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Solid-state NMR investigation on the mineral structure in de- and remineralized dentin. Solid-state NMR investigation to provide evidence of fluoride-effect for caries prevention

Research Project

Project/Area Number 20K09972
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57030:Conservative dentistry-related
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Hiraishi Noriko  東京医科歯科大学, 大学院医歯学総合研究科, 講師 (20567747)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsフッ化物 / フルオロアパタイト / 固体核磁気共鳴装置 / エナメル質 / 象牙質 / フッ化カルシウム / X線回折装置 / 核磁気共鳴装置 / 歯質ミネラル / う蝕予防 / X線回折 / 固体核磁気共鳴
Outline of Research at the Start

う蝕予防のためのフッ化物の応用は、世界の各国と比較し、日本は60%程度の低い普及率である。推奨のために、高度分析技術を応用したエビデンスを提供する必要があるが、フッ化物効果を、分子、原子レベルでの的確な分析は少なく、幼弱と成熟エナメル質での反応性の違い、健全象牙質と脱灰象牙質への効果の違いを検討した報告はない。結晶学分析的観点から、歯質ミネラルとの反応生成物を同定すべく、固体核磁気共鳴(NMR)測定法を応用する。ライフステージを考慮した、安全かつ有効な再石灰化機序に基づくフッ化物応用法を確立し、その進展を目指す。

Outline of Final Research Achievements

Various fluorides were applied to bovine and human enamel and dentin and the effect of fluoride treatment was assessed by solid-state NMR. Bovine tooth bioapatite was used in preliminary experiments and its crystallinity was investigated to compare with human tooth. Enamel was found to be a highly crystalline mineral. Rietveld analysis of X-ray diffraction confirmed smaller crystal sizes and greater microstrain in dentin. 19F NMR analysis of fluoride showed that it was highly reactive in dentin, producing fluoroapatite (FAp) and calcium fluoride (CaF2). Acid fluoride is reactive, but the by-product CaF2 is mainly formed. The formation of CaF2 was found by silver diammine fluoride as well as FAp. Solid-state NMR and XRD analysis allowed the action of fluoride on dentine apatite and the identification of its products, suggesting that the reactivity varies with the acidity and concentration of fluoride, providing new insights into its caries-preventive effect.

Academic Significance and Societal Importance of the Research Achievements

う蝕予防のためのフッ化物の応用については、エナメル質初期う蝕予防並みならず、成人期以降に見られる歯根面う蝕の予防においても、フッ化物の応用効果が期待される。前者には1450ppm以上のフッ化物、後者にはフッ化ジアンミン銀塗布が広く使用されるが、どのような生成物が生じるかを、固体NMRの高度分析技術を駆使して、分子、原子レベルでの的確な分析を行った。結果、酸性フッ化物は反応性が高いが、高濃度の場合、フルオロアパタイト以外にフッ化カルシウムが多く生成される傾向があった。エナメル質と象牙質で、フッ化物の反応性が違うため、臨床での使用にその特徴に合わせた使用方法が望ましいと考えた。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (37 results)

All 2024 2023 2022 2021 2020 Other

All Int'l Joint Research (1 results) Journal Article (19 results) (of which Int'l Joint Research: 16 results,  Peer Reviewed: 19 results,  Open Access: 8 results) Presentation (17 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results)

  • [Int'l Joint Research] Queen Mary University of London, London(英国)

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Effect of water glass treatment for zirconia and silane coupling on bond strength of resin cement2024

    • Author(s)
      Ushijima K, Hiraishi N, Ikeda M, Tsuji Y, Tsuchida Y, Shimada Y
    • Journal Title

      Clin Oral Investig

      Volume: 8 Issue: 6 Pages: 305-305

    • DOI

      10.1007/s00784-024-05680-9

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of borate, fluoride and strontium ions on biomimetic nucleation of calcium phosphate studied using solid-state nuclear magnetic resonance and X-ray diffraction2024

    • Author(s)
      Hiraishi N, Gondo T, Shimada Y, Hayashi F.
    • Journal Title

      Dent Mate

      Volume: 40 Issue: 2 Pages: 210-218

    • DOI

      10.1016/j.dental.2023.11.010

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of surface pre-reacted glass-ionomer (S-PRG) filler eluate on collagen morphology, remineralization, and ultimate tensile strength of demineralized dentin2023

    • Author(s)
      Ubolsa-Ard P, Sanon K, Hiraishi N, Sayed M, Sakamaki Y, Yiu CKY, Shimada Y
    • Journal Title

      J Mech Behav Biomed Mater

      Volume: 150 Pages: 106295-106295

    • DOI

      10.1016/j.jmbbm.2023.106295

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] In vitro remineralization assessment of enamel subsurface lesions using different percentages of surface reaction-type pre-reacted glass-ionomer containing gum-based material2023

    • Author(s)
      ThanNaing S, Hiraishi N, Chen X, Foxton R, Shimada Y
    • Journal Title

      J Dent

      Volume: 135 Pages: 4602-4602

    • DOI

      10.1016/j.jdent.2023.104602

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of an In-Office Bleaching Agent with Surface Pre-Reacted Glass-Ionomer Filler on the Enamel Surface: A In-Vitro Study2023

    • Author(s)
      Shimojima M, Hiraishi N, Akabane K, Nassar M, Otsuki M, Shimada Y
    • Journal Title

      J Funct Biomater

      Volume: 14 Issue: 7 Pages: 386-386

    • DOI

      10.3390/jfb14070386

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The bleaching effect of office bleaching agents containing S-PRG filler evaluated by pH value and electron spin resonance2023

    • Author(s)
      Akabane Kodai、Hiraishi Noriko、Shimojima Mika、Nassar Mohannad、Qi Feng、Otsuki Masayuki、Shimada Yasushi
    • Journal Title

      Clinical Oral Investigations

      Volume: - Issue: 7 Pages: 4051-4059

    • DOI

      10.1007/s00784-023-05031-0

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of Er: YAG laser irradiation with additional low energy on resin-dentin bonding and morphology of bonded interface2023

    • Author(s)
      Wei Diantong、Nakamoto Ayako、Hiraishi Noriko、Nakane Akira、Abuna Gabriel、Otsuki Masayuki、Shimada Yasushi
    • Journal Title

      Journal of the Mechanical Behavior of Biomedical Materials

      Volume: 140 Pages: 105692-105692

    • DOI

      10.1016/j.jmbbm.2023.105692

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Crystallographic and Physicochemical Analysis of Bovine and Human Teeth Using X-ray Diffraction and Solid-State Nuclear Magnetic Resonance2022

    • Author(s)
      Hiraishi Noriko、Gondo Tadamu、Shimada Yasushi、Hill Robert、Hayashi Fumiaki
    • Journal Title

      Journal of Functional Biomaterials

      Volume: 13 Issue: 4 Pages: 254-254

    • DOI

      10.3390/jfb13040254

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Raman Analyses of Laser Irradiation-Induced Microstructural Variations in Synthetic Hydroxyapatite and Human Teeth2022

    • Author(s)
      Imamura Hayata、Zhu Wenliang、Adachi Tetsuya、Hiraishi Noriko、Marin Elia、Miyamoto Nao、Yamamoto Toshiro、Kanamura Narisato、Pezzotti Giuseppe
    • Journal Title

      Journal of Functional Biomaterials

      Volume: 13 Issue: 4 Pages: 200-200

    • DOI

      10.3390/jfb13040200

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of Surface Pre-Reacted Glass Ionomer Containing Dental Sealant on the Inhibition of Enamel Demineralization2022

    • Author(s)
      Ogawa Yuko、Sayed Mahmoud、Hiraishi Noriko、Al-Haj Husain Nadin、Tagami Junji、?zcan Mutlu、Shimada Yasushi
    • Journal Title

      Journal of Functional Biomaterials

      Volume: 13 Issue: 4 Pages: 189-189

    • DOI

      10.3390/jfb13040189

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Solid-state NMR spectroscopy measurement of fluoride reaction by bovine enamel and dentin treated with silver diammine fluoride2022

    • Author(s)
      Hiraishi Noriko、Sayed Mahmoud、Hill Robert、Shimada Ysushi
    • Journal Title

      Dental Materials

      Volume: 38 Issue: 5 Pages: 769-777

    • DOI

      10.1016/j.dental.2022.04.017

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Clinical and primary evidence of silver diamine fluoride on root caries management2022

    • Author(s)
      Hiraishi Noriko、Sayed Mahmoud、Takahashi Motoi、Nikaido Toru、Tagami Junji
    • Journal Title

      Japanese Dental Science Review

      Volume: 58 Pages: 1-8

    • DOI

      10.1016/j.jdsr.2021.11.002

    • NAID

      210000177947

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of silver diamine fluoride preparations on biofilm formation of <i>Streptococcus mutans </i>2021

    • Author(s)
      TAKAHASHI Motoi、MATIN Khairul、MATSUI Naoko、SHIMIZU Miyuki、TSUDA Yuka、UCHINUMA Shigeki、HIRAISHI Noriko、NIKAIDO Toru、TAGAMI Junji
    • Journal Title

      Dental Materials Journal

      Volume: 40 Issue: 4 Pages: 911-917

    • DOI

      10.4012/dmj.2020-341

    • NAID

      130008069848

    • ISSN
      0287-4547, 1881-1361
    • Year and Date
      2021-07-25
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Interactions of boron released from surface pre-reacted glass ionomer with enamel/dentin and its effect on pH2021

    • Author(s)
      Hiraishi Noriko、Sayed Mahmoud、Hill Robert、Tagami Junji、Hayashi Fumiaki
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 1-8

    • DOI

      10.1038/s41598-021-95279-x

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dentin anti-demineralization potential of surface reaction-type pre-reacted glass-ionomer filler containing self-adhesive resin cement2021

    • Author(s)
      ThanNaing SoeKayThwe、Abdou Ahmed、Sayed Mahmoud、Sumi Yasunori、Tagami Junji、Hiraishi Noriko
    • Journal Title

      Clinical Oral Investigations

      Volume: 26 Issue: 2 Pages: 1333-1342

    • DOI

      10.1007/s00784-021-04107-z

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of smear layer removal agents on the microhardness and roughness of radicular dentin2021

    • Author(s)
      Muana Hosea Lalrin、Nassar Mohannad、Dargham Ahmad、Hiraishi Noriko、Tagami Junji
    • Journal Title

      The Saudi Dental Journal

      Volume: 33 Issue: 7 Pages: 661-665

    • DOI

      10.1016/j.sdentj.2020.05.001

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The effect of surface pre‐reacted glass‐ionomer filler eluate on dental pulp cells and mineral deposition on dentin: In vitro study2021

    • Author(s)
      Nishimaki Mayuri、Nassar Mohannad、Tamura Yukihiko、Hiraishi Noriko、Dargham Ahmad、Nikaido Toru、Tagami Junji
    • Journal Title

      European Journal of Oral Sciences

      Volume: 129 Issue: 3 Pages: 1-8

    • DOI

      10.1111/eos.12777

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Evaluation of tooth demineralization and interfacial bacterial penetration around resin composites containing surface pre-reacted glass-ionomer (S-PRG) filler2021

    • Author(s)
      Zhou Yuan、Hiraishi Noriko、Shimada Yasushi、Wang Guoqing、Tagami Junji、Feng Xiping
    • Journal Title

      Dental Materials

      Volume: 37 Issue: 5 Pages: 849-862

    • DOI

      10.1016/j.dental.2021.02.009

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The effect of reduced glutathione on the toxicity of silver diamine fluoride in rat pulpal cells2021

    • Author(s)
      KIM Seunggun、NASSAR Mohannad、TAMURA Yukihiko、HIRAISHI Noriko、JAMLEH Ahmed、NIKAIDO Toru、TAGAMI Junji
    • Journal Title

      Journal of Applied Oral Science

      Volume: 29 Pages: 1-8

    • DOI

      10.1590/1678-7757-2020-0859

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Scientific Evidence on Restorative Ability of S-PRG Filler for Enamel and Dentin2024

    • Author(s)
      Noriko Hiraishi
    • Organizer
      Industry-sponsored Symposium (Invited speaker) The 102nd 2024 IADR/AADOCR/CADR General Session & Exhibition the IADR/APR General Session & Exhibition
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 19F-NMR Study of Fluoride Reaction of Various Formulations on Enamel2024

    • Author(s)
      Noriko Hiraishi, Fumiaki Hayashi Tadamu Gondo, Toru Nikaido, Yasushi Shimada
    • Organizer
      The 102nd 2024 IADR/AADOCR/CADR General Session & Exhibition the IADR/APR General Session & Exhibition
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparative Analysis of the Coronal and Root Caries Microbiome2024

    • Author(s)
      Tadamu Gondo, Noriko Hiraishi, Azusa Takeuchi, David Moyes, Yasushi Shimada
    • Organizer
      The 102nd 2024 IADR/AADOCR/CADR General Session & Exhibition the IADR/APR General Session & Exhibition
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chemical, Biological, and Mechanical Properties of a Novel Glass Ionomer2024

    • Author(s)
      Nader Ahmed Elseoudy, Kittisak Sanon2, Noriko Hiraishi, Yukihiko Tamura, Yasushi Shimada
    • Organizer
      The 102nd 2024 IADR/AADOCR/CADR General Session & Exhibition the IADR/APR General Session & Exhibition
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Restorative Ability of S-PRG Filler Preserves Enamel and Dentin2023

    • Author(s)
      Noriko Hiraishi
    • Organizer
      Meet the Industry Session - Shofu Inc. ADM
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Zirconia adhesion of phosphoric acid ester monomers: A solid-state NMR investigation2023

    • Author(s)
      平石典子,牛島 啓,池田正臣,島田康史
    • Organizer
      第88回口腔病学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Effect of Er:YAG laser debonding of zirconia restorations on bond strength, temperature increase and surface properties of zirconia and dentin2023

    • Author(s)
      Diantong Wei, Jianfeng Chen, Noriko Hiraishi, Masayuki Otsuki, Masaomi Ikeda
    • Organizer
      第88回口腔病学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] S-PRGフィラー含有3Dプリント樹脂のイオン放出量とその物性評価について2023

    • Author(s)
      辻悠貴子,平石典子,池田正臣,宇尾基弘,二階堂徹,島田康史
    • Organizer
      第159回日本歯科保存学会秋季学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 牛歯とヒト抜去歯のX線回折におけるリートベルト解析による結晶学的比較2022

    • Author(s)
      藤理夢, 平石典子, 島田康史
    • Organizer
      第87回口腔病学会学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 歯冠・根面う蝕からの細菌叢DNAの抽出と細菌構成の比較2022

    • Author(s)
      権藤理夢、平石典子、黒厚子璃佳、岩本勉、島田康史
    • Organizer
      第157回日本歯科保存学会秋季学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 水ガラス浸透ジルコニアとレジンセメントとの引張り接着強さ2022

    • Author(s)
      牛島啓, 平石典子, 池田正臣, 島田康史
    • Organizer
      第157回日本歯科保存学会秋季学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] S-PRGフィラー配合によるオフィス漂白剤のエナメル質に及ぼす影響2022

    • Author(s)
      下島美夏, 平石典子, 赤羽根広大, Feng QI, 大槻昌幸, 島田康史
    • Organizer
      第156回日本歯科保存学会春季学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] SーPRGフィラー配合によるオフィスブリーチ剤の漂白効果2022

    • Author(s)
      赤羽根広大, 平石典子, 下島美夏, Feng QI, 大槻昌幸, 島田康史
    • Organizer
      第156回日本歯科保存学会春季学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Bioactive Effect of S-PRG released ions on mineral precipitation and reaction with enamel and dentin2022

    • Author(s)
      Noriko Hiraishi
    • Organizer
      International Dental Materials Congress
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 19F and 31P solid state NMR study on fluoridate apatite in human dental calculus2021

    • Author(s)
      Noriko Hiraishi, Fumikai Hayashi, Junji Tagami
    • Organizer
      The 98th, the IADR/AADR/CADR General Session & Exhibition
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] セルフエッチングプライマー採用S-PRGフィラー含有シーラントによる、エナメル質脱灰/再石灰化への影響2021

    • Author(s)
      小川友子、平石典子、田上順次
    • Organizer
      第154回日本歯科保存学会春季学術大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 歯石に含まれるフッ素性アパタイトの19F-MASおよび 1H-31P CP/MAS固体NMRによる解析2020

    • Author(s)
      小川友子、林文晶、平石典子、田上順次
    • Organizer
      日本歯科保存学会学術大会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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