| Project/Area Number |
20K09969
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57030:Conservative dentistry-related
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高橋 富美 産業医科大学, 医学部, 教授 (50274436)
阿南 壽 福岡歯科大学, 口腔歯学部, 病院顧問 (80158732)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | sphingosine-1-phosphate / SCAP / differentiation / odontoblast / S1P / 歯乳頭由来幹細胞(SCAP) / 象牙芽細胞分化 / S1PR1シグナル / 再生歯内療法 / 歯乳頭由来幹細胞 / S1Pシグナル / 歯髄血管再生療法 |
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| Outline of Research at the Start |
失われた象牙質・歯髄の構造と機能をスフィンゴシン-1-リン酸 (S1P) シグナルの応用により早期に回復し、新しい歯髄血管再生療法を目指す。本研究は「歯を保存する」治療法開発へと繋がる研究である。
S1Pシグナルを介した歯乳頭由来幹細胞 (SCAP)の象牙芽細胞分化メカニズムの解明と、臨床実用化を目指したS1Pによる歯髄血管再生療法の検証を行う。
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| Outline of Final Research Achievements |
During odontogenic differentiation of mouse stem cells of the apical papilla (iSCAP), we found that S1P and BMP-9 induced mRNA and secreted protein expression of differentiation markers including DSPP, DMP1, and MEPE, and promoted mineralization through S1P/S1PR1 signaling pathway. In rat immature teeth, many S1PR1-positive cells were observed in the pulp tissue and the adjacent odontoblast-like cell area, as well as in the apical papilla. Furthermore, in the regenerative endodontic procedures model, an increase in root canal wall thickness and the formation of bone-like hard tissue near the root apex were observed due to the S1PR1 agonist.
S1P/S1PR1 pathway may be involved in promoting odontogenic differentiation of SCAP and root formation after regenerative endodontic procedures.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で見出したS1P/S1PRシグナルによる新たなSCAPの象牙芽細胞分化促進作用は、再生歯内療法後の歯根形成におけSCAPの賦活化と象牙質-歯髄複合体の再生に応用できる可能性がある。
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