| Project/Area Number |
20K15466
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Ben Othman Mahmoud 筑波大学, 健幸イノベーション開発研究センター, 研究員 (40868337)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | anti-fatigue / muscles atrophy / treadmill / power strentgh / C2C12 cells / Muscle atrophy / Treadmill / Power strength / Fatigue / FST / Lactic acid / ATP / Glycogen / Bifidobacterium longum / mice |
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| Outline of Research at the Start |
This research will uncover the effects of the inactivated Bifidobacterium longum (IBL) on muscles atrophy and physical fatigue using both models in vitro and in vivo. The glucocorticoid, dexamethasone (Dex) will be used as inducer of myotube atrophy in C2C12 cells. In addition, C57BL/6J mice will exposed to different types of exercises and tests (forced swimming test (FST), running (treadmill), fasting (hunger) and hindlimb (inactivation)) to prove the potential beneficial effects of IBL on the alleviation of muscles atrophy and physical fatigue.
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| Outline of Final Research Achievements |
In order to evaluate the anti-fatigue effect of inactivated Bifidobacterium longum (IBL) extract, some experiments were performed on mice. First of all, mice were exposed to running test using treadmill. Then, we investigate the effect of IBL on immobilization by applying the hindlimb test. The results showed a significant increase of the running distance of treated mice compared with non-treated mice. In addition, the results demonstrated a remarkable increase of power strength in treated groups. Moreover, muscles weight was improved in IBL-treated groups.
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| Academic Significance and Societal Importance of the Research Achievements |
our research showed that IBL can be used for anti-fatigue and muscles atrophy in mice. however, it can be used for human treatment. People who suffering from serious injuries and immobility period due to hospitalization, BL can be a relative treatment.
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