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Analysis of the function of Cdc42 in the maintenance and defense mechanisms of gingival junction epithelium.

Research Project

Project/Area Number 20K09978
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57030:Conservative dentistry-related
Research InstitutionShowa University

Principal Investigator

Aizawa Ryo  昭和大学, 歯学部, 助教 (80710673)

Co-Investigator(Kenkyū-buntansha) 田中 準一  昭和大学, 歯学部, 講師 (40710166)
美島 健二  昭和大学, 歯学部, 教授 (50275343)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsCdc42 / 不死化接合上皮細胞 / 接合上皮
Outline of Research at the Start

歯周病は口腔内の歯周病原細菌に対する宿主の反応により歯周組織の破壊を伴う感染症で
ある。歯肉接合上皮は細菌の侵入に対して最前線に位置し、物理的な防御の役割を果たして
いる。Rhoファミリータンパク質Cdc42が接合上皮の維持、防御機能において果たしている機能は明らかになっていない。本研究の目的は、マウス由来の不死化接合上皮細胞を用いて、Cdc42が接合上皮における創傷治癒、歯周病制御に果たす役割を明らかにすることである。

Outline of Final Research Achievements

In this study, we investigated the function of Cdc42 in the protective state of junctional epithelium against periodontitis using immortalized junctional epithelial cells JE-1. Stimulation of JE-1 with LPS derived from Porphyromonas gingivalis was shown to decrease cell proliferation and migration capacity. Examination of gene expression showed that LPS increased the expression of Cdc42, Cxcl2 and Slpi, and decreased the expression of Cxcl10, Cdh1 and Itgb4 in JE-1. These results suggest that Cdc42 may also be involved in enhancing the defense of junctional epithelial cells against periodontal pathogenic bacteria by regulating cytoskeleton and cell motility and increasing intercellular permeability.

Academic Significance and Societal Importance of the Research Achievements

Cdc42と歯周病に着目した研究はこれまでほとんどなく、特に接合上皮細胞におけるCdc42の役割は明らかでなかった。本研究結果から、接合上皮の歯周炎に対する防御機能において、Cdc42が重要な役割を果たしていることが示唆された。今後、Cdc42に着目した歯周炎の予防および治療に有用な手法を見出せる可能性がある。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (15 results)

All 2022 2021 2020 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (5 results) (of which Int'l Joint Research: 1 results) Remarks (5 results)

  • [Journal Article] Mechanisms of junctional epithelial homeostasis revealed by the accumulation of knowledge2022

    • Author(s)
      相澤 怜,田中準一,美島健二,山本松男
    • Journal Title

      Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)

      Volume: 64 Issue: 4 Pages: 121-128

    • DOI

      10.2329/perio.64.121

    • ISSN
      0385-0110, 1880-408X
    • Year and Date
      2022-12-28
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Role of Lactic Acid on Wound Healing, Cell Growth, Cell Cycle Kinetics, and Gene Expression of Cultured Junctional Epithelium Cells in the Pathophysiology of Periodontal Disease2021

    • Author(s)
      Taichi Ishikawa、Daisuke Sasaki、Ryo Aizawa、Matsuo Yamamoto、Takashi Yaegashi、Tarou Irie、Minoru Sasaki
    • Journal Title

      Pathogens

      Volume: 10 Issue: 11 Pages: 1507-1507

    • DOI

      10.3390/pathogens10111507

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure of junctional epithelium is maintained by cell populations supplied from multiple stem cells2021

    • Author(s)
      Tanaka Keisuke、Tanaka Junichi、Aizawa Ryo、Kato-Tanaka Mayu、Ueno Hiroo、Mishima Kenji、Yamamoto Matsuo
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 18860-18860

    • DOI

      10.1038/s41598-021-98398-7

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of Butyric Acid in the Proliferation and Migration of Junctional Epithelium in the Progression of Periodontitis: An In Vitro Study2021

    • Author(s)
      Taichi Ishikawa、Daisuke Sasaki、Ryo Aizawa、Yu Shimoyama、Matsuo Yamamoto、Tarou Irie、 Minoru Sasaki
    • Journal Title

      Dentistry Journal

      Volume: 9 Issue: 4 Pages: 44-44

    • DOI

      10.3390/dj9040044

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Maintaining a protective state for human periodontal tissue2021

    • Author(s)
      Yamamoto Matsuo、Aizawa Ryo
    • Journal Title

      Periodontology 2000

      Volume: ― Issue: 1 Pages: 142-156

    • DOI

      10.1111/prd.12367

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] Porphyromonas gingivalis由来LPSが不死化接合上皮細胞の細胞機能に及ぼす影響.2022

    • Author(s)
      相澤 怜,田中慧介,田中準一,菅野真莉加,美島健二,山本松男
    • Organizer
      第65回秋季日本歯周病学会学術大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 接合上皮細胞は推定上の幹細胞により供給される2021

    • Author(s)
      田中慧介,田中準一,相澤 怜,田中麻友,上野博夫,美島健二,山本松男
    • Organizer
      第64回秋季日本歯周病学会学術大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 多色細胞系譜追跡法を用いた歯肉接合上皮幹細胞様細胞の探索2020

    • Author(s)
      田中慧介,田中準一,相澤 怜,田中麻友,美島健二,山本松男
    • Organizer
      第67回昭和大学学士会総会
    • Related Report
      2020 Research-status Report
  • [Presentation] Investigation of junctional epithelial stem/progenitor cells using the multicolor lineage tracing method2020

    • Author(s)
      Tanaka K, Tanaka J, Aizawa R, Kato-Tanaka M, Ueno H, Mishima K, Yamamoto M
    • Organizer
      第106回アメリカ歯周病学会共催日本歯周病学会・日本臨床歯周病学会2020年大会
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] 多色細胞系譜追跡法を用いた歯肉接合上皮細胞のクローナリティ解析2020

    • Author(s)
      田中慧介,田中準一,相澤 怜,吉田真子,松浦 徹,藤田恭平,田中麻友,上野博夫,美島健二,山本松男
    • Organizer
      第63回秋季日本歯周病学会学術大会
    • Related Report
      2020 Research-status Report
  • [Remarks] 昭和大学歯学部歯科保存学講座歯周病学部門

    • URL

      http://www10.showa-u.ac.jp/~perio/index.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] 昭和大学 研究者情報・業績集

    • URL

      https://rira.showa-u.ac.jp/search/index.html;jsessionid=2065C03E8A005DD2B96A6BA9CF9E5DE4?lang=ja

    • Related Report
      2022 Annual Research Report
  • [Remarks] 昭和大学歯学部歯周病学講座

    • URL

      http://www10.showa-u.ac.jp/~perio/index.html

    • Related Report
      2021 Research-status Report 2020 Research-status Report
  • [Remarks] 昭和大学研究者情報・業績集

    • URL

      https://researchers-achievements.showa-u.ac.jp

    • Related Report
      2021 Research-status Report
  • [Remarks] 昭和大学研究者情報・業績集

    • URL

      https://researchers-achievements.showa-u.ac.jp/

    • Related Report
      2020 Research-status Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

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