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Degradation of sulfur compounds by heterotrophic microorganisms and associated formation of corrosive gas emission

Research Project

Project/Area Number 20K12202
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 63040:Environmental impact assessment-related
Research InstitutionIndependent Administrative Institution National Institutes for Cultural Heritage Tokyo National Research Institute for Cultural Properties

Principal Investigator

KATAYAMA Yoko  独立行政法人国立文化財機構東京文化財研究所, 保存科学研究センター, 客員研究員 (90165415)

Co-Investigator(Kenkyū-buntansha) 佐藤 嘉則  独立行政法人国立文化財機構東京文化財研究所, 保存科学研究センター, 室長 (50466645)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords微生物代謝 / 真菌類 / 細菌類 / 気体状硫黄化合物 / 硫化カルボニル / 微生物劣化 / 土壌微生物 / 腐食性硫黄化合物 / 生物劣化 / 硫黄循環 / チオシアネート加水分解酵素
Outline of Research at the Start

大気の硫黄化合物の中で最大の存在量であり、しかも腐食性である硫化カルボニル(COS)の消長に関わる新規代謝経路の存在を、細菌及び真菌類について明らかにすることを目的とする。COS分解は関連酵素も含めて研究が進んでいるのに対し、COS放出に関する情報は限られている。代表者は真菌において未知のCOS発生の現象を見つけたことから、同様の反応は土壌でのCOS動態に深く関わるものとの仮説を立てた。本研究では、硫黄代謝活性を有する細菌並びに真菌の分離株を用い、COS発生のメカニズムを明らかにすると共に、微生物を発生源とするCOSの生物劣化被害低減に向けた基礎的知見を研究期間内に得る。

Outline of Final Research Achievements

The microbial emission and degradation of carbonyl sulfide (COS) was studied in fungi and bacteria. Although fungi isolated from soil generally show high COS-degrading activity, strains THIF09 and THIF13 and their relatives released COS, clearly indicating that information on COS-emitting fungi is important for understanding COS dynamics in the environments. A high frequency of COS-degrading bacteria was found in heterotrophic bacteria isolated from detached fragments collected from normally closed decorative tombs, indicating that COS-degrading bacteria are widespread in tombs. The genome of Thiobacillus thioparus strain THI115, in which SCNase and COSase were first discovered, was determined and genes involved in sulfur metabolism and chemolithoautotrophic growth were identified.

Academic Significance and Societal Importance of the Research Achievements

COSは成層圏エアロゾルの原因物質のひとつであり、気候変動の予測にCOSの動態情報は欠かせない。COSの放出が確認された真菌は土壌に広く分布するものであり、今後は土壌微生物によるCOS放出も含めた議論が重要であることを明示した。密閉環境から分離された従属栄養性細菌に高頻度でCOS分解と硫化水素の放出が確認され、顔料などへの影響の可能性が考えられる。T. thioparus THI115は硫黄代謝に関する新規酵素が発見された菌株であり、本菌株のゲノム情報は今後の研究において極めて重要な研究ツールとなる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (25 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (1 results) Journal Article (13 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 13 results,  Open Access: 6 results) Presentation (8 results) Book (2 results) Remarks (1 results)

  • [Int'l Joint Research] Guangdong Technion Israel Inst Technol(中国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] De-calcification as an important mechanism in (bio)deterioration of sandstone of Angkor monuments in Cambodia.2022

    • Author(s)
      Qian Youfen, Gan Tongzhou, Zada Sahib, Katayama Yoko, Gu Ji-Dong
    • Journal Title

      International Biodeterioration & Biodegradation

      Volume: 174 Pages: 105470-105470

    • DOI

      10.1016/j.ibiod.2022.105470

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microbiome characteristics and the key biochemical reactions identified on stone world cultural heritage under different climate conditions.2022

    • Author(s)
      Ding Xinghua, Lan Wensheng, Yan Aixin, Li Yiliang, Katayama Yoko, Gu Ji-Dong
    • Journal Title

      Journal of Environmental Management

      Volume: 302 Pages: 114041-114041

    • DOI

      10.1016/j.jenvman.2021.114041

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Draft genome sequences of Sulfurovum spp. TSL1 and TSL6, two sulfur-oxidizing bacteria isolated from marine sediment.2022

    • Author(s)
      Guo Yong, Ihara Hideyuki, Aoyagi Tomo, Hori Tomoyuki, Katayama Yoko
    • Journal Title

      Microbiology Resource Announcements

      Volume: 11 Issue: 1 Pages: 105488-105488

    • DOI

      10.1128/mra.00922-21

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] An internal recycling mechanism between ammonia/ammonium and nitrate driven by ammonia-oxidizing archaea and bacteria (AOA, AOB, and Comammox) and DNRA on Angkor sandstone monuments2021

    • Author(s)
      Ding Xinghua、Lan Wensheng、Li Yiliang、Yan Aixin、Katayama Yoko、Koba Keisuke、Makabe Akiko、Fukushima Keitaro、Yano Midori、Onishi Yuji、Ge Qinya、Gu Ji-Dong
    • Journal Title

      International Biodeterioration & Biodegradation

      Volume: 165 Pages: 105328-105328

    • DOI

      10.1016/j.ibiod.2021.105328

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Nitrate-Driven Trophic Association of Sulfur-Cycling Microorganisms in Tsunami-Deposited Marine Sediment Revealed by High-Sensitivity <sup>13</sup>C-Bicarbonate Probing2021

    • Author(s)
      Aoyagi Tomo、Katayama Yoko、Aizawa Hidenobu、Takasaki Mitsuru、Hori Tomoyuki
    • Journal Title

      Environmental Science & Technology

      Volume: 55 Issue: 12 Pages: 8410-8421

    • DOI

      10.1021/acs.est.0c08191

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Direct comparison of bacterial communities in soils contaminated with different levels of radioactive cesium from the first Fukushima nuclear power plant accident2021

    • Author(s)
      Ihara Hideyuki、Kumagai Ayako、Hori Tomoyuki、Nanba Kenji、Aoyagi Tomo、Takasaki Mitsuru、Katayama Yoko
    • Journal Title

      Science of The Total Environment

      Volume: 756 Pages: 143844-143844

    • DOI

      10.1016/j.scitotenv.2020.143844

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fungal Carbonyl Sulfide Hydrolase of <i>Trichoderma harzianum</i> Strain THIF08 and Its Relationship with Clade D β-Carbonic Anhydrases2021

    • Author(s)
      Masaki Yoshihito、Iizuka Ryuka、Kato Hiromi、Kojima Yuka、Ogawa Takahiro、Yoshida Makoto、Matsushita Yasuhiko、Katayama Yoko
    • Journal Title

      Microbes and Environments

      Volume: 36 Issue: 2 Pages: n/a

    • DOI

      10.1264/jsme2.ME20058

    • NAID

      130008041756

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increase in sedimentary organic carbon with a change from hypoxic to oxic conditions2021

    • Author(s)
      Mahmood Mukseet、Taki Shunsuke、Nakai Satoshi、Gotoh Takehiko、Nishijima Wataru、Umehara Akira、Aoyagi Tomo、Sato Yuya、Hori Tomoyuki、Katayama Yoko、Hajdu-Rahkama Reka、Puhakka Jaakko A.
    • Journal Title

      Marine Pollution Bulletin

      Volume: 168 Pages: 112397-112397

    • DOI

      10.1016/j.marpolbul.2021.112397

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] The active microbes and biochemical processes contributing to deterioration of Angkor sandstone monuments under the tropical climate in Cambodia-A review2021

    • Author(s)
      Li Jing、Deng Maocheng、Gao Lin、Yen Sufen、Katayama Yoko、Gu Ji-Dong
    • Journal Title

      Journal of Cultural Heritage

      Volume: 47 Pages: 218-226

    • DOI

      10.1016/j.culher.2020.10.010

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enumeration of Chemoorganotrophic Carbonyl Sulfide (COS)-degrading Microorganisms by the Most Probable Number Method2020

    • Author(s)
      Kato Hiromi, Ogawa Takahiro, Ohta Hiroyuki, Katayama Yoko
    • Journal Title

      Microbes and Environments

      Volume: 35 Issue: 2 Pages: n/a

    • DOI

      10.1264/jsme2.ME19139

    • NAID

      130007837480

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microbial diversity and composition of the Preah Vihear temple in Cambodia by high-throughput sequencing based on genomic DNA and RNA2020

    • Author(s)
      Meng Han, Zhang Xiaofeng, Katayama Yoko, Ge Qinya, Gu Ji-Dong
    • Journal Title

      International Biodeterioration & Biodegradation

      Volume: 149 Pages: 104936-104936

    • DOI

      10.1016/j.ibiod.2020.104936

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microbial deterioration and sustainable conservation of stone monuments and buildings2020

    • Author(s)
      Liu Xiaobo, Koestler Robert J, Warscheid Thomas, Katayama Yoko, Gu Ji-Dong
    • Journal Title

      Nature Sustainability

      Volume: 3 Issue: 12 Pages: 991-1004

    • DOI

      10.1038/s41893-020-00602-5

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microbiome and nitrate removal processes by microorganisms on the ancient Preah Vihear temple of Cambodia revealed by metagenomics and N-15 isotope analyses2020

    • Author(s)
      Ding Xinghua, Lan Wenshen, Wu Jiapeng, Hong Yiguo, Li Yiliang, Ge Qingya, Urzi Clara, Katayama Yoko, Gu Ji-Dong
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 104 Issue: 22 Pages: 9823-9837

    • DOI

      10.1007/s00253-020-10886-4

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 真菌類のCOS分解に関する生理学的および進化系統学的観点からの新たな知見2023

    • Author(s)
      飯塚瑠翔、吉田誠、服部祥平、片山葉子、大津巌生、David Hibbett
    • Organizer
      第73回日本木材学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 褐色腐朽菌Gloeophyllum trabeumにおける気体状硫化カルボニル代謝経路の調査2022

    • Author(s)
      飯塚瑠翔、吉田誠、梅澤究、大津巌生、片山葉子
    • Organizer
      日本木材保存協会 第38回年次大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 糸状菌における気体状硫化カルボニルを基質とする硫黄獲得経路に関する研究2022

    • Author(s)
      飯塚瑠翔、正木啓仁、小嶋由香、吉田誠、片山葉子、大津巌生
    • Organizer
      第72回日本木材学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 劣化した焼成煉瓦における微生物調査2021

    • Author(s)
      河﨑衣美、片山葉子、松井敏也、結城雅則、川本真由美
    • Organizer
      日本文化財科学会第38回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 虎塚古墳の壁画剥落片に形成された独特な微生物叢2021

    • Author(s)
      松野美由樹、片山葉子、犬塚将英、稲田健一、矢島國雄、佐藤嘉則
    • Organizer
      日本文化財科学会第38回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 糸状菌の硫黄代謝におけるガス状硫化カルボニルを介した代謝系に関する研究2021

    • Author(s)
      飯塚瑠翔、小坂優介、正木啓仁、吉田誠、片山葉子、大津巌生
    • Organizer
      第37回日本木材保存協会年次大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 木材腐朽菌による硫化カルボニル分解挙動の調査2020

    • Author(s)
      飯塚瑠翔、小坂優介、吉田誠、片山葉子、大津巌生
    • Organizer
      日本木材保存協会第36回年次大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 虎塚古墳の壁画剥落片から分離された微生物の群集構造解析2020

    • Author(s)
      松野美由樹、片山葉子、犬塚将英、稲田健一、矢島國雄、 佐藤嘉則
    • Organizer
      日本文化財化学会第37回大会
    • Related Report
      2020 Research-status Report
  • [Book] 富士山測候所のはなし-日本一高いところにある研究施設2022

    • Author(s)
      佐々木一哉、片山葉子、松田千夏、土器屋由紀子(共編)
    • Total Pages
      252
    • Publisher
      成山堂書店
    • ISBN
      9784425514816
    • Related Report
      2022 Annual Research Report
  • [Book] Microorganisms in the deterioration and preservation of cultural heritage2021

    • Author(s)
      Gu Ji-Dong, Katayama Yoko
    • Total Pages
      367
    • Publisher
      Springer, Cham
    • Related Report
      2021 Research-status Report
  • [Remarks] 文化財の生物劣化の現象解明と対策に関する研究:第4期中期計画 平成28年度-令和2年度

    • URL

      http://doi.org/10.18953/00009007

    • Related Report
      2020 Research-status Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

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