| Project/Area Number |
20K09384
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 56010:Neurosurgery-related
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
Makoto Oishi 新潟大学, 脳研究所, 教授 (00422593)
|
|---|
| Project Period (FY) |
2020-04-01 – 2024-03-31
|
| Project Status |
Completed (Fiscal Year 2023)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | optical imaging / flavoprotein / brain function / monitoring |
|---|
| Outline of Research at the Start |
脳神経外科手術において，脳機能の局在とその広がりを把握したいという欲求は高く，今まで電気生理学的手法（モニタリング，マッピング，覚醒手術）や解剖学的検索（ナビゲーション）などが駆使されて来たが，神経活動と関連した機能野を術野に直接可視化できる方法は存在しなかった．我々は，動物実験を通して方法論は確立されていたフラビン蛍光法を，脳神経外科術中に大脳皮質表面の電気刺激による活動域を可視化することをすでに実現しており，本研究では，本方法をより現実的なてんかん焦点診断法，機能野同定法，術中モニタリング法として確立することを目指すものである．
|
| Outline of Final Research Achievements |
n the present study, we set out to detect neural activity using intraoperative flavoprotein fluorescence imaging (iFFI), which involves visualization of fluorescence changes derived from aerobic energy metabolism. In 7 brain tumor patients, neural activities were induced by direct electrical stimulation of surrounding cortex, and iFFI and intraoperative PDI (iPDI) were performed. Optical response of iFFI demonstrated a biphasic time course in which a short time increase in fluorescence was followed by a negative fluorescence change. By iPDI, only a relatively long-lasting negative signals were observed. The positive component of iFFI signals appeared the fastest and were most localized. These results suggest that detection of the positive component in iFFI enables earlier and more accurate detection of neural activity, potentially having profound influence on intraoperative detection of neural activity.
|
| Academic Significance and Societal Importance of the Research Achievements |
我々は脳表刺激によるヒト大脳皮質での賦活活動のフラビン蛍光法による可視化をはじめて成し遂げた．大脳皮質の活動の可視化は今後の脳機能の研究に大きな発展を及ぼす可能性があると考えている．現在，小さな大脳誘発電位の観察にトライしており，いまだ難しい点が多いが，あと一息のところにある．目先を変えれば，てんかん性自発放電などにも応用してゆき，脳神経外科手術の安全性に貢献できる手術支援法としての確立に努めたい．
|
