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Elucidation of the Mechanism of Diabetic Nephropathy Development Involving Periodontal Bacteria

Research Project

Project/Area Number 20K09909
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57020:Oral pathobiological science-related
Research InstitutionKagoshima University

Principal Investigator

Ohnishi Tomokazu  鹿児島大学, 医歯学域歯学系, 准教授 (30244247)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords歯周炎 / IgA腎症 / 腎糸球体 / IgA / Treponema / メサンギウム細胞 / 糖尿病 / TLRシグナル / Treponema Sp / 歯周病菌 / 扁桃 / 糖尿病性腎症 / メサンギウム領域 / COSMC
Outline of Research at the Start

糖尿病性腎症の多くはIgA腎症の可能性がある。近年、扁桃の切除が治療として有効であることから、細菌誘導説がIgA腎症の病因として有力になっており、とくに歯周病菌が原因菌の候補となっている。しかし、歯周病菌が誘導するIgA腎症を伴う糖尿病性腎症を解明するための動物モデルの報告はない。そこで本研究では、歯周病菌感染による歯周炎を糖尿病マウスに惹起することでIgA腎症を発症させその病態を探る。

Outline of Final Research Achievements

We established an experimental mouse model of periodontitis induced by Treponema infection to investigate the synergistic effect of periodontal bacterial infection and type 2 diabetes in IgA nephropathy. Wild-type mice were fed a high-fat diet for 4 months and then administered Treponema. As a result, we observed an increase in urinary albumin, IgG, and IgA levels, expansion of the mesangial area, and IgA deposition. To investigate the signaling molecule Tpl2 as a mediator of periodontal bacterial stimulation, we performed the same treatment on Tpl2 knockout mice and found no increase in urinary IgG or IgA levels, and no expansion of the mesangial area or IgA deposition. These findings suggest that Tpl2 plays an important role in the development of IgA nephropathy in the context of the synergistic effect of periodontal bacterial infection and type 2 diabetes.

Academic Significance and Societal Importance of the Research Achievements

IgA腎症の治療方法として扁桃摘出が確立されており、その摘出された扁桃の臨床研究から、Treponemaの扁桃への感染が予後に関わる因子として報告されている。また、糖尿病性腎症と言われている腎疾患の多くはIgA腎症に関わりが深いことが知られている。しかし、糖尿病モデルマウスから派生したIgA腎症モデルは報告がない。そこで、本研究では二型糖尿病発症マウスにTreponema を投与し、実験的歯周炎を誘導することでIgA腎症を発症するマウスモデルを確率的に作製した。さらに、このモデルを用いどのような遺伝子欠損がgA腎症発症に関わるかを示した。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (10 results)

All 2022 2021 2020

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (4 results)

  • [Journal Article] EGR plays an important role in BMP9‐mediated osteoblast differentiation by promoting SMAD 1/5 phosphorylation2022

    • Author(s)
      Chiba Norika、Noguchi Yukie、Seong Chang Hwan、Ohnishi Tomokazu、Matsuguchi Tetsuya
    • Journal Title

      FEBS Letters

      Volume: 596 Issue: 13 Pages: 1720-1732

    • DOI

      10.1002/1873-3468.14407

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Periodontitis promotes the expression of gingival transmembrane serine protease 2 (TMPRSS2), a priming protease for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)2022

    • Author(s)
      Ohnishi T, Nakamura T, Shima K, Noguchi K, Chiba N, Matsuguchi T.
    • Journal Title

      J Oral Bioscience

      Volume: 64 Issue: 2 Pages: 229-236

    • DOI

      10.1016/j.job.2022.04.004

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] HIF‐1α plays an essential role in BMP9‐mediated osteoblast differentiation through the induction of a glycolytic enzyme, PDK12022

    • Author(s)
      Amir Muhammad Subhan、Chiba Norika、Seong Chang Hwan、Kusuyama Joji、Eiraku Nahoko、Ohnishi Tomokazu、Nakamura Norifumi、Matsuguchi Tetsuya
    • Journal Title

      Journal of Cellular Physiology

      Volume: 237 Issue: 4 Pages: 2183-2197

    • DOI

      10.1002/jcp.30752

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultraviolet B irradiation decreases CXCL10 expression in keratinocytes through endoplasmic reticulum stress2021

    • Author(s)
      Tomokazu Ohnishi, Mitsuhiro Hisadome, Kusuyama Joji, Norika Chiba, Muhammad Subhan Amir, Takuro Kanekura, Tetsuya Matsuguchi
    • Journal Title

      J Cell Biochem

      Volume: 122 Issue: 9 Pages: 1141-1156

    • DOI

      10.1002/jcb.29936

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bone morphogenetic protein 9 (BMP9) directly induces Notch effector molecule Hes1 through the SMAD signaling pathway in osteoblasts2021

    • Author(s)
      Seong CH, Chiba N, Kusuyama J, Subhan Amir M, Eiraku N, Yamashita S, Ohnishi T, Nakamura N, Matsuguchi T.
    • Journal Title

      FEBS Lett.

      Volume: 595 Issue: 3 Pages: 389-403

    • DOI

      10.1002/1873-3468.14016

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Glut1 expression is increased by p53 reduction to switch metabolism to glycolysis during osteoblast differentiation2020

    • Author(s)
      Ohnishi Tomokazu、Kusuyama Joji、Bandow Kenjiro、Matsuguchi Tetsuya
    • Journal Title

      Biochemical Journal

      Volume: - Issue: 10 Pages: 1795-1811

    • DOI

      10.1042/bcj20190888

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 歯周炎によるSARS-CoV-2のプライミング因子であるTMPRSS2の歯肉発現増強2022

    • Author(s)
      大西智和、千葉紀香、松口徹也
    • Organizer
      歯科基礎医学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] UVB irradiation reduces CXCL10 expression in human keratinocytes via ER stress2021

    • Author(s)
      大西智和、久留光博、金蔵拓郎、松口徹也
    • Organizer
      第35回表皮細胞研究会
    • Related Report
      2021 Research-status Report
  • [Presentation] All-trans retinoic acid induces Hes1-mediated downregulation of Tnfrsf11b mRNA expression in osteoblasts2020

    • Author(s)
      大西智和、成昌奐、千葉紀香、松口徹也
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 歯周炎による歯肉上皮系細胞のTransmembrane serine protease 2(TMPRSS2)発現上昇2020

    • Author(s)
      大西智和、千葉紀香、松口徹也
    • Organizer
      第2回南九州歯学会総会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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