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Verification of epigenetic abnormalities in hybrid endosperm and estimation of its mechanism

Research Project

Project/Area Number 20K15504
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 39010:Science in plant genetics and breeding-related
Research InstitutionYokohama City University (2022)
Iwate University (2020-2021)

Principal Investigator

Tonosaki Kaoru  横浜市立大学, 木原生物学研究所, 助教 (20749494)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords生殖的隔離 / 野生イネ / 胚乳 / ゲノムインプリンティング / エピゲノム / イネ / 胚乳発生
Outline of Research at the Start

種間交雑で見られる胚乳発生の異常は、雑種形成を妨げる生殖的隔離の主要因であり、その打破手法の確立が求められる。しかし、様々な植物種における交雑実験の結果から、種間交雑によって生じる胚乳発生の異常には、一定の法則性が見出されているものの、その分子メカニズム解明には至っていない。申請者のイネを用いたこれまでの研究から、雑種胚乳ではエピジェネティックな制御機構(エピゲノム)の異常が生じており、胚乳発生の異常の原因である可能性を見出してきた。そこで本研究では、種間交雑によって引き起こされるエピゲノムの異常の検証とその原因解明により、胚乳における生殖的隔離の分子メカニズムに迫る。

Outline of Final Research Achievements

The interspecific crossing between cultivated rice and wild rice shows lethal phenotypes in hybrid seeds due to the hybrid endosperm abnormality. Our study revealed that almost paternally imprinted genes were de-repressed in the hybrid endosperm. We also revealed a repressive histone mark, H3K27me3, controls the mono-allelic expression pattern of paternally imprinted genes in rice endosperm. In addition, we succussed in overcoming the hybridization barrier using mutant rice of OsEMF2a involved in catalytic H3K27me3. These results show that H3K27me3 involves the hybridization barrier in rice endosperm.

Academic Significance and Societal Importance of the Research Achievements

胚乳で見られる生殖的隔離は、様々な植物種で観察されるものの、その分子メカニズム解明には至っていなかった。本研究では、その分子メカニズム解明への足がかりとなる成果を得た。また、物種に近縁な野生種は、既存の栽培種には存在しない様々な環境適応能力を保持した優良な遺伝資源であるが、生殖的隔離のために遺伝資源のほとんどが利用されていないのが現状であった。本研究成果によって野生イネとの種間交雑を打破することに成功したことから、これまでは育種利用が不可能であった野生イネを遺伝資源として利用可能となることが期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (10 results)

All 2023 2022 2021 2020

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Multi-layered epigenetic control of persistent and stage-specific imprinted genes in rice endosperm2023

    • Author(s)
      Tonosaki Kaoru、Ono Akemi、Nagata Hiroki、Furuumi Hiroyasu、Nonomura Ken-ichi、Sato Yutaka、Comai Luca、Hatakeyama Katsunori、Kawakatsu Taiji、Kinoshita Tetsu
    • Journal Title

      Research Square

      Volume: -

    • DOI

      10.21203/rs.3.rs-2403620/v1

    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Journal Article] Comparative Evaluation of Three Commercial Quantitative Real-Time PCRs Used in Japan for Bovine Leukemia Virus2022

    • Author(s)
      Yoneyama Syuji、Kobayashi Sota、Matsunaga Towa、Tonosaki Kaoru、Leng Dongze、Sakai Yusuke、Yamada Shinji、Kimura Atsushi、Ichijo Toshihiro、Hikono Hirokazu、Murakami Kenji
    • Journal Title

      Viruses

      Volume: 14 Issue: 6 Pages: 1182-1182

    • DOI

      10.3390/v14061182

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Will epigenetics be a key player in crop breeding?2022

    • Author(s)
      Tonosaki Kaoru、Fujimoto Ryo、Dennis Elizabeth S.、Raboy Victor、Osabe Kenji
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 958350-958350

    • DOI

      10.3389/fpls.2022.958350

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Temporal changes in transcripts of miniature inverted-repeat transposable elements during rice endosperm development2022

    • Author(s)
      Hiroki Nagata, Akemi Ono, Kaoru Tonosaki, Taiji Kawakatsu, Yutaka Sato, Kentaro Yano, Yuji Kishima, Tetsu Kinoshita
    • Journal Title

      The Plant Journal

      Volume: 109 Issue: 5 Pages: 1035-1047

    • DOI

      10.1111/tpj.15698

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mutation of the imprinted gene OsEMF2a induces autonomous endosperm development and delayed cellularization in rice2021

    • Author(s)
      Tonosaki Kaoru、Ono Akemi、Kunisada Megumi、Nishino Megumi、Nagata Hiroki、Sakamoto Shingo、Kijima Saku T、Furuumi Hiroyasu、Nonomura Ken-Ichi、Sato Yutaka、Ohme-Takagi Masaru、Endo Masaki、Comai Luca、Hatakeyama Katsunori、Kawakatsu Taiji、Kinoshita Tetsu
    • Journal Title

      The Plant Cell

      Volume: 33 Pages: 85-103

    • DOI

      10.1093/plcell/koaa006

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Multi-layers of epigenetic controls in the genomic imprinting during rice endosperm development2022

    • Author(s)
      Kaoru Tonosaki, Akemi Ono, Hiroyasu Furuumi, Yutaka Sato, Taiji Kawakatsu, Tetsu Kinoshita
    • Organizer
      CSHA Integrative Epigenetics in Plants
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネの胚乳発生過程におけるゲノムインプリンティングの多層的なエピゲノム制御2022

    • Author(s)
      殿崎薫, 木下哲
    • Organizer
      第45回日本分子生物学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] The factor of transcriptional repression regulating endosperm development in rice2021

    • Author(s)
      殿崎薫
    • Organizer
      第62回 日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] イネ胚乳発生を制御するポリコーム複合体の標的遺伝子の同定2021

    • Author(s)
      殿崎薫、小野明美、古海弘康、野々村賢一、佐藤豊、遠藤真咲、Luca Comai、畠山勝徳、川勝泰二、木下 哲
    • Organizer
      日本育種学会第139回講演会
    • Related Report
      2020 Research-status Report
  • [Presentation] 栽培イネと野生イネにおけるインプリントームの比較2020

    • Author(s)
      殿崎薫、小野明美、永田博基、古海弘康、野々村賢一、川勝泰二、佐藤 豊、Luca Comai、木下 哲
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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