| Project/Area Number |
20K12701
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90130:Medical systems-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
Kotsifaki Domna 沖縄科学技術大学院大学, 量子技術のための光・物質相互作用ユニット, 客員研究員 (70834117)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Nanophotonics / Raman spectroscopy / Single-bacterium level / Bacteria detection / Raman Spectroscopy / Nano-apertures / Metallic devices / Bacteria / Plasmonics / Optical Tweesers / Optical Tweezers / Nanoholes / Convection effects / phototoxicity / Optical trapping / Biomedicine / Metamaterials |
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| Outline of Research at the Start |
The interest of single-bacterium analysis has recently gained significant attention.In this proposal, the analysis and monitoring of antibiotic susceptibility at the single-bacterium level, in real-time, will be investigated via a novel platform named Electro-Nanohole Tweezers (ENT). I envision that ENT will be a high potential candidate to supplement or replace existing time-consuming methods such as antimicrobial resistance tests and will help the study to mitigate the challenge of drug resistance in clinical microbiology.
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| Outline of Final Research Achievements |
The distinction between causes of acute infections is a major clinical challenge which is faced by humanity at the dawn of the 21st century. My project identified with high sensitivity the electro-optical properties and phenotypes of a single trapped bacterium by employing a novel approach based on the engagement of material science, nanophotonics and biomedicine in a short timescale. By using nanophotonic tools, I studied bacteria's detection, growth, survival, and reproduction mechanisms suspended in their natural environment to shed light on their behavior. By understanding these processes at the single-micro-organism level, we can gain deeper insights into microbial ecology, antibiotic resistance development, and the fundamental principles of cellular biology.
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| Academic Significance and Societal Importance of the Research Achievements |
Rapid and reliable identification of pathogenic bacteria is crucial across various fields such as healthcare, food safety, and environmental sciences. My research demonstrates a nanophotonic platform capable of providing valuable and repeatable bacterial information in liquid environments.
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