journal Article] Myh9 R702C is associated with erythroid abnormality with splenomegaly in mice (2021)
  • Author(s): Kanematsu T, Suzuki N, Tamura S, Suzuki A, Ishikawa Y, Katsumi A, Kiyoi H, Saito H, Kunishima S, Kojima T, Matsushita T.
  • Journal Title: Nagoya Journal of Medical Science Volume: 83 Issue: 1 Pages: 75-86
  • DOI: 10.18999/nagjms.83.1.75
  • NAID: 120007127313
  • ISSN: 0027-7622
  • URL: https://nagoya.repo.nii.ac.jp/records/2000205
  • Peer Reviewed / Open Access
• [Journal Article] Phase I clinical trial of intra-bone marrow cotransplantation of mesenchymal stem cells in cord blood transplantation. (2021)
  • Author(s): Goto T, Murata M, Nishida T, Terakura S, Kamoshita S, Ishikawa Y, Ushijima Y, Adachi Y, Suzuki S, Kato K, Hirakawa A, Nishiwaki S, Nishio N, Takahashi Y, Kodera Y, Matsushita T, Kiyoi H.
  • Journal Title: Stem Cells Transl Med. Volume: 10(4) Issue: 4 Pages: 542-553
  • DOI: 10.1002/sctm.20-0381
  • Peer Reviewed / Open Access
• [Journal Article] Safety and efficacy of percutaneous radiofrequency ablation for hepatocellular carcinoma patients with haemophilia. (2021)
  • Author(s): Yamamoto T, Imai N, Yamamoto K, Ito T, Ishizu Y, Honda T, Okamoto S, Kanematsu T, Suzuki N, Matsushita T, Ishigami M, Fujishiro M.
  • Journal Title: Haemophilia. Volume: 27(1) Issue: 1 Pages: 100-107
  • DOI: 10.1111/hae.14220
  • Peer Reviewed / Open Access
• [Journal Article] Long-term safety and efficacy of emicizumab for up to 5.8 years and patients' perceptions of symptoms and daily life: A phase 1/2 study in patients with severe haemophilia A (2021)
  • Author(s): Shima M, Nagao A, Taki M, Matsushita T, Oshida K, Amano K, Nagami S, Okada N, Maisawa S, Nogami K.
  • Journal Title: Haemophilia. Volume: 27(1) Issue: 1 Pages: 81-89
  • DOI: 10.1111/hae.14205
  • Peer Reviewed / Open Access
• [Journal Article] 特集 検査値を読む2020 4章 血栓･止血検査 von Willebrand因子切断酵素(ADAMTS13) (2020)
  • Author(s): 鈴木 伸明、松下 正
  • Journal Title: 内科 Volume: 125 Issue: 4 Pages: 561-562
  • DOI: 10.15106/j_naika125_561
  • ISSN: 0022-1961, 2432-9452
  • Year and Date: 2020-04-01
  • Int'l Joint Research
• [Journal Article] 特集 検査値を読む2020 4章 血栓･止血検査 von Willebrand因子(抗原,活性,マルチマー構造解析を含む) (2020)
  • Author(s): 鈴木 伸明、松下 正
  • Journal Title: 内科 Volume: 125 Issue: 4 Pages: 559-560
  • DOI: 10.15106/j_naika125_559
  • ISSN: 0022-1961, 2432-9452
  • Year and Date: 2020-04-01
  • Int'l Joint Research
• [Journal Article] 特集 検査値を読む2020 4章 血栓･止血検査 第XIII因子 (2020)
  • Author(s): 鈴木 伸明、松下 正
  • Journal Title: 内科 Volume: 125 Issue: 4 Pages: 557-558
  • DOI: 10.15106/j_naika125_557
  • ISSN: 0022-1961, 2432-9452
  • Year and Date: 2020-04-01
  • Int'l Joint Research
• [Journal Article] 特集 検査値を読む2020 4章 血栓･止血検査 第XI因子 (2020)
  • Author(s): 鈴木 伸明、松下 正
  • Journal Title: 内科 Volume: 125 Issue: 4 Pages: 555-555
  • DOI: 10.15106/j_naika125_555
  • ISSN: 0022-1961, 2432-9452
  • Year and Date: 2020-04-01
  • Int'l Joint Research
• [Journal Article] 特集 検査値を読む2020 4章 血栓･止血検査 第Ⅹ因子 (2020)
  • Author(s): 鈴木 伸明、松下 正
  • Journal Title: 内科 Volume: 125 Issue: 4 Pages: 553-554
  • DOI: 10.15106/j_naika125_553
  • ISSN: 0022-1961, 2432-9452
  • Year and Date: 2020-04-01
  • Int'l Joint Research
• [Journal Article] Vascular endothelial growth factor (VEGF)-A and VEGF-A165b are associated with time to remission of granulomatosis with polyangiitis in a nationwide Japanese prospective cohort study (2020)
  • Author(s): Kikuchi R、Tsuboi N、Sada K、Nakatochi M、Yokoe Y、Suzuki A、Maruyama S、Murohara T、Matsushita T、Amano K、Atsumi T、Takasaki Y、Ito S、Hasegawa H、Dobashi H、Ito T、Makino H、Matsuo S、Research Committee of Intractable Vasculitis Syndrome and Research Committee of Intractable
  • Journal Title: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine Volume: 58 Issue: 2 Pages: 86-94
  • DOI: 10.1177/0004563220968371
  • Peer Reviewed / Open Access
• [Journal Article] Impact of variation in reagent combinations for one‐stage clotting assay on assay discrepancy in nonsevere haemophilia A (2020)
  • Author(s): Suzuki Atsuo、Suzuki Nobuaki、Kanematsu Takeshi、Okamoto Shuichi、Tamura Shogo、Kikuchi Ryosuke、Katsumi Akira、Kiyoi Hitoshi、Kojima Tetsuhito、Matsushita Tadashi
  • Journal Title: International Journal of Laboratory Hematology Volume: 43 Issue: 1 Pages: 131-138
  • DOI: 10.1111/ijlh.13335
  • Peer Reviewed / Open Access
• [Journal Article] An overview of the pathfinder clinical trials program: Long‐term efficacy and safety of N8‐GP in patients with hemophilia A (2020)
  • Author(s): Matsushita Tadashi、Mangles Sarah
  • Journal Title: Journal of Thrombosis and Haemostasis Volume: Supp;1(Suppl1) Pages: 26-33
  • DOI: 10.1111/jth.14958
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Indoxyl Sulfate-induced Vascular Calcification is mediated through Altered Notch Signaling Pathway in Vascular Smooth Muscle Cells (2020)
  • Author(s): Yamaguchi Kazutoshi、Yisireyili Maimaiti、Goto Sumie、Kato Katsuhiro、Cheng Xian Wu、Nakayama Takayuki、Matsushita Tadashi、Niwa Toshimitsu、Murohara Toyoaki、Takeshita Kyosuke
  • Journal Title: International Journal of Medical Sciences Volume: 17 Issue: 17 Pages: 2703-2717
  • DOI: 10.7150/ijms.43184
  • Peer Reviewed / Open Access
• [Journal Article] A Japanese multi-institutional collaborative study of antigen-positive red blood cell (RBC) transfusions in patients with corresponding RBC antibodies. (2020)
  • Author(s): Yamada C, Takeshita A, Ohto H, Ishimaru K, Kawabata K, Nomaguchi Y, Haraguchi Y, Abe M, Sobue K, Takenouchi H, Takadate J, Kamimura M, Katai A, Kasai D, Minami Y, Sugimoto T, Michino J, Nagai K, Kumagai M, Hasegawa Y, Ishizuka K, Ohtomo N, Yamada N, Muroi K, Matsushita T, Takahashi K.
  • Journal Title: Vox Sang. Volume: - Issue: 5 Pages: 456-465
  • DOI: 10.1111/vox.12906
  • Peer Reviewed / Open Access
• [Journal Article] Aberrant X chromosomal rearrangement through multi‐step template switching during sister chromatid formation in a patient with severe hemophilia A (2020)
  • Author(s): Tokoro Mahiru、Tamura Shogo、Suzuki Nobuaki、Kakihara Misaki、Hattori Yuna、Odaira Koya、Suzuki Sachiko、Takagi Akira、Katsumi Akira、Hayakawa Fumihiko、Okamoto Shuichi、Suzuki Atsuo、Kanematsu Takeshi、Matsushita Tadashi、Kojima Tetsuhito
  • Journal Title: Molecular Genetics & Genomic Medicine Volume: 8 Issue: 9 Pages: 1-12
  • DOI: 10.1002/mgg3.1390
  • Peer Reviewed / Open Access
• [Journal Article] A survey of blood transfusion errors in Aichi Prefecture in Japan: Identifying major lapses threatening the safety of transfusion recipients (2020)
  • Author(s): Ri Masaki、Kasai Masanobu、Kohno Akio、Kondo Masaru、Sawa Masashi、Kinoshita Tomohiro、Sugiura Isamu、Miura Yasuo、Yamamoto Kazuhito、Saito Toshiki I.、Ozawa Yukiyasu、Matsushita Tadashi、Kato Hidefumi
  • Journal Title: Transfusion and Apheresis Science Volume: 59 Issue: 3 Pages: 102735-102735
  • DOI: 10.1016/j.transci.2020.102735
  • Peer Reviewed / Open Access
• [Journal Article] Long‐term safety and sustained efficacy for up to 5 years of treatment with recombinant factor IX Fc fusion protein in subjects with haemophilia B: Results from the B‐YOND extension study (2020)
  • Author(s): Pasi K. John、Fischer Kathelijn、Ragni Margaret、Kulkarni Roshni、Ozelo Margareth C.、Mahlangu Johnny、Shapiro Amy、P’Ng Stephanie、Chambost Herv?、Nolan Beatrice、Bennett Carolyn、Matsushita Tadashi、Winding Bent、Fruebis Joachim、Yuan Huixing、Rudin Dan、Oldenburg Johannes
  • Journal Title: Haemophilia Volume: 26 Issue: 6
  • DOI: 10.1111/hae.14036
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Higher FVIII:C Measured by Chromogenic Substrate Assay Than by One-Stage Assay Is Associated With Silent Hemophilic Arthropathy (2020)
  • Author(s): Mika Ogawa, Nobuaki Suzuki, Nobunori Takahashi, Shogo Tamura, Atsuo Suzuki, Sachiko Suzuki, Yuua Hattori, Misaki Kakihara, Takeshi Kanematsu, Toshihisa Kojima, Akira Katsumi, Fumihiko Hayakawa, Tetsuhito Kojima, Naoki Ishiguro , Hitoshi Kiyoi , Tadashi Matsushita
  • Journal Title: Thromb Res Volume: 188 Pages: 103-105
  • DOI: 10.1016/j.thromres.2020.01.003
  • NAID: 120006872431
  • Peer Reviewed / Open Access
• [Journal Article] Performance Evaluation of INNOVANCE^? VWF Ac Assay employing recombinant glycoprotein Ib mutant (2020)
  • Author(s): Suzuki Atsuo、Suzuki Nobuaki、Kanematsu Takeshi、Okamoto Shuichi、Tamura Shogo、Shinohara Sho、Arai Nobuo、Kikuchi Ryosuke、Ando Yoshitaka、Kojima Tetsuhito、Matsushita Tadashi
  • Journal Title: Japanese Journal of Thrombosis and Hemostasis Volume: 31 Issue: 4 Pages: 409-419
  • DOI: 10.2491/jjsth.31.409
  • NAID: 130007887027
  • Peer Reviewed
• [Presentation] 出血性疾患の新しい治療 (2022)
  • Author(s): 松下 正
  • Organizer: 第23回検査血液学会
  • Invited
• [Presentation] Multimerの経時的変化が止血効果の指標となり得た後天性von Willebrand症候群の1例 (2022)
  • Author(s): 岡本 修一, 鈴木 伸明, 兼松 毅, 鈴木 奈瑠子, 鈴木 敦夫, 清井 仁, 松下 正
  • Organizer: 第44回日本血栓止血学会学術集会
• [Presentation] Endothelial colony forming cells(ECFCs)の安定した樹立を目指して (2022)
  • Author(s): 岡本 修一, 鈴木 伸明, 田村 彰吾, 鈴木 敦夫, 大平 晃也, 兼松 毅, 鈴木 奈瑠子, 清井 仁, 松下 正
  • Organizer: 第70回日本輸血・細胞治療学会学術総会
• [Presentation] 出血性疾患に対する新しい治療 (2022)
  • Author(s): 松下 正
  • Organizer: 第70回日本輸血・細胞治療学会学術総会
  • Invited
• [Presentation] 出血性疾患への適切なアプローチ (2022)
  • Author(s): 松下 正
  • Organizer: 第67回内科学会北海道支部生涯教育講演会
  • Invited
• [Presentation] フィブリノゲンの質的異常を検出可能な新しい自動分析技術 凝固波形解析に基づくフィブリノゲン定性分析法のバリデーション (2021)
  • Author(s): 鈴木 敦夫, 篠原 翔, 新井 信夫, 安藤 善孝, 松下 正
  • Organizer: 第70回日本医学検査学会
• [Presentation] マウス血管結紮モデルによるアンチトロンビンレジスタンスの血栓形成解析 (2021)
  • Author(s): 鈴木 伸明，鈴木 敦夫，田村 彰吾，向出 将人，岡本 修一，兼松 毅，小嶋 哲人， 松下 正
  • Organizer: 第４３回日本血栓止血学会学術集会
• [Presentation] フィブリノゲン強制発現系の最適化に向けた基礎検討 (2021)
  • Author(s): 向出 将人，田村 彰吾，鈴木 伸明，大平 晃也，早川 友梨，岡田健太郎，藤井 渉， 桂木 裕実，鈴木 敦夫，兼松 毅，岡本 修一，松下 正，小嶋 哲人, 早川 文彦
  • Organizer: 第４３回日本血栓止血学会学術集会
• [Presentation] F8 Int22h-1 が関与するF8 大規模欠失挿入変異による重症血友病A 症例 (2021)
  • Author(s): 大平 晃也，早川 友梨，田村 彰吾，鈴木 伸明，所 真昼，河島 史華，向出 将人，岡田健太郎，桂木 裕実，藤井 渉，岡本 修一，兼松 毅，鈴木 敦夫，松下 正，小嶋 哲人，早川 文彦
  • Organizer: 第４３回日本血栓止血学会学術集会
• [Presentation] VWF G2752S 変異体の分子病態解析 (2021)
  • Author(s): 岡本 修一，大平 晃也，早川 友梨，河島 史華，田村 彰吾，鈴木 伸明，兼松 毅，鈴木 敦夫，早川 文彦，清井 仁，小嶋 哲人，松下 正
  • Organizer: 第４３回日本血栓止血学会学術集会
• [Presentation] p.G2752S Severely Affects Dimerization of von Willebrand Factor: An Analysis of Type 3 von Willebrand Disease (2021)
  • Author(s): S. Okamoto, S. Tamura, N. Suzuki, K. Odaira, Y. Hayakawa, A. Suzuki, T. Kanematsu, F. Hayakawa, H. Kiyoi, T. Kojima, T. Matsushita
  • Organizer: ISTH 2021
  • Int'l Joint Research
• [Presentation] A Critical Role of Carboxyl-Terminal Alpha-Helix in the Secretion of Coagulation Factor XI (2021)
  • Author(s): Y. Hayakawa, S. Tamura, N. Suzuki, K. Odaira, M. Tokoro, F. Kawashima, F. Hayakawa, A. Takagi, A. Suzuki, S. Okamoto, T. Kanemats3, T. Matsushita, T. Kojima
  • Organizer: ISTH 2021
  • Int'l Joint Research
• [Presentation] Impact of Combination of Factor VIII-Deficient Plasma and Activator in One-Stage Clotting Assay on Potency Testing of Factor VIII Products (2021)
  • Author(s): A. Suzuki, N. Suzuki, T. Kanematsu, S. Okamoto, S. Tamura, T. Kojima, T. Matsushita
  • Organizer: ISTH 2021
  • Int'l Joint Research
• [Presentation] Long-Term Safety and Efficacy of Emicizumab for up to >5 Years in a Phase 1/2 Study in Patients with Severe Hemophilia A (2021)
  • Author(s): M. Shima, A. Nagao, M. Taki, T. Matsushita, K. Oshida, K. Amano, S. Nagami, N. Okada, K. Nogami
  • Organizer: ISTH 2021
  • Int'l Joint Research
• [Presentation] The Haemophilia A Patients whose FVIII: C by Chromogenic Substrate Assay were Higher than by One-Stage Substrate Assay Showed Risk of Development of Haemophiliac Arthropathy Silently (2021)
  • Author(s): N. Suzuki, N. Takahashi, A. Suzuk2, S. Tamura, S. Suzuki, Y. Hattori, M. Kakihara, M. Ogawa, T. Kanematsu, T. Kojima, T. Kojima, H. Kiyoi, N. Ishiguro, T. Matsushita
  • Organizer: ISTH 2021
  • Int'l Joint Research
• [Presentation] Investigation of a Significant Factor to Improve an Establishment of Endothelial Colony Forming Cell (2021)
  • Author(s): S. Okamoto, N. Suzuki, T. Kanematsu, A. Suzuki, K. Odaira, M. Tokoro, Y. Hayakawa, S. Tamura, F. Hayakaw5, H. Kiyoi, T. Kojima, T. Matsushita
  • Organizer: ISTH 2021
  • Int'l Joint Research
• [Presentation] 1009 The E6cacy and Safety of Caplacizumab in Japanese Patients with Immune-Mediated Thrombotic Thrombocytopenic Purpura (iTTP): An Open-Label, Phase 2/3 Study (2021)
  • Author(s): Yoshitaka Miyakawa , Kazunori Imada, Satoshi Ichikawa, Hitoji Uchiyama, Yasunori Ueda, Akihito Yonezawa, Shigeki Fujitani, Hiroshi Handa, Tadashi Matsushita, Hidesaku Asakura, Kenji Nishio, Kodai Suzuki, Yasuhiro Hashimoto , Shinobu Ohshima , Sayaka Tahara , Tomoyuki Tanaka, and Masanori Matsumoto,
  • Organizer: ASH2021
  • Int'l Joint Research
• [Presentation] Endothelial colony forming cells(ECFCs)樹立法の標準化への試み (2020)
  • Author(s): 岡本 修一, 鈴木 伸明, 兼松 毅, 鈴木 敦夫, 大平 晃也, 所 真昼, 早川 友梨, 田村 彰吾, 早川 文彦, 清井 仁, 小嶋 哲人, 松下 正
  • Organizer: 第４２回日本血栓止血学会学術集会
• [Presentation] 高齢者における静脈血栓塞栓症発症の症例対照研究 (2020)
  • Author(s): 勝見 章, 室谷 健太, 鴨下 園子, 伊藤 美由紀, 田村 彰吾, 鈴木 伸明, 松下 正, 小嶋 哲人
  • Organizer: 第４２回日本血栓止血学会学術集会
• [Presentation] Template switchingによるF8エクソン15欠損が認められた重症血友病A症例 (2020)
  • Author(s): 所 真昼, 田村 彰吾, 鈴木 伸明, 大平 晃也, 河島 史華, 早川 友梨, 早川 文彦, 岡本 修一, 兼松 毅, 勝見 章, 松下 正, 小嶋 哲人
  • Organizer: 第４２回日本血栓止血学会学術集会
• [Presentation] 特発性血栓症患者のゲノム情報を用いた層別化による病態解明研究 (2020)
  • Author(s): 辻 明宏, 和田 英夫, 森下 英理子, 野上 恵嗣, 荻原 義人, 松下 正, 小嶋 哲人, 大郷 剛, 根木 玲子, 小亀 浩市, 安田 聡, 山田 典一, 関根 章博, 宮田 敏行
  • Organizer: 第４２回日本血栓止血学会学術集会
• [Presentation] 凝固一段法による第VIII因子活性測定における試薬の組み合わせに関する検討 (2020)
  • Author(s): 鈴木 敦夫, 鈴木 伸明, 兼松 毅, 岡本 修一, 田村 彰吾, 安藤 善孝, 清井 仁, 松下 正
  • Organizer: 第４２回日本血栓止血学会学術集会
• [Presentation] C末端伸長変異型Factor XIにおける分泌異常の解析 (2020)
  • Author(s): 早川 友梨, 田村 彰吾, 鈴木 伸明, 大平 晃也, 所 真昼, 河島 史華, 早川 文彦, 高木 明, 鈴木 敦夫, 岡本 修一, 兼松 毅, 松下 正, 小嶋 哲人
  • Organizer: 第４２回日本血栓止血学会学術集会
• [Presentation] スプライシング異常を引き起こす血液凝固第IX因子遺伝子イントロン1内の深部大挿入変異 (2020)
  • Author(s): 河島 史華, 田村 彰吾, 鈴木 伸明, 大平 晃也, 所 真昼, 早川 友梨, 早川 文彦, 岡本 修一, 兼松 毅, 高木 明, 松下 正, 小嶋 哲人
  • Organizer: 第４２回日本血栓止血学会学術集会
• [Presentation] Real-World Effectiveness Evaluation of the Recombinant Factor VIII Fc Fusion Protein in Adolescents and Children with Hemophilia A in Japan: "Fc Adolescent and Children Treatment" Study (FACTs) Part 1 Interim Analysis (2020)
  • Author(s): M. Kobayashi, T. Matsushita, K. Nogami, I. Usami, M. Shiraishi, M. Takatok
  • Organizer: ISTH 2020 Congress
  • Int'l Joint Research
• [Presentation] Low-factor consumption and cost effectiveness for surgeries in hemophilia B patients with N9-GP (2020)
  • Author(s): 岡本修一 松下正 兼松毅 鈴木敦夫 田村彰吾 小嶋哲人 鈴木伸明
  • Organizer: ISTH 2020 Congress
  • Int'l Joint Research
• [Presentation] Investigation of a significant factor to improve an establishment of endothelial colony forming cell (2020)
  • Author(s): 岡本修一 鈴木伸明 兼松毅 鈴木敦夫 大平晃也 所真昼 早川友梨 田村彰吾 早川文彦 清井仁 小嶋哲人 松下正
  • Organizer: ISTH 2020 Congress
  • Int'l Joint Research
• [Presentation] Molecular pathology of VWF G2752S mutation identified in a VWD patient with severe VWF defect (2020)
  • Author(s): Shuichi Okamoto ，Koya Odaira ，Yuri Hayakawa ，Fumika Kawashima ，Shogo Tamura ，Nobuaki Suzuki ，Takeshi Kanematsu ，Atsuo Suzuki ，Fumihiko Hayakawa ,Hitoshi Kiyoi ，Tetsuhito Kojima ，Tadashi Matsushita
  • Organizer: 第82回血液学会学術集会
• [Presentation] A critical role of carboxyl-terminal α-helix in secretion of coagulation factor XI (2020)
  • Author(s): Yuri Hayakawa ，Shogo Tamura ，Nobuaki Suzuki ，Koya Odaira ，Mahiru Tokoro ，Fumika Kawashima ，Fumihiko Hayakawa ，Atsuo Suzuki ，Shuichi Okamoto ，Takeshi Kanematsu ，Akira Katsumi ，Tadashi Matsushita ，Tetsuhito Kojima
  • Organizer: 第82回血液学会学術集会
• [Presentation] フォンヴィレブランド病の病態と治療、臨床試験データについて (2020)
  • Author(s): Tadashi Matsushita
  • Organizer: Takeda von Willebrand disease online in June(武田薬品工業)web
  • Invited
• [Presentation] von Wellebrand因子とvon Willebrand病 (2020)
  • Author(s): Tadashi Matsushita
  • Organizer: Takeda von Wellebrand disease symposium
  • Invited
• [Presentation] von Wellebrand因子とvon Willebrand病 (2020)
  • Author(s): Tadashi Matsushita
  • Organizer: 第16回埼玉血友病地域連携ネットワーク・オンライン
  • Invited
• [Patent(Industrial Property Rights)] 血液検体の分析方法、分析装置及びコンピュータプログラム (2020)
  • Inventor(s): 鈴木敦夫、松下正、篠原翔、新井信夫
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020
  • Acquisition Date: 2020
  • Overseas