Novel label-free tool for infections diagnosis based on Nano-Electro Optical Tweezers
| Project/Area Number |
20K12701
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90130:Medical systems-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
KOTSIFAKI Domna 沖縄科学技術大学院大学, 量子技術のための光・物質相互作用ユニット, 客員研究員 (70834117)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Raman Spectroscopy / Nano-apertures / Metallic devices / Bacteria / Plasmonics / Optical Tweesers / Optical Tweezers / Nanoholes / Convection effects / phototoxicity / Optical trapping / Biomedicine / Metamaterials |
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| Outline of Research at the Start |
The interest of single-bacterium analysis has recently gained significant attention.In this proposal, the analysis and monitoring of antibiotic susceptibility at the single-bacterium level, in real-time, will be investigated via a novel platform named Electro-Nanohole Tweezers (ENT). I envision that ENT will be a high potential candidate to supplement or replace existing time-consuming methods such as antimicrobial resistance tests and will help the study to mitigate the challenge of drug resistance in clinical microbiology.
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| Outline of Annual Research Achievements |
By employing the unique electromagnetic properties of metamaterials, this year I focused on integrating Fano-resonant metamolecules to enhance Raman scattering signals from bacterial components. By exploiting off-resonance laser excitation and local immobilization of microorganisms on metamaterial surfaces, I have overcome existing challenges associated with weak Raman signals and photodamage effects, enabling robust detection even in complex liquid matrices. This part of the project involved the design, fabrication, and optimization of Fano-enhanced Raman scattering platforms tailored for microbial detection. Experimental studies were conducted using Escherichia coli as a model microorganism, with Raman signatures recorded at various growth phases. I have cultured two different solutions with bacteria in mid-exponential phase and stationary phase. The time-dependent FERS signal analyzed to understand the dynamics of bacterial biochemical composition and its correlation with growth stages. Additionally, the platform's performance was validated using diverse microbial strains and real-world liquid samples to assess its suitability for large-scale applications. The results of this study have been published in Biomedical Optics Express.
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Report
(4 results)
Research Products
(13 results)
