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Development of control method for root caries focusing on the functions of oral biofilm microbiota

Research Project

Project/Area Number 20K09953
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57030:Conservative dentistry-related
Research InstitutionOsaka University

Principal Investigator

Asahi Yoko  大阪大学, 大学院歯学研究科, 助教 (50456943)

Co-Investigator(Kenkyū-buntansha) 三浦 治郎  大阪大学, 歯学部附属病院, 助教 (70437383)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsバイオフィルム / 根面う蝕 / 細菌叢 / 機能
Outline of Research at the Start

超高齢社会を迎え深刻な問題となっている根面う蝕に対しては,効果的な予防・制御法が切望されている。本研究では,初期根面う蝕に着目し,う蝕病変部の細菌叢を網羅的に遺伝子解析することで,根面う蝕の発症に関連する細菌叢および病原性を明らかにし,根面う蝕の発症初期における新規検出法を検討する。そして,得られる結果をもとに,初期根面う蝕病変を模するin situバイオフィルムモデルを用いて,初期根面う蝕に対する効果的な化学的制御法の確立を目指す。

Outline of Final Research Achievements

Biofilms were collected from root caries lesions, and the function of biofilm bacteria associated with root caries was investigated. The results suggested that microbiota associated with root caries lesion may have a different function from the biofilm formed on the healthy exposed root surface.
On the other hand, as a result of investigating the effects of zinc-glass-containing glass ionomer cement on biofilms, it was shown that the number of viable bacteria and the thickness of the biofilm of Actinomyces naeslundii decreased compared to the control group, and that gene expression changed.

Academic Significance and Societal Importance of the Research Achievements

高齢者の保有歯数が増加し,加齢や歯周病による歯肉退縮に伴う根面う蝕の急増が歯科において深刻な問題となっており,根面う蝕に対する効果的な予防・制御法が切望されている。本研究により,根面う蝕病変部によりバイオフィルム細菌叢の機能が変化する可能性が示された。得られた結果は,根面う蝕の病態解明や新たな抑制法の開発の一助となりうる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (11 results)

All 2024 2023 2022 2021

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Clinical investigation of the inhibitory effects of tooth-coating materials on initial active root caries: A pilot randomized controlled trial.2024

    • Author(s)
      Asahi Y, Naito K, Kanda H, Niwano K, Takegawa D, Yumoto H, Noiri Y, Hayashi M.
    • Journal Title

      Medicina

      Volume: 60 Issue: 1 Pages: 150-150

    • DOI

      10.3390/medicina60010150

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Repair of an Extensive External Cervical Resorption Lesion Using Intentional Replantation with Crown Rotation2023

    • Author(s)
      Okamoto Motoki、Asahi Yoko、Duncan Henry Fergus、Kuriki Nanako、Takahashi Yusuke、Hayashi Mikako
    • Journal Title

      Case Reports in Dentistry

      Volume: - Pages: 1-10

    • DOI

      10.1155/2023/2103999

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An extensive description of the microbiological effects of silver diamine fluoride on dental biofilms using an oral in situ model2022

    • Author(s)
      Klanliang K, Asahi Y, Maezono H, Sotozono M, Kuriki N, Machi H, Ebisu S, Hayashi M.
    • Journal Title

      Sci Rep .

      Volume: 12 Issue: 1 Pages: 7435-7435

    • DOI

      10.1038/s41598-022-11477-1

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impact of sleep on the microbiome of oral biofilms2021

    • Author(s)
      Sotozono Maki、Kuriki Nanako、Asahi Yoko、Noiri Yuichiro、Hayashi Mikako、Motooka Daisuke、Nakamura Shota、Yamaguchi Mikiyo、Iida Tetsuya、Ebisu Shigeyuki
    • Journal Title

      PLOS ONE

      Volume: 16 Issue: 12 Pages: e0259850-e0259850

    • DOI

      10.1371/journal.pone.0259850

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impacts of sleep on the characteristics of dental biofilm.2021

    • Author(s)
      Sotozono M, Kuriki N, Asahi Y, Noiri Y, Hayashi M, Motooka D, Nakamura S, Machi H, Iida T, Ebisu S.
    • Journal Title

      Sci Rep

      Volume: 11 Issue: 1 Pages: 138-138

    • DOI

      10.1038/s41598-020-80541-5

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] The antimicrobial effect of peroxynitric acid on polymicrobial biofilms.2023

    • Author(s)
      Maezono H, Shimaoka T, Ono S, Takahashi Y, Asahi Y, Kitano K, Ikawa S, Hayashi M
    • Organizer
      21st ESE Biennial Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 難治性根尖性歯周炎罹患歯の根尖部根管内と根尖孔外バイオフィルムにおける細菌叢の比較2023

    • Author(s)
      朝日陽子、栗木菜々子、前薗葉月、島岡 毅、恵比須繁之、野杁由一郎、林 美加子
    • Organizer
      第37回日本バイオフィルム学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 初期活動性根面う蝕患者におけるケアダインZIF-Cのバイオフィルム抑制効果に関する臨床評価2023

    • Author(s)
      朝日陽子、内藤克昭、神田ひかる、庭野和明、武川大輔、湯本浩通、野杁由一郎、林美加子
    • Organizer
      第159回日本歯科保存学会秋季学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] The inhibitory effect of on-demand aqueous chlorine dioxide solution on polymicrobial biofilm.2023

    • Author(s)
      Shimaoka T, Maezono H, Ono S, Kawanishi Y, Asahi Y, Takahashi Y, Hayasih M
    • Organizer
      21st ESE Biennial Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 要時生成型亜塩素酸イオン水溶液がEnterococcus faecalis バイオフィルムに及ぼす影響2022

    • Author(s)
      島岡 毅,前薗 葉月,川西 雄三,朝日 陽子,高橋 雄介,林 美加子
    • Organizer
      第157回 歯科保存学会2022年度秋季学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Effects of silver diamine fluoride on in situ oral biofilm2021

    • Author(s)
      Klanliang K, Asahi Y, Maezono H, Kuriki N, Shimaoka T, Machi H, Ebisu S, Hayashi M
    • Organizer
      第69回国際歯科研究学会日本部会(JADR)総会・学術大会
    • Related Report
      2021 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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