| Project/Area Number |
20K11531
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 59040:Nutrition science and health science-related
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岩間 久和 香川大学, 医学部, 准教授 (20398035)
正木 勉 香川大学, 医学部, 教授 (30335848)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 非アルコール性脂肪肝炎 / 体組成計測 / マイクロRNA / 非アルコール性脂肪性肝疾患 / 重症化予測 |
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| Outline of Research at the Start |
非アルコール性脂肪性肝疾患（NAFLD）、およびその重症型である非アルコール性脂肪肝炎（NASH)と診断された症例で体組成計測と血清miRNAの網羅的解析を行い、体組成型別にNASH特異的に発現するmiRNA分子種を同定し、重症化予測アルゴリズムを構築する。本研究はNASH/NAFLDの中心病態を反映する体組成という栄養科学的なパラーメータを導入し、新世代型バイオマーカーのmiRNAと組み合わせる事で従来困難であった正確なNASH発症予測を目指す。
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| Outline of Final Research Achievements |
We aimed to develop a simple diagnostic method to select high-risk groups for nonalcoholic steatohepatitis (NASH), which is associated with a high rate of liver-related deaths, from among patients with nonalcoholic fatty liver disease (NAFLD), and to establish a method for predicting the onset of NASH by combining body composition measurement and serum microRNA (miRNA) measurement.
We performed body composition measurements using bioelectrical impedance analysis on NAFLD/NASH patients who were pathologically diagnosed at our institution, and simultaneously performed comprehensive analysis of serum miRNAs in the patients. Body composition was classified into five types, and the expression intensity of each miRNA was compared between NASH and non-NASH groups. Several miRNA molecular species were identified whose expression intensity was specifically changed in NASH cases, and it was shown that this tendency was stronger in certain body composition types.
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| Academic Significance and Societal Importance of the Research Achievements |
NASHの早期診断・発症予測の研究は全世界で行われ、最新の血清バイオマーカーや画像検査法が数多く提案されているが感度・特異度に問題がある。その最大の原因は本疾患の発症進展に最大の影響を及ぼす患者体組成の観点を欠いている事であると思われる。短時間・簡便に行える体組成計測による栄養学的視点を血清miRNA解析に導入した本研究の成果はNASH診断・予測の研究分野に大きなインパクトをもたらすと考える。
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