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Carbon and nitrogen dynamics in paddy soils from the perspective of iron-reducing nitrogen-fixing bacteria

Research Project

Project/Area Number 20K15423
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionThe University of Tokyo

Principal Investigator

Masuda Yoko  東京大学, 大学院農学生命科学研究科(農学部), 助教 (80813237)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords水田土壌 / 鉄還元窒素固定菌 / 稲わら / 安定同位体 / SIP / 13C-SIP / 15N-SIP / 鉄還元菌 / GCMS / IRMS / 窒素固定 / LCMS / 炭素循環 / 窒素循環
Outline of Research at the Start

応募者はこれまでに、水田土壌の窒素肥沃度維持の鍵は鉄還元菌窒素固定にあること、水田土壌由来の鉄還元菌が、稲わらの分解により生成される酢酸等の有機酸や、稲わらの構成成分であるセルロースを資化できることを明らかにした。これらのことから、「稲わら由来の炭素化合物が鉄還元菌窒素固定を駆動して土壌の窒素肥沃度を維持させ、その窒素肥沃度に支えられて水稲が生育する」という仮説が導かれた。本研究はこの仮説の検証を目的とし、鉄還元窒素固定菌が稲わら由来の炭素化合物を同化して窒素固定することの証明、ならびにその窒素固定量の解析を行う。本研究は水田における持続的な水稲生産を支えるメカニズムの真相に迫る意義を有する。

Outline of Final Research Achievements

Bacteria utilising rice straw-derived carbon were identified by SIP using paddy soil microcosms supplemented with 13C-labelled rice straw. As a result, iron-reducing bacteria incorporated 13C derived from rice straw from the early stages of culture. Rice straw-derived organic acids and sugars were also identified and quantified using GCMS. The results indicated that rice straw-derived carbon compounds were quickly consumed and little remained in the soil.
Paddy soil microcosms containing 15N2 in the gas phase were prepared to verify that the iron-reducing diazotrophs actually fix nitrogen. The amount of nitrogen fixed in the soil was also calculated using IRMS.
This study revealed that iron-reducing diazotrophs utilise rice straw-derived carbon and increase the nitrogen fertility of paddy soils.

Academic Significance and Societal Importance of the Research Achievements

代表者が発見した「鉄還元菌窒素固定」は、窒素固定の従来の常識を覆す新知見であった。本研究は水田土壌の自律的な窒素肥沃度維持の微生物メカニズムとして、稲が鉄還元菌窒素固定を駆動して土壌の窒素肥沃度(地力)を高め、それを利用して稲が生育するとの新概念、「稲が土を肥やし土が稲を育てる」を提唱し検証したものであり、実際に水田土壌で起きている事象の完全解明という学術的な意義だけでなく、将来的に低窒素施肥農業技術を支える基盤となる社会的意義も持つ。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (24 results)

All 2023 2022 2021 2020

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (15 results) (of which Invited: 5 results)

  • [Journal Article] Active Nitrogen Fixation by Iron-Reducing Bacteria in Rice Paddy Soil and Its Further Enhancement by Iron Application2023

    • Author(s)
      Zhang Zhengcheng、Masuda Yoko、Xu Zhenxing、Shiratori Yutaka、Ohba Hirotomo、Senoo Keishi
    • Journal Title

      Applied Sciences

      Volume: 13 Issue: 14 Pages: 8156-8156

    • DOI

      10.3390/app13148156

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biological nitrogen fixation in the long-term nitrogen-fertilized and unfertilized paddy fields, with special reference to diazotrophic iron-reducing bacteria2023

    • Author(s)
      Masuda Yoko、Satoh Sakura、Miyamoto Ryota、Takano Ryo、Ishii Katsuhiro、Ohba Hirotomo、Shiratori Yutaka、Senoo Keishi
    • Journal Title

      Archives of Microbiology

      Volume: 205 Issue: 8

    • DOI

      10.1007/s00203-023-03631-8

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anaeromyxobacter oryzae sp. nov., Anaeromyxobacter diazotrophicus sp. nov. and Anaeromyxobacter paludicola sp. nov., isolated from paddy soils2022

    • Author(s)
      Itoh Hideomi、Xu Zhenxing、Mise Kazumori、Masuda Yoko、Ushijima Natsumi、Hayakawa Chie、Shiratori Yutaka、Senoo Keishi
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 72 Issue: 10 Pages: 1-10

    • DOI

      10.1099/ijsem.0.005546

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Investigation of Rice Yields and Critical N Losses from Paddy Soil under Different N Fertilization Rates with Iron Application2022

    • Author(s)
      Shen Weishou、Long Yaou、Qiu Zijian、Gao Nan、Masuda Yoko、Itoh Hideomi、Ohba Hirotomo、Shiratori Yutaka、Rajasekar Adharsh、Senoo Keishi
    • Journal Title

      International Journal of Environmental Research and Public Health

      Volume: 19 Issue: 14 Pages: 8707-8707

    • DOI

      10.3390/ijerph19148707

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Geomesophilobacter sediminisgen. nov., sp. nov., Geomonas propionicasp. nov. and Geomonas anaerohicanasp. nov., three novel membersin the family Geobacterecaceisolated from river sediment and paddy soil2021

    • Author(s)
      Zhengcheng Zhang, Zhenxing Xu, Yoko Masuda, Xueding Wang, Natsumi Ushijima, Yutaka Shiratori, Keishi Senoo, Hideomi Itoh
    • Journal Title

      Systematic and Applied Microbiology

      Volume: 44(5) Issue: 5 Pages: 126233-126233

    • DOI

      10.1016/j.syapm.2021.126233

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genome-Based Taxonomic Rearrangement of the Order Geobacterales Including the Description of Geomonas azotofigens sp. nov. and Geomonas diazotrophica sp. nov.2021

    • Author(s)
      Xu Zhenxing、Masuda Yoko、Wang Xueding、Ushijima Natsumi、Shiratori Yutaka、Senoo Keishi、Itoh Hideomi
    • Journal Title

      Frontiers in Microbiology

      Volume: 12

    • DOI

      10.3389/fmicb.2021.737531

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 水田土壌における鉄還元菌窒素固定の学術的基盤解明と低窒素農業への応用2021

    • Author(s)
      妹尾啓史・増田曜子・伊藤英臣・白鳥豊・大峽広智・Xu Zhenxing・山中遥加・石田敬典・高野諒・佐藤咲良・ Shen Weishou
    • Journal Title

      Soil Microorganisms

      Volume: 75 Issue: 2 Pages: 60-65

    • DOI

      10.18946/jssm.75.2_60

    • NAID

      130008109015

    • ISSN
      0912-2184, 2189-6518
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Undervalued pseudo-nifH sequences in public databases distort metagenomic insights into biological nitrogen fixers2021

    • Author(s)
      Kazumori Mise, Yoko Masuda, Keishi Senoo, Hideomi Itoh
    • Journal Title

      mSphere

      Volume: 6 Issue: 6

    • DOI

      10.1128/msphere.00785-21

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enhancement of the nitrogen-fixing activity of paddy soils owing to iron application2021

    • Author(s)
      Masuda Yoko、Shiratori Yutaka、Ohba Hirotomo、Ishida Takanori、Takano Ryo、Satoh Sakura、Shen Weishou、Gao Nan、Itoh Hideomi、Senoo Keishi
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 1 Issue: 3 Pages: 1-5

    • DOI

      10.1080/00380768.2021.1888629

    • NAID

      210000158861

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 鉄で土を肥やす! 鉄還元に伴う窒素固定の発見2022

    • Author(s)
      増田曜子
    • Organizer
      新潟県土壌肥料懇話会 第2回研究会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 鉄で土を肥やし環境を守る:水田の持続的水稲生産を支える鉄還元菌窒素固定の発見と応用2022

    • Author(s)
      増田曜子
    • Organizer
      農学と工学の超越創発 イノベーションフォーラム
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 水田土壌において鉄還元窒素固定菌への炭素源供給を担う微生物群集の解明2022

    • Author(s)
      笠原拓己、増田曜子、妹尾啓史
    • Organizer
      日本土壌肥料学会
    • Related Report
      2022 Research-status Report
  • [Presentation] 稲が土を肥やし土が稲を育てる -鉄還元菌と水田土壌の炭素・窒素循環-2022

    • Author(s)
      増田曜子
    • Organizer
      日本微生物生態学会
    • Related Report
      2022 Research-status Report
  • [Presentation] 鉄還元窒素固定菌による水稲由来炭素化合物の利用2022

    • Author(s)
      千原 光貴, 増田 曜子, Xu Zhenxing, 妹尾 啓史
    • Organizer
      日本微生物生態学会
    • Related Report
      2022 Research-status Report
  • [Presentation] 水田土壌において鉄還元窒素固定菌への炭素源供給を担う微生物群の解明2022

    • Author(s)
      笠原拓己、増田曜子、妹尾啓史
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 鉄で土を肥やし環境を守る:水田の持続的水稲生産を支える鉄還元菌窒素固定の発見と応用2022

    • Author(s)
      増田曜子
    • Organizer
      農学と工学の超越創発イノベーションフォーラム
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 鉄で土を肥やす!鉄還元に伴う窒素固定の発見2022

    • Author(s)
      増田曜子
    • Organizer
      新潟県土壌肥料懇話会 令和3年度第2回研究会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 水田土壌において鉄還元窒素固定菌への炭素源供給を担う微生物群集の解明2021

    • Author(s)
      笠原拓己、増田曜子、妹尾啓史
    • Organizer
      日本微生物生態学会
    • Related Report
      2021 Research-status Report
  • [Presentation] DNRA-derived N2O production and its isotopomer signatures in Geomonas and Oryzomonas, iron reducing bacteria isolated from paddy soils2021

    • Author(s)
      許 振興、増田曜子、伊藤英臣、服部祥平、豊田 栄、妹尾啓史
    • Organizer
      日本微生物生態学会第34回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 木(=bacteria)を見て森(=microbiome)も見るために。2021

    • Author(s)
      増田曜子
    • Organizer
      Plant Microbiota Research Network (PMPR) 第1回シンポジウム
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 窒素長期無施用水田土壌における窒素肥沃度維持の微生物メカニズム2021

    • Author(s)
      増田曜子
    • Organizer
      2021年度 日本土壌肥料学会 関東支部大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Identification of activated diazotrophs by iron application in paddy soil using 15N2 -DNA Stable-Isotope Probing coupled with amplicon sequencing2021

    • Author(s)
      Zhang Zhengcheng, Yoko Masuda, Keishi Senoo
    • Organizer
      日本微生物生態学会第34回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 水田土壌における鉄還元菌窒素固定の発見・検証と鉄・稲わら添加による増強2020

    • Author(s)
      増田曜子、石田敬典、山中遥加、Xu Zhenxing、伊藤英臣、白鳥豊、大峡広智、妹尾啓史
    • Organizer
      土壌肥料学会
    • Related Report
      2020 Research-status Report
  • [Presentation] 水田土壌の鉄還元菌窒素固定の増強-稲わらと鉄添加の効果2020

    • Author(s)
      増田曜子、石田敬典、伊藤英臣、早川智恵、白鳥豊、妹尾啓史
    • Organizer
      土壌微生物学会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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