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Analysis of reducing mechanisms of solid-phase humic substances by unknown extracellular electron transfer pathways of bacteria.

Research Project

Project/Area Number 20K15431
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionNagoya University

Principal Investigator

Kasai Takuya  名古屋大学, 未来材料・システム研究所, 助教 (00833831)

Project Period (FY) 2020-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords固体腐植物質 / 微生物電気化学 / 細胞外電子伝達 / 固体腐植ヒューミン / 細胞外電子伝達系 / 種間電子伝達
Outline of Research at the Start

ヒューミンとはいかなるpHにも不溶な固体腐植物質である。これまでにヒューミンは電子伝達物質として機能し、ヒューミン酸化菌の異化代謝系を活性化させること、ヒューミン自体の物質的電気化学的特性について報告されている。
本研究では、電気化学的・分子生物学的手法を用いて、Shewanella oneidensis MR-1株における生物学的ヒューミン還元機構の解明を目的とする。本研究で得られる成果は、ヒューミンを介した種間電子伝達機構の全容を解明する上で非常に重要であるだけでなく、環境中に普遍的に存在するヒューミンを利用する微生物の生態や生存戦略の理解を助ける知見となると考えられる。

Outline of Final Research Achievements

In this study, we investigated mechanisms of reduction of solid phase humic substances by microorganisms and revealed microorganisms related in humin reduction. Specifically, we have carried out (i) examination of mechanism of humin reduction using Shewanella oneidensis MR-1 as a model electrochemically active bacterium and (ii) identification of humin reducing bacteria using a next generation sequencer.

Academic Significance and Societal Importance of the Research Achievements

ヒューミンは、土壌や底泥に普遍的に存在する有機無機複合物であり、電子伝達物質として様々な微生物反応を促進することが報告されている。環境中では、ヒューミンを介した微生物間共生が予想されるが、ヒューミン還元菌に関する知見は全くない。また、ヒューミンの利用には既知の細胞外電子伝達系を持たない多様な微生物が関与することが示唆されているため、微生物電気化学システム(BES)への応用が期待されるが、その電子伝達機構も不明である。これらの課題を明らかにするためヒューミン還元機構に着目して研究を実施した。本成果は、環境微生物生態の一角の解明および、多様な微生物を利用できるBESの構築が期待される

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • Research Products

    (16 results)

All 2022 2021

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (13 results) (of which Int'l Joint Research: 9 results,  Invited: 2 results)

  • [Journal Article] Effect of Humin and Chemical Factors on CO2-Fixing Acetogenesis and Methanogenesis2022

    • Author(s)
      Ha Biec Nhu、Pham Duyen Minh、Kasai Takuya、Awata Takanori、Katayama Arata
    • Journal Title

      International Journal of Environmental Research and Public Health

      Volume: 19 Issue: 5 Pages: 2546-2546

    • DOI

      10.3390/ijerph19052546

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Promotion of biological nitrogen fixation activity of an anaerobic consortium using humin as an extracellular electron mediator2021

    • Author(s)
      Dey Sujan、Awata Takanori、Mitsushita Jumpei、Zhang Dongdong、Kasai Takuya、Matsuura Norihisa、Katayama Arata
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 6567-6567

    • DOI

      10.1038/s41598-021-85955-3

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Humin: No longer inactive natural organic matter2021

    • Author(s)
      Pham Duyen Minh、Kasai Takuya、Yamaura Mirai、Katayama Arata
    • Journal Title

      Chemosphere

      Volume: 269 Pages: 128697-128697

    • DOI

      10.1016/j.chemosphere.2020.128697

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 固体フミン質を適用した微生物電気化学システムの構築と最適化2022

    • Author(s)
      増田大起,Pham Duyen Minh,笠井拓哉,片山新太
    • Organizer
      第56回日本水環境学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 固体腐植ヒューミンを用いた生物電気化学システムにおける酢酸生成菌よる二酸化炭素変換の促進2022

    • Author(s)
      笠井 拓哉,伊藤 啓人,Pham Minh Duyen,菰田 峰生,片山 新太
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Alternate functionality of humin, a solid-phase humic substance, as extracellular electron mediator in carbon dioxide-reducing acetogenesis2021

    • Author(s)
      Arata Katayama, Mahasweta Laskar, Biec Nhu Ha, Duyen Minh Pham, Takuya Kasai
    • Organizer
      American Chemical Society, Spring 2021 meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activation of carbon dioxide reduction by autotrophic acetogen by solid-phase humic substances2021

    • Author(s)
      Takuya Kasai, Mahasweta Laskar, Arata Katayama
    • Organizer
      World Microbe Forum 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Acceleration of Biological N-fixation by a Solid-phase electron mediator, humin2021

    • Author(s)
      Sujan Dey, Takuya Kasai, Arata Katayama
    • Organizer
      World Microbe Forum 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activity of CO2-Fixing Acetogenesis and Methanogenesis in Mixed Cultures with Humin under Different Conditions2021

    • Author(s)
      Biec Nhu HA, Duyen Minh PHAM, Takuya KASAI, Arata KATAYAMA
    • Organizer
      The Water and Environment Technology Conference Online2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Extracellular Electron Mediating Functions Discovered in Fresh Organic Materials and Changes the Functionality in Artificial Soil2021

    • Author(s)
      Tingting HU, Mirai YAMAURA, Duyen minh PHAM, Takuya KASAI, Arata KATAYAMA
    • Organizer
      The Water and Environment Technology Conference Online2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Humin Promotes the Biological-H2 (Bio-H2) Production of an Anaerobic Consortium Enriched Under Nitrogen-deficient Condition2021

    • Author(s)
      Sujan Dey, Takuya Kasai, Arata Katayama
    • Organizer
      International Conference on Materials and Systems for Sustainability 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Study on extracellular electron transfer mechanism in humin promoting redox reactions of nitrogen2021

    • Author(s)
      Takuya Kasai, Sujan Dey, Takehito Noto, Ryouta Ujibayashi and Arata Katayama
    • Organizer
      International Conference on Materials and Systems for Sustainability 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effects of chemical factors and microbial community on the activity of CO2-fixing acetogenesis and methanogenesis with the presence of humin as extracellular electron mediator2021

    • Author(s)
      Biec Nhu Ha, Duyen Minh Pham, Takuya Kasai, Takanori Awata, Arata Katayama
    • Organizer
      International Conference on Materials and Systems for Sustainability 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Strategy of anaerobic bioremediation: design of artificial microbial community and bioelectrochemical system2021

    • Author(s)
      Arata Katayama, Takuya Kasai, Naoko Yoshida
    • Organizer
      the 41st International Symposium on Halogenated Persistent Organic Pollutants (Dioxin 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Increase in Extracellular Electron Mediating Function of Rice Straw-artificial Soil Mixture During the Humification2021

    • Author(s)
      Tingting Hu, Mirai Yamaura, Duyen Minh Pham, Takuya Kasai, Arata Katayama
    • Organizer
      日本微生物生態学会第34回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 微生物によるヒューミン還元機構の解析とそれに関わる微生物の同定2021

    • Author(s)
      能登 健仁, 笠井 拓哉, 片山 新太
    • Organizer
      日本微生物生態学会第34回大会
    • Related Report
      2021 Annual Research Report

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Published: 2020-04-28   Modified: 2023-01-30  

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