Elucidation of genotype-phenotype relation in pituitary tumor through novel proteomics analysis
Project/Area Number |
20K08880
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 54040:Metabolism and endocrinology-related
|
Research Institution | Chiba University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
田中 知明 千葉大学, 大学院医学研究院, 教授 (50447299)
|
Project Period (FY) |
2020-04-01 – 2023-03-31
|
Project Status |
Completed (Fiscal Year 2022)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 下垂体腺腫 / 遺伝子型―表現型連関 / プロテオミクス / マルチオミクス / 遺伝子型―表現型 連関 / 下垂体腫瘍 / 遺伝子 / ゲノム |
Outline of Research at the Start |
本研究では、遺伝子変異解析(NGS/TCS解析)情報に、新しい網羅的タンパク発現定量解析・臨床病理学的特徴のスコア化を統合することで、複雑かつ多様な病態をより個別化して捉え、手術困難や治療効果を予測できるような基盤的研究を推進する。その結果、機能性下垂体腫瘍の薬剤治療反応性や手術リスク・寛解予測を目指した革新的個別化医療の創出が期待できる。
|
Outline of Final Research Achievements |
We performed RNA-sequencing and non-targeted proteomics using pituitary adenoma tumor samples. mRNA and protein expression in the tumor tissue showed a positive correlation, and no differences were observed between tumors. Analysis using UMAP confirmed that both RNA-sequencing and protein expression were divided into well-known lineages, suggesting that the molecular and cellular biology basis of metabolic and biological functions in the tumor tissue of pituitary adenomas may affect the correlation between mRNA and protein expression. However, it was also found that there are limitations to multi-omics data analysis using "bulk samples" of the entire tumor tissue.
|
Academic Significance and Societal Importance of the Research Achievements |
下垂体腺腫における遺伝子型―表現型連関の解明を目的とした本研究においては、新たな技術である網羅的タンパク定量解析(non-target proteomics)およびRNA-sequencingにおけるMulti-omics data解析を用いることで、機能性と腫瘍性の次元軸から表出される多彩で複雑な下垂体腺腫の臨床像を説明し得る分子細胞生物学的交絡因子の一端を探索することができた。本研究によって得られた知見及び既存の遺伝子変異解析情報を有効に活用することにより、下垂体腫瘍の分子生物学的背景を解明することで,薬剤治療反応性や手術リスク及び寛解予測を目指した革新的個別化医療の創出をが期待される。
|
Report
(4 results)
Research Products
(41 results)
-
-
-
[Journal Article] Proteogenomic landscape and clinical characterization of GH-producing pituitary adenomas/somatotroph pituitary neuroendocrine tumors2022
Author(s)
Yamato A,Nagano H,Gao Y,Matsuda T,Hashimoto N,Nakayama A,Yamagata K,Yokoyama M,Gong Y,Shi X,Zhahara N,Kono T,Taki Y,Furuki N,Nishimura M,Horiguchi K,Iwadate Y,Fukuyo M,Rahmutulla B,Kaneda A,Hasegawa Y,Kawashima Y,Ohara O,Ishikawa T,Kawakami E,Nakamura Y,Inoshita N,Yamada S,Fukuhara N,Nishioka H,Tanaka T
-
Journal Title
Communications Biology
Volume: 5
Issue: 1
Pages: 1304-1304
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
[Journal Article] Multi-Omicsから見た下垂体神経内分泌腫瘍の特性2021
Author(s)
高躍,村田和貴, 大和梓, 永野秀和, 松田達磨, 堀口健太郎, 岩立康男, 福原紀章, 西岡宏, 山田正三, 井下尚子, 田中知明
-
Journal Title
Folia Endocrinologica Japonica
Volume: 97
Issue: S.HPT
Pages: 104-106
DOI
NAID
ISSN
0029-0661, 2186-506X
Year and Date
2021-09-20
Related Report
Open Access
-
-
[Journal Article] 手術アプローチに工夫を要した下垂体癌の一例2021
Author(s)
松田達磨,堀口健太郎,高躍,岩立康男
-
Journal Title
Folia Endocrinologica Japonica
Volume: 97
Issue: S.HPT
Pages: 84-86
DOI
NAID
ISSN
0029-0661, 2186-506X
Year and Date
2021-09-20
Related Report
Open Access
-
[Journal Article] Two cases of symptomatic secondary hypophysitis due to Rathke’s cleft cysts treated with glucocorticoids: long-term follow-up2021
Author(s)
Hanna Deguchi-Horiuchi, Hisashi Koide, Ikki Sakuma, Yue Gao, Seiichiro Higuchi, Hidekazu Nagano, Naoko Hashimoto, Kentaro Horiguchi, Yasuo Iwadate, Naoko Inoshita, Koutaro Yokote, Tomoaki Tanaka
-
Journal Title
Endocrine Journal
Volume: 68
Issue: 3
Pages: 269-279
DOI
NAID
ISSN
0918-8959, 1348-4540
Related Report
Peer Reviewed / Open Access
-
[Journal Article] ICAM1-Negative Intravascular Large B-Cell Lymphoma of the Pituitary Gland: A Case Report and Literature Review2021
Author(s)
Kumiko Nait, Sawako Suzuki, Chikako Ohwada, Kazuki Ishiwata, Yutaro Ruike, Akiko Ishida, Hanna Deguchi, Masanori Fujimoto, Hisashi Koide, Emiko Sakaida, Kentaro Horiguchi, Yasuo Iwadate, Ichiro Tatsuno, Naoko Inoshita, Jun-ichiro Ikeda, Tomoaki Tanaka, Koutaro Yokote,
-
Journal Title
AACE Clinical Case Reports
Volume: -
Issue: 4
Pages: 249-255
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Multi-Omics から見た下垂体神経内分泌腫瘍の特性2021
Author(s)
高躍,村田和貴, 大和梓, 永野秀和, 松田達磨, 堀口健太郎, 岩立康男, 福原紀章, 西岡宏, 山田正三, 井下尚子, 田中知明
Organizer
第31回日本間脳下垂体腫瘍学会
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-