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Study of the role of Paxillin, focal adhesion associated factor, in the early stage of colorectal cancer to liver metastasis

Research Project

Project/Area Number 20K09067
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 55020:Digestive surgery-related
Research InstitutionOsaka Metropolitan University (2022)
Osaka City University (2020-2021)

Principal Investigator

Fujioka Masaki  大阪公立大学, 大学院医学研究科, 特任講師 (10648463)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords腫瘍肝転移 / 接着斑 / 細胞基質接着装置 / 肝転移モデルマウス / 腫瘍転移 / 肝転移 / 細胞接着斑 / 生体イメージング / 大腸癌肝転移 / in vivoイメージング / Focal adhesion / Paxillin
Outline of Research at the Start

これまでに大腸がん肝転移患者検体を用いたNGS解析において、Focal adhesion(FA)関連遺伝子群の有意な発現上昇および予後との関連が複数報告されており、FAが転移成立に重要な役割を果たすことが示唆されている。しかし、FAはガラスやプラスチックのような硬い基質でみられる表現型であり、生体のような柔らかい環境下では観察されないという批判がある。したがって、生体内においてFAが存在するのか、また転移巣成立との関連について明らかにする必要がある。本研究では生体イメージング手法を用いて、転移におけるFAの有無およびその接着基質が何か明らかにし、さらに分子的機能について明らかにする。

Outline of Final Research Achievements

Several reports have shown significant upregulation of Focal adhesion (FA)-related genes and their association with prognosis in NGS analysis of liver metastatic tumor samples from cancer patients. This study focused on the involvement of cell-substrate adhesion proteins in tumor liver metastasis.
In the liver metastasis model via the splenic vein, a significant increase in Paxillin expression was observed in the parental strain and the liver metastasis-forming strain, and a higher expression of Paxillin was confirmed in the strain with enhanced liver metastatic potential by in vivo selection method. On the other hand, no significant change in Paxillin expression was observed in the lung metastases induced by intravesical injection compared to the parental strain.
These results suggest that Paxillin expression plays an essential role in liver and lung metastasis to bladder carcinoma, but the mechanism of its establishment in the metastases may differ.

Academic Significance and Societal Importance of the Research Achievements

これまでにがん肝転移患者検体を用いたNGS解析において、Focal adhesion(FA)関連遺伝子群の有意な発現上昇および予後との関連が複数報告されている。したがって、FAが肝転移巣成立に重要な役割を果たすことが示唆されている。
本研究の成果により、血流を介した腫瘍の肝転移巣形成過程において、Paxillinを含むFocal adhesion関連因子の発現が転移巣の形成に重要な役割を果たすことが示唆され、転移を抑制する為の新たな治療標的としての可能性を示すことができた。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (11 results)

All 2023 2022 2021 2020

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (4 results) (of which Int'l Joint Research: 2 results) Book (1 results)

  • [Journal Article] Irradiation plus myeloid-derived suppressor cell-targeted therapy for overcoming treatment resistance in immunologically cold urothelial carcinoma2023

    • Author(s)
      Yamamoto Shoma、Kato Minoru、Takeyama Yuji、Azuma Yukari、Yukimatsu Nao、Hirayama Yukiyoshi、Otoshi Taiyo、Yamasaki Takeshi、Fujioka Masaki、Gi Min、Wanibuchi Hideki、Uchida Junji
    • Journal Title

      British Journal of Cancer

      Volume: . Issue: 12 Pages: 1-9

    • DOI

      10.1038/s41416-023-02244-8

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] o-Toluidine metabolism and effects in the urinary bladder of humanized-liver mice2023

    • Author(s)
      Yokota Y, Suzuki S, Gi M, Yanagiba Y, Yoneda N, Fujioka M, Kakehashi A, Koda S, Suemizu H, Wanibuchi H
    • Journal Title

      Toxicology

      Volume: 488 Pages: 153483-153483

    • DOI

      10.1016/j.tox.2023.153483

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The carbonic anhydrase inhibitor acetazolamide inhibits urinary bladder cancers via suppression of β-catenin signaling.2022

    • Author(s)
      Matsue T, Gi M, Shiota M, Tachibana H, Suzuki S, Fujioka M, Kakehashi A, Yamamoto T, Kato M, Uchida J, Wanibuchi H.
    • Journal Title

      Cancer Sci.

      Volume: 113 Issue: 8 Pages: 2642-2653

    • DOI

      10.1111/cas.15467

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cache Domain Containing 1 Is a Novel Marker of Non-Alcoholic Steatohepatitis-Associated Hepatocarcinogenesis2021

    • Author(s)
      Kakehashi Anna、Chariyakornkul Arpamas、Suzuki Shugo、Khuanphram Napaporn、Tatsumi Kumiko、Yamano Shotaro、Fujioka Masaki、Gi Min、Wongpoomchai Rawiwan、Wanibuchi Hideki
    • Journal Title

      Cancers

      Volume: 13 Issue: 6 Pages: 1216-1216

    • DOI

      10.3390/cancers13061216

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Accumulation of 8-hydroxydeoxyguanosine, L-arginine and Glucose Metabolites by Liver Tumor Cells Are the Important Characteristic Features of Metabolic Syndrome and Non-Alcoholic Steatohepatitis-Associated Hepatocarcinogenesis2020

    • Author(s)
      Kakehashi Anna、Suzuki Shugo、Ishii Naomi、Okuno Takahiro、Kuwae Yuko、Fujioka Masaki、Gi Min、Stefanov Vasily、Wanibuchi Hideki
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 20 Pages: 7746-7746

    • DOI

      10.3390/ijms21207746

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dimethylarsinic acid (DMA) enhanced lung carcinogenesis via histone H3K9 modification in a transplacental mouse model2020

    • Author(s)
      Fujioka, M., Suzuki, S., Gi, M., Kakehashi, A., Oishi, Y., Okuno, T., Yukimatsu, N., Wanibuchi, H.
    • Journal Title

      Arch Toxicol

      Volume: 94 Issue: 3 Pages: 927-937

    • DOI

      10.1007/s00204-020-02665-x

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] マウス経胎盤曝露モデルにおけるジフェニルアルシン酸(DPAA)のエピジェネティック修飾異常を介した肝発がんの亢進2023

    • Author(s)
      藤岡正喜,魏民,鈴木周五、芝野佳奈、邱桂鈺、Vachiraarunwong Arpamas,Aroonrat Pharapirom,大石裕司、梯アンナ,鰐渕英機
    • Organizer
      第39回日本毒性病理学会学術集会及び総会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Diphenylarsinic acid induced hepatocarcinogenesis via altered DNA methylation in a transplacental mouse model2022

    • Author(s)
      Masaki Fujioka, Min Gi, Shugo Suzuki, Yuji Oishi, Vachiraarunwong Arpamas, Guiyu Qiu, Kana Shibano, Anna Kakehashi, Hideki Wanibuchi
    • Organizer
      第81回日本癌学会学術総会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 無機ヒ素曝露ヒト肝マウスにおける 尿中及び糞中ヒ素の化学形態別性状の解析2022

    • Author(s)
      藤岡正喜,魏民,Vachiraarunwon Arpamas,鈴木周五,鰐渕英機
    • Organizer
      第27回ヒ素シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] 有機ヒ素化合物ジメチルアルシン酸(DMA)はマウス経胎盤ばく露によりヒストンH3K9修飾を介した肺発がんを促進する2020

    • Author(s)
      藤岡正喜、鈴木周五、魏民、梯アンナ、大石祐司、鰐渕英機
    • Organizer
      2020年度文部科学省新学術領域研究 先端モデル動物支援プラットフォーム 若手支援技術講習会
    • Related Report
      2020 Research-status Report
  • [Book] マウス・ラットモデル作製・解析プロフェッショナル2021

    • Author(s)
      先端モデル動物支援プラットフォーム(AdAMS)
    • Total Pages
      320
    • Publisher
      羊土社
    • ISBN
      9784758121125
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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