| Project/Area Number |
20K11532
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 59040:Nutrition science and health science-related
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| Research Institution | 株式会社レオロジー機能食品研究所 |
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Principal Investigator |
Niwase Shamim 株式会社レオロジー機能食品研究所, 未登録, 基礎研究部長 (40647707)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | GPCR61 / Plasmalogens / Adiponectin / Fatty liver / Intestinal cells / Central nervous system / plasmalogens / RNASeq / GPR61 / Lipid metabolism / Epithelial cells / AMPK / PPAR |
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| Outline of Research at the Start |
Plsの事前経口投与により、2型糖尿病発症ZDF（Zucker Diabetic Fatty）ラットの糖尿病の発症をおさえ、脂肪肝の発生を抑制することを確認している（データ未発表）。この予防効果に対して本研究では、発症後のZDFラットにPlsを投与して、血中 アディポネクチンを上昇させ、脂肪肝を減衰させる治療型アプローチで検証を行う。また、 マウス細胞と、GPR61全身ノックアウトマウス、組織特異的GPR61ノックアウトマウス、糖 尿病モデルマウスを用いてGPR61がアディポネクチンに作用する機序と、Plsによる抗糖尿病 効果検証を行う。
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| Outline of Final Research Achievements |
GPCR61 showed to regulate the expression of Adiponectin in intestinal cells and the oral ingestion of plasmalogens (Pls), which is the ligands of GPCR61 induced expression of Apiponectin, suggesting that Pls-GPCR61 signaling enhance adiponectin to reduce diabetic syndrome such as fat-deposition in the tissues including liver. WE successfully generated GPCR61 knockout (KO) mice which showed increased body weight marked with fatty liver. Next generation sequencing (RNASeq) data using the GPCR61 revealed a comprehensive mechanism of GPCR61 signaling in mice tissues including brain and immune cells. Our present research provided important clues in understanding the role of GPCR61 not only in the intestine but also in the brain tissues and immune cells.
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| Academic Significance and Societal Importance of the Research Achievements |
This research provides a possible mechanism how we are susceptible to diabetes and other diseases during aging process when GPCR61 and plasmalogens are reduced. Therefore, this study could help in finding future therapeutics to cure various aging-related diseases including diabetes.
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