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Molecular Biological analysis of virulence factors possessed by animal-derived periodontal pathogen P. gulae

Research Project

Project/Area Number 20K09918
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57020:Oral pathobiological science-related
Research InstitutionOkayama University

Principal Investigator

Inaba Hiroaki  岡山大学, 医歯薬学域, 准教授 (70359850)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsP. gulae / 線毛 / プロテアーゼ / LPS / 病原因子 / 歯周病 / 歯周病菌 / プロテーゼ / 炎症反応
Outline of Research at the Start

動物由来歯周病菌 Porphyromonas gulae が保有する病原因子による宿主への影響について解析する。本研究課題では、① P. gulae 病原因子(線毛、LPS、プロテアーゼ)の機能を解析する。② カイコモデルを使用した P. gulae 病原因子の病原性を評価する。③ 炎症ならびに細胞死を制御するシグナル伝達機構を解明する。これらの内容を明らかにし、歯周病発症を詳細に理解し、その制御法の開拓につながる知見を得て、新たなる菌による感染症の予防対策への足がかりとなる

Outline of Final Research Achievements

This research fund found the functional analysis of P. gulae virulence factors, including fimbriae, proteases, and LPS. Fimbriae analysis: FimA-deficient strain and a complementary strain were used in this study. Fimbriae was involved in P. gulae adherence and invasion on human gingival epithelial cells, while their properties, such as bacterial proliferation, black pigmentation, were not. LPS analysis: P. gulae LPS modulate the activity of multiple signaling
pathways via TLR2 and TLR4, resulting in the increase in inflammatory responses. Protease analysis: P. gulae proteases have an impact on hemagglutination, coaggregation with A. viscosus, and are required for bacterial growth. In addition, cell contact and adhesion-related human proteins were degraded by P. gulae proteases. Moreover, P. gulae proteases were shown to cleave γ-globulin, fibrinogen, and fibronectin.

Academic Significance and Societal Importance of the Research Achievements

P. gulae 感染は人畜共通感染症の可能性を持ち、人類の歯周病発症に関与する可能性が知らせている。本研究課題により明らかになったP. gulae 病原因子群の機能解析を世界に先駆けて報告すると共に、歯周病発症との関連性を明らかにすることができ学術的意義は高いと考えらえる。これら明らかになった病原因子群の機能をターゲットにした予防方法、治療方法の開発、ならびにP. gulae感染症に対する創薬につなぐことが出来る一端を証明することができた。また、医療関係者や患者への教育内容を充実させることができ、社会的な意義も高いと考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (23 results)

All 2023 2022 2021 2020

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 7 results) Presentation (13 results)

  • [Journal Article] Possible association of fimA genotype of Porphyromonas gulae with the severity of periodontal disease and the number of permanent teeth in dogs2023

    • Author(s)
      Shirahata S, Iwashita N, Sasaki R, Nomura R, Murakami M, Yasuda J, Yasuda H, Nakajima K, Inaba H, Matsumoto-Nakano M, Nakano K, Uchiyama J, Fukuyama T.
    • Journal Title

      Frontiers in Veterinary Science

      Volume: 10 Pages: 1022838-1022838

    • DOI

      10.3389/fvets.2023.1022838

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Green tea catechins inhibit Porphyromonas gulae LPS-induced inflammatory responses in human gingival epithelial cells2022

    • Author(s)
      Yoshida Sho、Inaba Hiroaki、Nomura Ryota、Nakano Kazuhiko、Matsumoto-Nakano Michiyo
    • Journal Title

      Journal of Oral Biosciences

      Volume: 64 Issue: 3 Pages: 352-358

    • DOI

      10.1016/j.job.2022.05.006

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of protease from animal periodontal pathogen Porphyromonas gulae2022

    • Author(s)
      Urmi SA, Inaba H, Yoshida S,Nomura R, Nakano K, Matsumoto-Nakano M.
    • Journal Title

      Japanese Journal of Veterinary Research

      Volume: 70 Pages: 79-90

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prognostic association of starvation-induced gene expression in head and neck cancer2021

    • Author(s)
      Hamada Masakazu、Inaba Hiroaki、Nishiyama Kyoko、Yoshida Sho、Yura Yoshiaki、Matsumoto-Nakano Michiyo、Uzawa Narikazu
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 1-16

    • DOI

      10.1038/s41598-021-98544-1

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficacy of FimA antibody and clindamycin in silkworm larvae stimulated with Porphyromonas gulae.2021

    • Author(s)
      Yoshida S, Inaba H, Nomura R, Murakami M, Yasuda H, Nakano K, Matsumoto-Nakano M
    • Journal Title

      Journal of Oral Microbiology

      Volume: 13 Issue: 1 Pages: 1914499-1914499

    • DOI

      10.1080/20002297.2021.1914499

    • NAID

      120007037383

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Roles of Porphyromonas gulae proteases in bacterial and host cell biology2021

    • Author(s)
      Urmi AS, Inaba H, Nomura R, Yoshida S, Ohara N, Asai F, Nakano K, Matsumoto-Nakano M
    • Journal Title

      Cellular Microbiology

      Volume: in press Issue: 8

    • DOI

      10.1111/cmi.13312

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Altered virulence of fimA variant strains in a silkworm infection model of Porphyromonas gulae2021

    • Author(s)
      Yoshida S, Inaba H, Murakami M, Yasuda H, Nakano K, Nomura R, Matsumoto-Nakano M, Nakano K.
    • Journal Title

      Journal of Oral Microbiology

      Volume: in press

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inhibition of Porphyromonas gulae and periodontal disease in dogs by a combination of clindamycin and interferon alpha2020

    • Author(s)
      Nomura R*, Inaba H*, Yasuda H*, Shirai M, Kato Y, Murakami M, Iwashita N, Shirahata S, Yoshida S, Matayoshi S, Yasuda J, Arai N, Asai F, Matsumoto-Nakano M, Nakano K. (*Contributed equally)
    • Journal Title

      Scientific reports

      Volume: 22 Issue: 12

    • DOI

      10.1111/cmi.13254

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibitory effect of a mouth rinse formulated with chlorhexidine gluconate, ethanol, and green tea extract against major oral bacterial species2020

    • Author(s)
      Nomura R, Inaba H, Matayoshi S, Yoshida S, Matsumi Y, Matsumoto-Nakano M, Nakano K
    • Journal Title

      Journal of Oral Science

      Volume: 10 Issue: 1 Pages: 206-21

    • DOI

      10.1038/s41598-020-63861-4

    • NAID

      130007821416

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification and functional analysis of glutamine transporter in Streptococcus mutans2020

    • Author(s)
      Morikawa Y, Morimoto S, Yoshida E, Naka S, Inaba H, Matsumoto-Nakano M
    • Journal Title

      Journal of Oral Microbiology

      Volume: 12 Issue: 1 Pages: 1797320-1797320

    • DOI

      10.1080/20002297.2020.1797320

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 口腔細菌が頭頸部癌細胞株に及ぼす影響と予後との関連性2022

    • Author(s)
      西山今日子、稲葉裕明、濱田 正和、吉田 翔、仲野 道代
    • Organizer
      第64回歯科基礎医学会学術大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 多嚢胞性脳軟化症患児口腔内における多数歯埋伏の1例2022

    • Author(s)
      仲周平、吉田翔、松三友紀、稲葉裕明、仲野道代
    • Organizer
      第41回日本小児歯科学会中四国地方会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Streptococcus mutansのコラーゲン結合タンパクCnmの構造解析2022

    • Author(s)
      松岡大貴、仲周平、稲葉裕明、仲野道代
    • Organizer
      第41回日本小児歯科学会中四国地方会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 非アルコール性脂肪肝炎患者口腔由来Streptococcus mutansの脂肪酸結合能の評価2022

    • Author(s)
      田畑佳子、中野聡大、仲周平、稲葉裕明、仲野道代
    • Organizer
      第41回日本小児歯科学会中四国地方会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Streptococcus mutans コラーゲン結合タンパク Cnm の構造および機能の検討2022

    • Author(s)
      松岡大貴,仲 周平,後藤花奈, 稲葉裕明,仲野道代
    • Organizer
      第43回岡山歯学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Porphyromonas gulae線毛のバイオフィルム形成能と遺伝子多型の関連2021

    • Author(s)
      吉田翔、稲葉裕明、仲野道代
    • Organizer
      第42回岡山歯学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 血清飢餓状態が頭頸部癌細胞株に及ぼす影響と飢餓誘導遺伝子の予後との関連性2021

    • Author(s)
      西山今日子、稲葉裕明、濱田 正和、吉田 翔、仲野 道代
    • Organizer
      第63回歯科基礎医学会学術大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Porphyromonas gulae 線毛遺伝子多型のバイオフィルム形成能への影響2021

    • Author(s)
      吉田翔、稲葉裕明、仲野道代
    • Organizer
      第63回歯科基礎医学会学術大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Porphyromonas gulae LPS 誘発炎症反応における緑茶ポリフェノールの抗炎症作用2021

    • Author(s)
      稲葉裕明、吉田翔、仲野道代
    • Organizer
      第63回歯科基礎医学会学術大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 岡山大学病院における抗菌薬適正使用支援チーム(AST)の取り組みと評価2021

    • Author(s)
      東恩納司、森下陽介、稲葉裕明、草野展周、塚原宏一
    • Organizer
      第36回日本環境感染学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 岡山大学病院での急性単純性膀胱炎に対する尿検査実施状況および経口抗菌薬の処方動向に関する実態調査2021

    • Author(s)
      森下陽介、東恩納司、稲葉裕明、草野展周、塚原宏一
    • Organizer
      第36回日本環境感染学会
    • Related Report
      2021 Research-status Report
  • [Presentation] Porphyromonas gulae プロテアーゼによる歯肉上皮細胞傷害能の評価2020

    • Author(s)
      吉田翔、稲葉裕明、Urmi Saki Alam、野村良太、仲野和彦、仲野道代
    • Organizer
      第58回日本小児歯科学会
    • Related Report
      2020 Research-status Report
  • [Presentation] The role of Porphyromonas gulae protease on bacterial growth and hemagglutination2020

    • Author(s)
      Urmi Saki Alam、 稲葉裕明、吉田翔、野村良太、仲野和彦、仲野道代
    • Organizer
      第58回日本小児歯科学会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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